Sequence Import Configure Reference

Sequence Import Configure is a tiny application that saves a file on the instrument computer, which allows to automatically import .raw files into a Skyline file after the .raw file acquisition is finished, freeing you from manual File / Import / Result operations.

Steps to configure .raw file import to a Skyline file

1. Run this program. There's no options, just run it. The program finds the SkylineCmd.exe application path, and saves it to a fixed location so that the instrument software can use it to import .raw files.
   

2. In a future Xcalibur 4.8 release, there will be a native Skyline column, that has a file chooser, and does some simple validation. This column will be accessed by a future Stellar MS software, 1.2.

   • For Xcalibur < 4.8 and Stellar 1.1 software the following few steps should be taken:
   1. Add Skyline to an Xcalibur "User Label".
   2. Configure the new Skyline column by adding it to the Active columns.
   3. In the Xcalibur sequence, add the full file path to an existing Skyline file, including the .sky extension. For example, D:\MyFiles\skyline_test.sky

That's it!

• The import task has to wait for the LC to finish doing what it's doing, for the .raw file to close and become available for import. This can take a while, even 5-10 minutes, depending on your LC setup and equilibration settings.
• Note that if you have the Skyline file open when the acquisition ends, we will raise a little dialog telling you that we can't import the .raw files until you close the Skyline file.
• We'll keep a queue of the pending import jobs for up to 72 hours, then we'll give up.
• If you manually imported the .raw file, we will not import it again.