Figure 4B. 

In vitro kinase assay coupled to quantitative MS analysis for site determination. Reactions were performed as described in figure 4A. MS data for each kinase were quantified using Skyline and are available in this Panorama project. Intensities of phosphopeptides distinctive for a specific phosphorylation site in hFLNc d18-21 WT were added up per experiment and represented as normalized mean ± SEM (n=3).