Errror: Aminoacid value

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Errror: Aminoacid value ferdinando cerciello  2020-03-30 14:43:09
 

Hi all,
I m trying to upload my files on panorama, but when submitting the import button, I get this error back: AminoAcid: value is too long for column "AminoAcid", a maximum length of 1 is allowed. Supplied value was 4 characters long. I can t understand to what it refers. I tried by shortening the name of the files, but it did not help. The aminoacids within the files are normally visible within the Skyline file.

Thank you
Ferdinando

 
 
vsharma responded:  2020-03-30 17:47:15

Hi Ferdinando,

This looks like a bug in Panorama. You've entered an isotope modification ("Heavy") with two amino acids (K and R) as the targets. Panorama is expecting only one amino acid and that is why you are seeing the error. We will need to fix this to allow for more than one amino acid target for an isotope modification.

In the mean time, instead of using the custom "Heavy" isotope modification you could use the Unimod modifications already available in Skyline. When you add a new modification, click the arrow next to the "Name" field, in the "Edit Isotope Modification" dialog to see a list of the available modifications. The two corresponding to your "Heavy" modification are:
Label:13C(6)15N(2) (C-term K)
Label:13C(6)15N(4) (C-term R)

Using these two modifications should fix the Panorama upload problem.

Best regards,
Vagisha

 
ferdinando cerciello responded:  2020-03-31 13:23:22

Thank you Vagisha,

this was very helpful and it works so far!

Best regards,
Ferdinando