System Suitability Mix

• Pierce Retention Time Calibration (PRTC) stock is 5 pmol/ul and 200 ul total volume
• https://www.thermofisher.com/order/catalog/product/88321#/88321
• 15 heavy labeled peptides (see “PRTC_Manual_Thermo” PDF)
• BSA tryptic digest of ~ 2 ug/ul
• Two peptides that we look for – LVNELTEK and ATEEQLK

• For 500 ul of System Suitability mix (PRTC and BSA)
  • Add 20 ul of 5 pmol/ul PRTC stock for 200 fmol/ul
  • Add 15 ul 2 ug/ul BSA stock for 800 fmol/ul
  • Add 465 ul 0.1% formic acid
  • Aliquots are 10 ul (50 tubes) stored in -80 C
  • Dilute System Suitability mix with 30 ul 0.1% formic acid before analysis for 50 fmol/ul PRTC
  • Inject 6 ul total volume on LC for 300 fmol per System Suitability mass spec run