Description- This study provides targeted verification of proteins from the anterior and posterior lobe of Pituitary glands. A BiblioSpec library was generated in Skyline from Proteome Discoverer results containing 5 anterior and 5 posterior lobes of pituitary gland. Our LFQ data is available with the identifier PXD030235 via the PRIDE member repository of the ProteomeXchange Consortium. The data uploaded to PRIDE consists of anterior and posterior raw files generated from orbitrap, mgf peak file, msf search file, fasta sequence used and others containing PD result file. The raw files used to build the BiblioSpec library were renamed prior to uploading to PRIDE. The table below provides a mapping of the original raw file names with files on PRIDE.