OHSU Pamir Lab - High-density lipoprotein proteome post-stroke

OHSU Pamir Lab - High-density lipoprotein proteome post-stroke

HDL peptides were pooled for preliminary analysis by DDA. 3 pooled samples were created for both the control patient samples and 24 h post stroke samples, while 2 pooled samples were created for the 96 h post stroke samples. Pooled samples were created from combining equal volumes from a subset of 10 samples per experimental group. ProteomeDiscoverer reports are included for each run, as is a combined, filtered results table for 97 shared proteins. Additional ProteomeDiscoverer results files can be found at the bottom of the page.

  Attached Files  
   
 SupplementalTable_2.xlsx

HDL peptide PRM results were imported into Skyline, where peaks were manually inspected and adjusted to ensure comparable integration for peptides used for quantitation across all samples. Fragment areas were exported and normalized for TIC and to [N15] APOA1 peptides. A sample key is provided with basic sample and run information, the Skyline export results and normalization results are included, and the inclusion list for the assay is included.

  Attached Files  
   
 SupplementalTable_3.xlsx