Supplementary Materials

• Scripts and metadata used to create the figures in the paper are in the DataProcessing folder that can be found by clicking the Raw Data button on the top right of the page.

• A tutorial on how to use PRM Conductor can be found here.

• The PRM Conductor external tool can be installed in various ways:

  1. From the Skyline website
  2. From within Skyline using Tools / Tools Store
  3. The instrument control software, in its full and Workstation versions, contains the file _C:\Program Files\Thermo Scientific\Instruments\TNG\Stellar\1.0\System\Tools\ThermoPRMBuilderExternalTool.zip. This file can be installed in Skyline with Tools / External Tools and selecting this file with the Add button.

• Supplementary Data

  • Figures that support the main text
  • A derivation of the 2.547 conversion between FWHM and Base Width for Gaussian peaks

• LC/MS Information

  • Screen shots of the Vanquish Neo LC settings and method editor settings for the tMSn methods.
  • Note that we embed the instrument methods into the raw file, so they can always be recovered if the raw file is present. The information can be recovered in two ways:

• If you have a computer with the method editor installed, including the so-called Workstation builds that just have method editor, you can open the program here: C:\Program Files\Thermo Scientific\Instruments\TNG\Stellar\1.0\System\Programs\TNGMethodEditor.exe.

• The other way is more general, but doesn't give you an actual .meth file. If you open up Freestyle, and click the Reports / Instrument Method button, a pane will open up on the bottom. You can close other panes and press the instrument model name, Stellar in this case, to view the method details.