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2+ charge state not available for selection

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2+ charge state not available for selection ss  2024-03-21 03:46:09
 

Hello,

I am building autoQC Skyline file and would like to select a 2+ precursor for one of the QC peptides but this is not availale in the SKyline precusor selection three (see attached). Other peptides in this list have the 2+ option available as 2+ is also listed in the transitions settings filter tab.

Thanks for you assistance.
 
 
Brendan MacLean responded:  2024-03-21 07:18:01

This is really a Skyline question. Please post to the Skyline support board. It would help if you also supplied screenshots of your Transition Settings - Instrument and Full-Scan tabs as well.

Thanks for you interest in AutoQC.
 
Nick Shulman responded:  2024-03-21 08:56:00

When you post your question on the Skyline support board, it would be helpful if you could include your Skyline document.
In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms and spectral libraries.

If that .zip file is less than 50MB you can attach it to your Skyline support request.
You can upload larger files here:
https://skyline.ms/files.url

-- Nick