Degradation kinetics of lignocellulolytic enzymes in a biogas reactor by quanti-tative mass spectrometry
Küchler, J.; Willenbücher, K.; Reiß, E.; Nuß, L.; Conrady, M.; Ramm, P.; Schimpf, U.; Reichl, U.; Szewzyk, U.; Benndorf, D. Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry. Fermentation 2023, 9, 67. https://doi.org/10.3390/fermentation9010067
- Organism: Lentinula edodes
- Instrument: timsTOF Pro
Mass spectrometry, Biogas production, Fungal enzymes, lignocellulose conversion, Lentinula edodes
Submitter: Jan Küchler
Supplementation of lignocellulose-degrading enzymes can be used to enhance the performance of biogas production in industrial biogas plants. Since the structural stability of these enzyme prepa-rations is essential for efficient application, reliable methods for the assessment of enzyme stability are crucial. Here, a mass spectrometric-based assay was established to monitor the structural sta-bility of enzymes, i.e. the structural integrity of these proteins, in anaerobic digestion (AD). Analy-sis of extracts of Lentinula edodes revealed rapid degradation of lignocellulose-degrading enzymes with an approximate half-life of 1.5 h.
The observed low structural stability of lignocellulose-degrading enzymes in AD corresponded with previous results obtained for biogas content. The established workflow can be easily adapted for the monitoring of other enzyme formulations and provides a platform for evaluating the effect of enzyme additions in AD together with a characterization of the biochemical methane potential used to determine the biodegradability of organic substrates.
Addition of lignocellullolytic enzyme mixture derived from Lentinula edodes to biogas sample;
measuring of degradation kinetics of lignocellulolytic enzymes by detecting signature peptides via DIA and DDA mass spectrometry
biogas samples, lentinula edodes
Created on 1/18/23, 8:56 PM