| QC Measure | Description |
|---|---|
| System suitability | PRM injections used for nearly real-time data assessment at the beginning, middle, and end of run sequence. Also used for longitudinal tracking of a system in conjunction with digital notebooks, Skyline, and Panorama AutoQC. |
| Inter-batch external QC sample |
External quality control sample that is prepared alongside each batch of samples to correct for batch-to-batch sample preparation and data collection differences in intensity. Similar composition, but different pool than the Inter-experiment quality control. Generated from samples in an individual sample batch. |
| Inter-experiment external QC sample | External quality control sample to assess computational and statistical strategies after adjusting for run order, batch effects, calibration, etc. Prepared alongside each batch of samples. Similar composition, but different pool than the Inter-batch quality control. Can be prepared from analytical samples, or from samples of the same or similar matrix. |
| Peptide internal quality control | Internal quality control. Peptides spiked into samples to monitor system function. When used in conjunction with protein internal quality controls, can be used to parse out whether the process is the root of an identified issue. |
| Protein internal quality control | Internal quality control. Protein spiked into sample to assess sample processing and peptide recovery. May examine digestion or PTM enrichment. |
| QC Measure | Citations |
|---|---|
|
System suitability |
|
|
Internal quality controls + System Suitability |
|
|
Internal quality controls + External quality controls + System Suitability |