Unable to upload Skyline file

Unable to upload Skyline file Andreas Hober  2019-03-19

I've been trying to upload a Skyline file to one of my Panorama projects without any success.
I've tried uploading it through Skyline, as well as directly uploading the zipped Skyline file through the web interface, but neither works.
I get an error message in the Data Pipeline stating "No transition match!".

The particular data set is due for submission and any help with managing to upload the data would be much appreciated.

Best regards

vsharma responded:  2019-03-19

Hi Andreas,

The problem is with transition y7 for the precursor 612.3215++ (heavy), peptide ATEHLSTLSEK[+8.0]. There is no matching chromatogram for this transition in Skyline's .skyd file. If you remove this transition the file will import on Panorama. You could also try to re-import the results: Edit > Manage Results > Re-import button. I think this would also fix the problem. Let me know if it does not.