Figure SI 3: Non-phosphorylated peptide recovery from non-bound fraction obtained using NTA-Fe³⁺ enrichment in different matrices.

Individual recoveries for55 light and 7 heavy synthetic peptides spiked in the solvent matrix (blank, n=3 independent replicates) and 7 heavy peptides (bold) spiked in the zebrafish PAC2 cell lysate matrix (n=6 independent replicates). Boxplots show the interquartile range, representing the middle (50%) of the data, which fall between the upper quartile (75% data below that score) and the lower quartile (less than 25% below that score). The whiskers extend to the minimum and maximum values within each dataset.