Mouse Skeletal Muscle Sarcopenia System Suitability
Mouse Skeletal Muscle Sarcopenia System Suitability
System Suitability Mix
- Pierce Retention Time Calibration (PRTC) stock is 5 pmol/ul and 200 ul total volume
- BSA tryptic digest of ~ 2 ug/ul
- Two peptides that we look for – LVNELTEK and ATEEQLK
- For 500 ul of System Suitability mix (PRTC and BSA)
- Add 20 ul of 5 pmol/ul PRTC stock for 200 fmol/ul
- Add 15 ul 2 ug/ul BSA stock for 800 fmol/ul
- Add 465 ul 0.1% formic acid
- Aliquots are 10 ul (50 tubes) stored in -80 C
- Dilute System Suitability mix with 30 ul 0.1% formic acid before analysis for 50 fmol/ul PRTC
- Inject 6 ul total volume on LC for 300 fmol per System Suitability mass spec run