In this experiment we monitored 34 peptides, belonging to 12 proteins that were found to be over-expressed in COVID severe vs non severe from the lab's LFQ data. We ran 12 (6 severe and 6 non severe) COVID plasma samples in replicates on a triple quadrupole mass spectrometer against this list of 35 peptides (including one spiked in heavy labelled synthetic peptide to monitor run to run response). Only peptides that showed differential regulation in the MRM data were retained in the final Skyline files. We also created a library using the shotgun data and further refined the transition list. The submitted Skyline file has this refined list of 29 peptides and 183 transitions. The two bio-replicates of the same sample ID were run on two different days to check reproducibility.