Our Services:

Plasma/Serum Extracellular Vesicle ProteomicsTo overcome the dynamic range challenges of plasma/serum we have developed a robust platform for the enrichment of extracellular vesicles, protein cleanup, and digestion of proteins for mass spectrometry. Our Mag-Net protocol enables deep and robust quantification of proteins. Our methodology is a promising option to support research projects from clinical samples or experiments in non-human specimens (e.g. mice, dogs, non-human primates, etc... For more details on the Mag-Net method and for frequently asked questions, please refer to the following sections:

• Mag-Net method summary
• Frequently asked questions.

 

 

Unfractionated Plasma, Serum, or Cerebrospinal Fluid (CSF) While dynamic range and coverage of biofluids is important, we recognize that it is a subfraction and some will prefer a quantitative measurement that reflects the abundance in the total and unfractionated sample.

---

Targeted Assay Development

We offer the expertise to develop and validate customized multiplex targeted protein assays. We have developed a workflow based on gas phase fractionation-data independent acquisition to expedite the selection of target peptides that provide robust quantitative measurements directly within the sample matrix. We can go from candidate list to scheduled peptide assay in a short time pending the peptides are above the limit of detection. All assays are developed using:

• Single point external calibration
• Assessment of both intra and interbatch precision.
• Quantitative linearity and lower limit of quantitation using matrix matched calibration curves.
• We can make highly multiplex assays using the novel Stellar MS.

 

If you have a question that isn't addressed in the above sections, check out our FAQs. For additional questions, please feel free to email us at services@maccosslab.org.