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System Suitability
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Mouse Skeletal Muscle Sarcopenia System Suitability
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System Suitability Mix
Pierce Retention Time Calibration (PRTC) stock is 5 pmol/ul and 200 ul total volume
https://www.thermofisher.com/order/catalog/product/88321#/88321
15 heavy labeled peptides (see “PRTC_Manual_Thermo” PDF)
BSA tryptic digest of ~ 2 ug/ul
Two peptides that we look for – LVNELTEK and ATEEQLK
For 500 ul of System Suitability mix (PRTC and BSA)
Add 20 ul of 5 pmol/ul PRTC stock for 200 fmol/ul
Add 15 ul 2 ug/ul BSA stock for 800 fmol/ul
Add 465 ul 0.1% formic acid
Aliquots are 10 ul (50 tubes) stored in -80 C
Dilute System Suitability mix with 30 ul 0.1% formic acid before analysis for 50 fmol/ul PRTC
Inject 6 ul total volume on LC for 300 fmol per System Suitability mass spec run