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IIT Bombay Proteomics - Anterior vs Posterior Pituitary PRM

Targeted MS Experiment

 
Proteomic Blueprint of the Pituitary anterior and posterior lobes for better understanding of the physiology of pituitary gland.
Permanent Link: https://panoramaweb.org/antpstpituitary.url Share
Banerjee A, Biswas D, Barpanda A, Halder A, Sibal S, Kattimani R, Shah A, Mahadevan A, Goel A, Srivastava S. The first Pituitary Proteome Landscape from matched anterior and posterior lobes for a better understanding of the Pituitary Gland. Mol Cell Proteomics. 2022 Dec 2:100478. doi: 10.1016/j.mcpro.2022.100478. Epub ahead of print. PMID: 36470533.
Data License: CC BY 4.0 | ProteomeXchange: PXD029991 | doi: https://doi.org/10.6069/z8b7-g295
  • Organism: Homo sapiens
  • Instrument: Orbitrap Fusion
  • SpikeIn: No
  • Keywords: proteomics, PRM, Fusion
  • Lab head: Sanjeeva Srivastava Submitter: Sanjeeva Srivastava
Abstract
This study provides a comprehensive proteomic analysis of five matched pairs of the anterior and posterior lobe of the pituitary, which was used to study the proteomic signature specific to both anterior and posterior lobes.
Experiment Description
Pituitary matched anterior and posterior lobes were lysed using lysis buffer (8M Urea, 50 mM Tris pH 8.0, 75 mM NaCl, 1mM MgCl2) supplemented with protease inhibitors. The homogenates were centrifuged to obtain the supernatant. Proteins in the supernatant were quantified with BCA protein quantification. Protein enzymatic cleavage was carried out with trypsin at 37 °C for 16 h. Peptides were desalted and vacuum dried. Further, these peptides were quantified using the Scopes method. After quantification, these peptides were subjected to Lable free quantification using nLC-Orbitrap Mass Spectrometric techniques.
Created on 12/15/21, 5:08 PM

Targeted MS Runs

 
Clustergrammer Heatmap
 
Download
101021_AB_AntvsPos_PRM for pprV1_2021-11-25_15-32-30.sky.zip
291 MB 
2021-12-15 17:08:181332322046

Mass Spec Search

 

Targeted Verification of proteins from Anterior and posterior pituitary lobes

 

Description- This study provides targeted verification of proteins from the anterior and posterior lobe of Pituitary glands. A BiblioSpec library was generated in Skyline from Proteome Discoverer results containing 5 anterior and 5 posterior lobes of pituitary gland. Our LFQ data is available with the identifier PXD030235 via the PRIDE member repository of the ProteomeXchange Consortium.  The data uploaded to PRIDE consists of anterior and posterior raw files generated from orbitrap, mgf peak file, msf search file, fasta sequence used and others containing PD result file. The raw files used to build the BiblioSpec library were renamed prior to uploading to PRIDE. The table below provides a mapping of the original raw file names with files on PRIDE.

Original ID PRIDE Renaming
02022020_Fusion_1049_AB_A459.raw A459.raw
02022020_Fusion_1049_AB_A460.raw A460.raw
16022020_Fusion_AB_1049_A441.raw A441.raw
16022020_Fusion_AB_1049_A442.raw A442.raw
16022020_Fusion_AB_1049_A457.raw A457.raw
27062020_AB_PT442.raw PT442.raw
27062020_AB_PT457.raw PT457.raw
27062020_AB_PT459.raw PT459.raw
29062020_AB_PT441.raw PT441.raw
29062020_AB_PT460.raw PT460.raw