Figure SI 2: Phosphopeptide recovery from the bound fraction obtained with TiO2 or NTA-Fe3+ based enrichment methods in different matrices. Individual recoveries are shown for 46 spiked synthetic phosphopeptides processed in the solvent matrix with both enrichment methods (n=3 independent replicates) and for 45 spiked synthetic phosphopeptides in the zebrafish PAC2 cell lysate matrix processed with NTA-Fe³⁺ enrichment method (n=6 independent replicates). Boxplots show the interquartile range, representing the middle (50%) of the data, which fall between the upper quartile (75% data below that score) and the lower quartile (less than 25% below that score). The whiskers extend to the minimum and maximum values within each dataset.