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2024-04-30
S2625 is the specific substrate site of PKCĪ± in the hinge 2 region of mFLNC.
Extracted ion chromatogram of the mouse FLNc phosphopepetide GApSYSSIPK

S2625 is the specific substrate site of PKCĪ± in the hinge 2 region of mFLNC.


Figure 4 G-H, Experimental approach of quantitative in vivo kinase assay in contracting myotubes (G) and results for the regulated

peptide, phosphorylated at S2625 (H). Quantification of data shown was performed with skyline for n=3 independent experiments.

Shown is the mean log2 ratio of the area under the curve ± SEM.  **p ≤0.006.




Extracted ion chromatogram of the mouse FLNc phosphopepetide GApSYSSIPK


Figure S3C, Extracted ion chromatogram of the phosphopeptide GApSYSSIPK within

fraction 1 in the biological replicate 3 of the in vivo kinase assay.