S2625 is the specific substrate site of PKCα in the hinge 2 region of mFLNC.

Warscheid Lab - C-terminal FLNc in vivo kinase assays

Figure 4 G-H, Experimental approach of quantitative in vivo kinase assay in contracting myotubes (G) and results for the regulated

peptide, phosphorylated at S2625 (H). Quantification of data shown was performed with skyline for n=3 independent experiments.

Shown is the mean log2 ratio of the area under the curve ± SEM.  **p ≤0.006.