Utrecht U Targeted Proteomics - vanGelder_et_al._2020

Clustergrammer Heatmap
vanGelder_Kinase_phosphorylation_2020-07-30_16-35-41.sky.zip2020-08-06 12:03:144510502
Temporal quantitative proteomics of mGluR-induced protein translation and phosphorylation in neurons

  • Organism: Rattus norvegicus
  • Instrument: TSQ Altis
  • SpikeIn: Yes
  • Keywords: mGluR-LTD, proteomics, phosphoproteomics, synaptic plasticity, protein translation, AMPA receptor, bioorthogonal amino acid tagging, metabolic labeling, tandem mass tags
At neuronal synapses, activation of group I metabotropic glutamate receptors (mGluR1/5) triggers a form of long-term depression (mGluR-LTD) that relies on new protein synthesis and the internalization of AMPA-type glutamate receptors. Dysregulation of these processes has been implicated in the development of mental disorders such as autism spectrum disorders and therefore merit a better understanding on a molecular level. Here, to study mGluR-induced signaling pathways, we integrated quantitative phosphoproteomics with the analyses of newly synthesized proteins via bio-orthogonal amino acids (azidohomoalanine) in a pulsed labeling strategy in cultured hippocampal neurons stimulated with DHPG, a specific agonist for group I mGluRs. We identified several kinases with important roles in DHPG-induced mGluR activation, which we confirmed using small molecule kinase inhibitors. Furthermore, changes in the AMPA receptor endocytosis pathway in both protein synthesis and protein phosphorylation were identified, whereby Intersectin-1 was validated as a novel player in this pathway. This study revealed several new insights into the molecular pathways downstream of group I mGluR activation in hippocampal neurons, and provides a rich resource for further analyses.
Created on 8/6/20, 12:03 PM