U of Leipzig AG Bioanalytics - Der p in SEA

In-Gel digest and filter-aided sample preparation to identify Dermatophagoides - and yeast protein antigens in severe equine asthma.
Data License: CC BY 4.0 | ProteomeXchange: PXD045568 | doi: https://doi.org/10.6069/pfd5-zf32
  • Organism: Saccharomyces cerevisiae, Dermatophagoides pteronyssinus
  • Instrument: SYNAPT G2-Si
  • SpikeIn: No
  • Keywords: Immunoglobulin isotypes, proteomics, 2D immunoblot, LC-MS, asthma, COPD, RAO, heaves
  • Lab head: Ralf Hoffmann Submitter: Daniela Volke
Severe equine asthma (SEA) is a common, chronic respiratory disease of horses characterized by hyperreactivity to hay dust which has many similarities to severe neutrophilic asthma in humans. A 2D immunoblot approach was used to identify quantitative differences in the recognition of the two-dimensional gel electrophoresis (2-DE) pattern between asthmatic and helthy horses´sera. Differentially regulated spots were cut from a 2-D gel and used for in-gel digestion followed by LC-MS to identify possible antigens.
Experiment Description
Peptides obtained after tryptic in-gel digest or FASP were analyzed with a nanoACQUITY Ultra Performance LC™ coupled to a Q-TOF SYNAPT G2-Si instrument. For measurments a high-definition data-dependent acquisition approach (HD-DDA) for top 6 ions was used. Followed by data analysis with Mascot Distiller (Version and Mascot search engine (Version 2.7.0).
Sample Description
103 tryptic in-gel digested samples, 1 tryptic filter-aided sample preparation sample (100 ng on column)
Created on 9/21/23, 11:18 AM

Excel files contain the database results filted for proteins with 3 peptides per protein and a Mascot score of 50. In-Gel digest files contain pictures of the cutted 2 gels and gives an overview which .raw file belongs to which spot, mgf or dat file.

  Attached Files  
 FASP_Der p.xlsx

Clustergrammer Heatmap
InGelDigest_Der_p_SEA_II_2023-09-18_10-01-52.sky.zip2023-09-21 11:03:445004,0584,42026,52051
InGelDigest_Der_p_SEA_I_2023-09-15_16-14-11.sky.zip2023-09-21 11:03:443272,3822,52115,12652
FASP_Der_p_SEA_2023-09-15_14-24-26.sky.zip2023-09-21 11:03:445504,2964,77128,6261