System Suitability

System Suitability Mix

 

  • For 500 ul of System Suitability mix (PRTC and BSA)
    • Add 20 ul of 5 pmol/ul PRTC stock for 200 fmol/ul
    • Add 15 ul 2 ug/ul BSA stock for 800 fmol/ul
    • Add 465 ul 0.1% formic acid
    • Aliquots are 10 ul (50 tubes) stored in -80 C
    • Dilute System Suitability mix with 30 ul 0.1% formic acid before analysis for 50 fmol/ul PRTC
    • Inject 6 ul total volume on LC for 300 fmol per System Suitability mass spec run