Prospective cohort studies and meta-analyses examining the relationship between high-density lipoprotein-cholesterol (HDL-C) and stroke risk are discordant and question the value of HDL-C as a marker for stroke risk prediction. We investigated changes in HDL protein composition and function -cholesterol efflux capacity (CEC)- after acute ischemic stroke, and their relationship to long-term recovery after stroke. Protein abundance was assessed by PRM analysis of at least 2 peptides per protein, with normalization to total ion current and spiked-in N15 APOA1 peptides. We find post-stroke event HDL proteome remodeling is dynamic, with distinct time-dependent changes in proteins that predict recovery from stroke.
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