Figure 1 Cell Fitness, stress response, and miscoding of Str-treated wt and non-treated error-prone E. coli strains

(B) Correlation between misreading and proteotoxic stress response for Str-treated wt cells (black) and untreated ram cells (blue) circles. The average of three median error frequencies is plotted; error bars represent the SEM of three biological replicates.

(C) Misreading profiles of wt Str-treated and ram cells (shown are the data for 6 µM Str; see also Figure S1G). Str-specific amino acid substitutions are shown in warm colors; amino acid substitutions specific for ram strain in cold colors.

Figure S1 Changes in the cellular proteome of cells upon Str-treatment. Related to Figure 1

(G) Error frequencies in Str-treated and ram cells. Single amino acid substitution in the model protein EF-Tu were quantified by mass spectrometry using cell lysates described in (A). Gray points represent individual peptides. For each condition, median error frequencies are indicated by dashed lines. Note that due to the low error frequency of most amino acid substitutions and the dynamic range restrictions of the mass spectrometer, the median error frequencies do not reflect the global median error frequency of near-cognate misreading, but are solely used for relative comparison of miscoding in wt Str-treated vs. ram cells.

Wild type cells are named B209 (Str-treated and untreated)

Ram cells (ribosome ambiguity) are named B212 (untreated)

For details please see the method section and Table S1.

RAW files, related RAW files for streptomycin-induced changes in the proteome and a table with spectral evidence for error clusters was submitted to the Proteome exchange repository.