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Table SI 1

EAWAG Environmental Toxicology - mTOR_zf_PAC2_growth_curve

Table SI 1

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Table SI 1: List of Tartget Proteins, Phosphosites and Corresponding (Phospho)peptide Targets      
Modifications are indicated as alkylated cysteine (+57) and phosphorylation (+80). Additional information provided for each peptide includes: Amino acid (aa) length of the peptide; Assay basis: indicating whether the assay is based on light peptides, heavy isotope-labeled peptides, or spectral library; Intended use of the peptide: Protein abundance measurement, phosphorylation (P) site quantification, non-phosphorylated peptide with putative phosphosite (counterpart) or quality control (QC) purposes. 
zebrafish
gene		 description protein accession ID putative modification peptide modified sequence [sequence range] aa length assay based on used for
actba Actin, cytoplasmic 1 Q7ZVI7   K.AGFAGDDAPR.A [19, 28] 9 heavy abundance
        R.GYSFTTTAER.E [197, 206] 10 heavy normalization of total protein amount
akt1 non-specific serine/threonine protein kinase 1 M4MD44   K.EGITDGATMK.T [292, 301] 10 light abundance
        K.FFTGIVWQDVYEK.K [401, 413] 13 heavy abundance
      S467 R.RPHFPQFS[+80]YSASGTA.- [460, 474] 15 heavy P
        R.RPHFPQFSYSASGTA.- [460, 474] 15 light counterpart
akt2 non-specific serine/threonine protein kinase 2 A0JMK1   K.EGITNEATMK.T [297, 306] 10 heavy abundance
      S472 R.THFSQFS[+80]YSASVR.E [466, 478] 13 heavy P
        R.THFSQFSYSASVR.E [466, 478] 13 light counterpart
akt3 non-specific serine/threonine protein kinase 3 D9IL79   K.DEVAHTLTESR.V [188, 198] 11 heavy QC
        K.EGITDAATMK.T [295, 304] 10 light abundance
akt1/2/3 non-specific serine/threonine protein kinase 1/2/3   T302 K.T[+80]FC[+57]GTPEYLAPEVLEDNDYGR.A [302, 322] 20 light P
        K.TFC[+57]GTPEYLAPEVLEDNDYGR.A [302, 322] 20 heavy counterpart
akt1s1  AKT1 substrate 1 (proline-rich) / PRAS40 E7EY34   K.FHPSGIEVEGQR.N [52, 63] 11 light abundance
      S284 R.S[+80]LPVSVPVWGYR.N [284, 295] 12 heavy P
        R.SLPVSVPVWGYR.N [284, 295] 12 light counterpart
        R.VGC[+57]ADLDHIAASMK.A [310, 323] 13 light counterpart
      T347 R.LNT[+80]GDFSLK.H [345, 353] 9 heavy P
        R.LNTGDFSLK.H [345, 353] 9 light counterpart
eif4ebp1 eukaryotic Translation initiation factor 4E-binding protein 1 Q6PFS8 S62 K.FLLDC[+57]RSS[+80]PLAR.T [55, 66] (missed 1) 12 heavy QC
      S62 R.SS[+80]PLAR.T [61, 66] 6 heavy P
        K.AYPKPTVNNNSISPPVDK.S [91, 108] 19 light counterpart
        K.STGEDAQFEMDI.- [109, 120] 12 light abundance
erk2 Extracellular signal-regulated kinase 2   R.VADPDHDHTGFLTEYVATR.W [182, 200] 19 light counterpart
        R.NYLLSLPLR.S [271, 279] 9 light abundance
fkbp8 peptidylprolyl isomerase Q6P3J6 Y225 R.GNVHY[+80]QR.A [221, 227] 7 heavy P
        K.VLALQGEFAEAIK.T [308, 320] 13 light abundance
      Y357 K.GAEQAMY[+80]K.K [351, 358] 8 heavy P
gapdh Glyceraldehyde-3-phosphate dehydrogenase Q5XJ10   K.VGINGFGR.I [4, 11] 8 heavy QC
hif1ab Hypoxia-inducible factor 1-alpha Q6EHI4 S458 K.LPLSLS[+80]PLTPSDSIPALTK.L [453, 471] 19 heavy P
        K.LPLSLSPLTPSDSIPALTK.L [453, 471] 19 light counterpart
        K.TILILPSDVASR.L [720, 731] 12 light abundance
lamtor1 Ragulator complex protein LAMTOR1 E7FBZ6 Y139 K.IAAYAY[+80]SAISQIK.V [134, 146] 13 heavy QC
lamtor2 Ragulator complex protein LAMTOR2 F1R3H0 S125 K.AEALVQYLEEPLTQVAAS[+80].- [108, 125] 18 heavy P
lamtor5 Ragulator complex protein LAMTOR5 E7FA90 S38 R.GSLS[+80]DEHGGVVSVLAK.Q [35, 50] 16 heavy P
        R.GSLSDEHGGVVSVLAK.Q [35, 50] 16 light counterpart
        R.HGTITLAVHK.M [79, 88] 16 light abundance
lpin1a phosphatidate phosphatase Q14EB8   R.SDTLGHILPTLGK.D [693, 705] 13 light abundance
        K.NLFYPNAEPFYAAFGNR.A [792, 808] 17 light counterpart
      Y802 K.NLFYPNAEPFY[+80]AAFGNR.A [792, 808] 17 heavy P
lpin1 isoform X1  phosphatidate phosphatase A0A8M9P333 Y382 R.HLGSDGVY[+80]LDDIK.D [375, 387] 13 heavy P
      Y397 K.DLEPEVAALY[+80]FPK.S [388, 400] 13 heavy P
        K.DLEPEVAALYFPK.S [388, 400] 13 light counterpart
      Y761 R.NLFLPNTEPFY[+80]AAFGNR.D [751, 767] 17 heavy P
        R.LGEVVDHVFPLLK.R [808, 820] 13 light abundance
mlst8 Target of rapamycin complex subunit lst8 Q803V5 S43 R.TVQHQDS[+80]QVNSLEVTPDR.S [37, 54] 18 heavy P
        R.TVQHQDSQVNSLEVTPDR.S [37, 54] 18 light counterpart
        R.SMIAAAGYQHIR.M [55, 66] 12 light abundance
mTOR Serine/threonine-protein kinase TOR Q06RG6   R.VLGLLGALDPYK.H [870, 881] 12 heavy abundance
      S1244 K.LHVS[+80]TTALQK.A [1241, 1250] 10 heavy P
        K.LHVSTTALQK.A [1241, 1250] 10 light counterpart
      S1401 K.GASPLILESLIS[+80]INNK.L [1390, 1405] 16 heavy P
        K.GASPLILESLISINNK.L [1390, 1405] 16 light counterpart
pak1 Serine/threonine-protein kinase PAK 1   R.SVIEPLPPPLSTK.D [203, 215] 13 light counterpart
        K.LTDFGFC[+57]AQITPEQSK.R [437, 452] 16 light abundance
PPAR[g] Peroxisome proliferator-activated receptor gamma A6XMH6 S128 K.LEPES[+80]PPQFAENSVSFSK.T [124, 141] 18 heavy P
prkaa1 Acetyl-CoA carboxylase kinase / AMPK1 A5WUM0   R.HTLDELNPQK.S [381, 390] 10 light abundance
        K.MSLQLYQVDSR.T [452, 462] 11 light counterpart
      S462 K.MSLQLYQVDS[+80]R.T [452, 462] 11 heavy P
        K.SGTATPHR.S [480, 487] 8 light counterpart
      T484 K.SGTAT[+80]PHR.S [480, 487] 8 heavy P
        R.SGSVGNYR.T [488, 495] 8 light counterpart
      S490 R.SGS[+80]VGNYR.T [488, 495] 8 heavy P
prkaa2 5'-AMP-activated protein kinase catalytic subunit alpha-2 / AMPK2 E7F9C4 S490 R.SGS[+80]TAGLLRPR.L [488, 498] 11 heavy P
prkaa1/2       K.IADFGLSNMMSDGEFLR.T [156, 172] 17 light counterpart
      S162 K.IADFGLS[+80]NMMSDGEFLR.T [156, 172] 17 heavy P
        R.TSC[+57]GSPNYAAPEVISGR.L [173, 189] 17 light counterpart
      T173 R.T[+80]SC[+57]GSPNYAAPEVISGR.L [173, 189] 17 heavy P
prkag1 5'-AMP-activated protein kinase subunit gamma-1 / AMPKg Q6PCS7   K.LVVFDTSLQVK.K [46, 56] 11 light abundance
        R.SQYFEGVLTC[+57]R.A [268, 278] 11 light counterpart
      Y270 R.SQY[+80]FEGVLTC[+57]R.A [268, 278] 11 heavy P
        R.ASETLQAIINR.L [279, 289] 11 light counterpart
      T282 R.ASET[+80]LQAIINR.L [279, 289] 11 heavy P
rheb GTP-binding protein Rheb Q6PC18   K.LLDMVGK.V [103, 109] 7 light abundance
        R.VISC[+57]EEGK.A [128, 135] 8 light counterpart
      S130 R.VIS[+80]C[+57]EEGK.A [128, 135] 8 heavy P
rictorb rapamycin-insensitive companion of mTOR isoform X1 A0A8M3B4Q4 S1195 K.TLDTQDSTSS[+80]IGEQEVR.L [1186, 1202] 17 heavy P
      S1436 K.ALS[+80]FASLDK.E [1434, 1442] 9 heavy P
      S1631 R.S[+80]TELVLGVK.N [1631, 1639] 9 heavy P
rps18 Ribosomal protein S18 AAH62289   R.AGELTEDEVER.V [56, 66]   library normalization of total protein amount
rps6 40S ribosomal protein S6     K.LIEVDDER.K [15, 22] 8 heavy abundance
      S240 R.RLSSLRAS[+80]TSK.S [233, 243] (missed 2) 11 heavy P
      S236 R.LSS[+80]LRASTSK.S [234, 243] (missed 1) 10 heavy P
      S235, S240 R.LS[+80]SLRAS[+80]TSK.S [234, 243] (missed 1) 10 light P
rps6kb1b Ribosomal protein S6 kinase Q802Y7   K.FTSQTPVDSPDDTK.L [360, 373] 14 light counterpart
      T364 K.FTSQT[+80]PVDSPDDTK.L [360, 373] 14 heavy P
      S415 R.KVLGS[+80]PR.T [411, 417] (missed 1) 7 heavy P
      S415 K.VLGS[+80]PR.T [412, 417] 6 heavy P
        R.TPVSPLK.A [418, 424] 7 light counterpart
      S421 R.TPVS[+80]PLK.A [418, 424] 7 heavy P
        K.AEVEDGWSR.G [425, 433] 9 light abundance
rptor Regulatory-associated protein of MTOR, complex 1 A0A0R4IFI0 K.ALETIGANLQK.Q [100, 110] 11 heavy abundance
        R.SVNSYTNLR.A [722, 730] 9 light counterpart
      S725 R.SVNS[+80]YTNLR.A [722, 730] 9 heavy P
        R.ILDSGSLTQSAPASPTSK.G [852, 869] 18 light counterpart
      S865 R.ILDSGSLTQSAPAS[+80]PTSK.G [852, 869] 18 heavy P
        R.SLVAAGLGDGSVR.V [1207, 1219] 13 light abundance
stk11 non-specific serine/threonine protein kinase Q568P5   R.IDSTEVIYQPR.R [28, 38] 11 light counterpart
      S30 R.IDS[+80]TEVIYQPR.R [28, 38] 11 heavy P
        R.GMLEYDPVK.R [287, 295] 9 light abundance
tsc1b TSC complex subunit 1b E7FEJ0   R.EQLNVGDLLPLLETSDLR.Q [4, 21] 18 light abundance
      S437 R.STAS[+80]PPC[+57]K.D [434, 441] 8 heavy P
      S1139 K.SES[+80]QC[+57]DEEETPPPR.L [1137, 1150] 14 heavy P
tsc2 TSC complex subunit 2 A0A0G2KHX4 K.NTPTLVLPSFYK.A [147, 158] 12 light abundance
        R.STSLNERPK.S [769, 777] 9 light counterpart
      S771 R.STS[+80]LNERPK.S [769, 777] 9 heavy P
        R.SISVSEHAVR.R [811, 820] 10 light counterpart
      S813 R.SIS[+80]VSEHAVR.R [811, 820] 10 heavy P
        R.SISGGHALR.A [962, 970] 9 light counterpart
      S964 R.SIS[+80]GGHALR.A [962, 970] 9 heavy P
        K.SSSSPELQTLPEAFSK.A [1240, 1255] 16 light counterpart
      S1240 K.S[+80]SSSPELQTLPEAFSK.A [1240, 1255] 16 heavy P
        R.GHTISVSAPSSR.R [1327, 1338] 12 light counterpart
      T1329 R.GHT[+80]ISVSAPSSR.R [1327, 1338] 12 heavy P
        R.YAQFLTGLGK.L [1436, 1445] 10 light abundance
ulk1b non-specific serine/threonine protein kinase E9QIB7 S551 R.LNS[+80]APC[+57]LLEAAGGC[+57]R.Q [549, 563] 15 heavy P
        R.NPLPTILGSPSR.A [600, 611] 12 light abundance
      S721 R.VVFTIGS[+80]PPSGATPPQNSR.S [715, 733] 19 heavy P

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 Table_SI_1.txt

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