Experimental design of in cellulo kinase assay

Warscheid Lab - Targeted FLNc S2233 S2236 phosphorylation assay



Cell-based kinase assay. PKC activator PMA, PKCa inhibitor Gö6976 and Akt activator IGF-1 and inhibitor MK-2206 were used to activate or block signaling pathways in C2 cells. For targeted MS analysis, phosphopeptides were enriched by Myc-tag and TiO2-based enrichment.