| | | | | | | |
| Calibrated mass axis.
positive mode passed.
negative mode passed.
LBC | 2022-09-14 | | Instrumentation Change | Exploris_Dabney | 2022-09-14 | 2022-09-14 |
| Changed trap and anlytical columns.
Acclaim PepMap 100: SN 20349855
PepMap RSLC C18: SN C20298282
LBC | 2022-09-14 | | nanoLC Column | Exploris_Dabney | 2022-09-14 | 2022-09-14 |
| Full calibration performed by Leonard Collins.
positive mode passed.
Negative mode passed.
Set up Easy nLC-1200 for system evaluation.
LBC | 2022-09-12 | | Instrumentation Change | Exploris_Dabney | 2022-09-13 | 2022-09-13 |
| Full cleaning performed by Leonard Collins.
Cleaned ion funnel, entrance quad, and isolation quad.
White residue on all 4 rods of isolation quad that required hard wiping with wet cloth.
LBC | 2022-09-08 | | Instrumentation Change | Exploris_Dabney | 2022-09-13 | 2022-09-13 |
| Mass axis calibration
positive mode: passed
negative mode: passed
LBC | 2022-03-07 | | Instrumentation Change | Exploris_Dabney | 2022-03-07 | 2022-03-07 |
| Mass axis calibration
positive mode: passed
negative mode: passed
LBC | 2022-02-24 | | Instrumentation Change | Exploris_Dabney | 2022-02-24 | 2022-02-24 |
| Mass and system calibration (Pos and Neg)
Dab 480
YCC | 2022-02-16 | | Instrumentation Change | Exploris_Dabney | 2022-02-18 | 2022-02-18 |
| Analytical column changed | 2022-01-17 | | nanoLC Column | Exploris_Dabney | 2022-01-20 | 2022-01-20 |
| Full calibration:
positive mode: passed
Mass calibration:
negative mode: passed
Analytical column was plugged. Sonicated for a very long time. It "might"be fixed. Need to run HeLa.
LBC | 2021-12-29 | | Instrumentation Change | Exploris_Dabney | 2021-12-29 | 2021-12-29 |
| Because the flow was shut off and the column not properly prepped for storage during the time it took for Thermo to make the pump repair, the analytical column became plugged, likely due to evaporation at the tip leaving an insoluble deposit. We should create a short procedure to flush the column before we have Thermo engineers make repairs, since we do not know exactly how long the downtime will be.
New Trap: SN 20229892
New column: SN C20294372
LBC | 2021-12-10 | | nanoLC Column | Exploris_Dabney | 2021-12-10 | 2021-12-10 |
| Full calibration
positive mode: passed
negative mode: passed
LBC | 2021-12-08 | | Instrumentation Change | Exploris_Dabney | 2021-12-10 | 2021-12-10 |
| Oil rough pumps removed.
Dry rough pump installed: nXL 110i Dual Inlet
John Polese | 2021-12-07 | | Thermo Scientific | Exploris_Dabney | 2021-12-08 | 2021-12-10 |
| Engineer replaced Pump B on the easyNano.
LBC | 2021-12-06 | | Thermo Scientific | Exploris_Dabney | 2021-12-10 | 2021-12-10 |
| Trap column changed SN 202229866 | 2021-11-16 | | nanoLC Column | Exploris_Dabney | 2021-11-16 | 2021-11-16 |
| Mass axis calibration:
positive mode passed
negative mode passed
LBC | 2021-10-28 | | Instrumentation Change | Exploris_Dabney | 2021-10-28 | 2021-10-28 |
| Fresh the solvents | 2021-10-22 | | Reagent Change | Exploris_Dabney | 2021-11-16 | 2021-11-16 |
| Both analytical and trap columns are changed.
Analytical SN C20216694
Trap SN 20229891
HB | 2021-10-14 | | nanoLC Column | Exploris_Dabney | 2021-11-16 | 2021-11-16 |
| MPA and MPB were refreshed in the nanoLC.
--Taufika Islam Williams | 2021-09-17 | | Reagent Change | Exploris_Dabney | 2021-09-23 | 2021-09-23 |
| Mass axis calibration:
positive mode passed
negative mode passed
LBC | 2021-09-17 | | Instrumentation Change | Exploris_Dabney | 2021-09-17 | 2021-09-17 |
| Mass axis calibration:
positive mode passed
negative mode passed
LBC | 2021-09-13 | | Instrumentation Change | Exploris_Dabney | 2021-09-13 | 2021-09-13 |
| Full calibration:
positive mode passed
negative mode passed | 2021-08-24 | | Instrumentation Change | Exploris_Dabney | 2021-08-24 | 2021-08-24 |
| Mass Calibration | 2021-08-20 | | Instrumentation Change | Exploris_Dabney | 2021-08-20 | 2021-08-20 |
| Easy-Spray temperature control cable was replaced today.
Production Order: 0247632.
LC167 Revision B.
Jon Delgado from Thermo was onsite and looked at it. He will order a new one for us.
--TIW | 2021-07-27 | | Instrumentation Change | Exploris_Dabney | 2021-07-27 | 2021-07-27 |
| Mass Calibration: +ve and -ve.
--Taufika Islam Williams | 2021-07-27 | | Instrumentation Change | Exploris_Dabney | 2021-07-27 | 2021-07-27 |
| Full calibration.
positive mode: passed
negative mode: passed
LBC | 2021-07-22 | | Instrumentation Change | Exploris_Dabney | 2021-07-23 | 2021-07-23 |
| Trap column replaced.
Acclaim Pepmap 100, 75um x 2 cm
SN 20229892 | 2021-07-12 | | nanoLC Column | Exploris_Dabney | 2021-07-12 | 2021-07-12 |
| Mass axis calibration:
positive: passed
negative: passed
LBC | 2021-07-12 | | Instrumentation Change | Exploris_Dabney | 2021-07-12 | 2021-07-12 |
| Mass Calibration +ve and -ve.
--TIW | 2021-07-02 | | Instrumentation Change | Exploris_Dabney | 2021-07-27 | 2021-07-27 |
| Quad clean.
Full calibration.
-Jon Delgado (FSE) | 2021-06-23 | | Thermo Scientific | Exploris_Dabney | 2021-06-25 | 2021-06-25 |
| I swapped computers and managed to swap the d drives so all your data is in the new computer.
I upgraded you all to Tune 3.0 and set the vnc connection for the EasyNano which is actively working.
I'll take back the old computer to RMA it.
You all will have to set up your IT to come in and map this to your network so you all can remote in.
I'm currently running through system checks and will give you another update in an hour or so.
Instrument passed both positive and negative checks and I see no issues with the instrument. When one of you has a moment, could we start an injection just to make sure there are no issues with the MS triggering?
Jon Delgado
Field Service Engineer III | 2021-06-07 | | Thermo Scientific | Exploris_Dabney | 2021-06-07 | 2021-06-07 |
| Alllison Stewart opened a ticket and will let us know when an IP address has been assigned to the new computer.
Computer name: JZ3NB93; User name: MA10095N; PWD: Thermo.
Once the new computer has internet access, we will start a QC sequence.
DinoLite software will also need to be installed.
--TIW | 2021-06-07 | | Instrumentation Change | Exploris_Dabney | 2021-06-07 | 2021-06-07 |
| Full calibration of MS (+ve & -ve)
Clean HCTT.
--TIW | 2021-04-15 | | Instrumentation Change | Exploris_Dabney | 2021-04-15 | 2021-04-15 |
| New trap column
New analytical column.
--TIW | 2021-04-15 | | nanoLC Column | Exploris_Dabney | 2021-04-15 | 2021-04-15 |
| Fresh nanoLC solvents (MPA & MPB)
Performed leak test
Checked flow sensor calibration.
--TIW | 2021-04-15 | | Reagent Change | Exploris_Dabney | 2021-04-15 | 2021-04-15 |
| Mass calibration: positive
Mass calibration: negative
LBC | 2021-04-09 | | Instrumentation Change | Exploris_Dabney | 2021-04-09 | 2021-04-09 |
| Mass calibration (positive and negative). | 2021-03-29 | | Instrumentation Change | Exploris_Dabney | 2021-03-29 | 2021-03-29 |
| Prepared and changed fresh solvent | 2021-03-16 | | Reagent Change | Exploris_Dabney | 2021-04-05 | 2021-04-05 |
| (1) HCTT cleaned (2) Mass calibration. | 2021-03-15 | | Instrumentation Change | Exploris_Dabney | 2021-03-15 | 2021-03-15 |
| Prepared fresh nanoLC solvents. | 2021-03-15 | | Reagent Change | Exploris_Dabney | 2021-03-15 | 2021-03-15 |
| New trap column:
SN 10887042
LBC and HB | 2021-02-02 | | nanoLC Column | Exploris_Dabney | 2021-02-02 | 2021-02-02 |
| Full calibration negative mode.
Mass calibration positive mode.
LBC and HB | 2021-02-02 | | Instrumentation Change | Exploris_Dabney | 2021-02-02 | 2021-02-02 |
| Training with Hellena Bai.
New trap column: SN 20087104
Analytical column: SN B20144245 | 2021-01-11 | | Instrumentation Change | Exploris_Dabney | 2021-01-11 | 2021-01-11 |
| Annual PM performed. John Polese. | 2021-01-08 | | Thermo Scientific | Exploris_Dabney | 2021-01-11 | 2021-01-11 |
| Analytical column replaced
SN C20290940 | 2021-01-07 | | nanoLC Column | Exploris_Dabney | 2022-01-17 | 2022-01-17 |
| Full Calibration performed. | 2020-12-15 | | Instrumentation Change | Exploris_Dabney | 2020-12-15 | 2020-12-15 |
| Glycomics Study (METRIC-MS Order # 100098878).
PI: David Muddiman
Graduate Student: Jackie K.
Nov 07 - Nov 11. | 2020-11-07 | | METRIC User Change | Exploris_Dabney | 2020-11-16 | 2020-11-16 |
| (1) HCTT cleaned (2) Full calibration. | 2020-11-06 | | Instrumentation Change | Exploris_Dabney | 2020-11-16 | 2020-11-16 |
| Mobile phases topped off. Wash reservoirs also topped off. LBC | 2020-10-18 | | Reagent Change | Exploris_Dabney | 2020-10-16 | 2020-10-16 |
| Trap column changed. Installed SN 10887043.
Removed SN 10887044. LBC | 2020-10-18 | | nanoLC Column | Exploris_Dabney | 2020-10-16 | 2020-10-16 |
| Analytical column changed. Installed SN 10613732, 75 cm, for Kleiner.
Removed SN 10816988, 25 cm. LBC | 2020-10-18 | | nanoLC Column | Exploris_Dabney | 2020-10-16 | 2020-10-16 |
| Installed column for Manuel Kleiner
75 um x 75 cm | 2020-09-21 | | nanoLC Column | Exploris_Dabney | 2020-09-21 | 2020-09-21 |
| Instrument Control Board Failure
FSE: John Polese (john.polese@thermofisher.com)
Sep 12 - Sep 18, 2020
Thermo Ticket # 330996
Serial Numbers: Exploris 480, MA10095N
Easy nLC-1200, LC-030956
Issues: - Switch Box and Instrument Control PCB.
Note from John P. (FSE): Your instrument is functioning again. It was a matter of resetting a particular board that doesn't reset under normal methods. Regardless, the instrument is ready for use again. | 2020-09-12 | | Thermo Scientific | Exploris_Dabney | 2020-09-15 | 2020-09-21 |
| Issue with quads.
FSE: John Polese (john.polese@thermofisher.com)
Jul 23 - Aug 07, 2020
Thermo Ticket # 293232
Serial Numbers: Exploris 480, MA10095N
Easy nLC-1200, LC-030956
-It is a known issue and we were told to continue using the instrument until the part was available for installation.
From John P.: After careful review of your instrument logs the issue appears to have corrected itself. The high voltage condition existed for a very short duration between 5am - 1030am on July 23. In all the records and all the runs you have done since then the voltage has remained rock solid where it should be. As such, I see no justification in replacing the quad PCB at this time: It's been about 2 weeks of in place testing and has proven itself functional. I will forward these logs back to the factory for their troubleshooting process, but this system is operating nominally. No modification to your instrument were made, it is yours to run with. | 2020-08-23 | | Thermo Scientific | Exploris_Dabney | 2020-09-15 | 2020-09-15 |
| Taufika begins MSB-Duke-ALS study.
METRIC Order # 100085754.
Aug 20 - 25, 2020. | 2020-08-20 | | METRIC User Change | Exploris_Dabney | 2020-09-15 | 2020-09-15 |
| Changed mobile phases and wash solvents. Mobile phases were prepared by LBC on 5/26/20.
Sonicated column tip.
Sonicated heated transfer line.
Performed full calibration. | 2020-08-14 | | Reagent Change | Exploris_Dabney | 2020-08-14 | 2020-08-14 |
| Column SN B20091050 clogged while waiting for instrument service.
Replaced with new column SN 20087122.
Leonard Collins | 2020-07-20 | | nanoLC Column | Exploris_Dabney | 2020-07-23 | 2020-07-23 |
| HCD Cell Error.
FSE: John Polese (john.polese@thermofisher.com)
Jul 01 - 21, 2020
Thermo Ticket # 278344.
Serial Numbers: Exploris 480, MA10095N
Easy nLC-1200, LC-030956
-The main issue was spray stability. The neg mode is particularly difficult to stabilize on this instrument right now, and John was not terribly satisfied with what he ended up with. But it got us through the calibrations.
-John would recommend replacing the low flow needle in the ESI probe at your earliest convenience.
-Otherwise, with the update to tune 2.0, the instrument behaved very well.
----------------
Issues:
-Every few runs, we see a RED error message on the Tune Page and the instrument stalls. The error seems to be associated with the HCD cell. We have checked our gas tank and it is ok. In previous communications, I have sent you screenshots of the error. A couple are attached here.
-We have also observed some communication issues between the nanoLC and the MS.
-Additionally, we can no longer use TightVNC to 'see' the nanoLC interface on the instrument computer screen (despite multiple computer restarts). This is problematic for us to manage experiments remotely from home. | 2020-07-01 | | Thermo Scientific | Exploris_Dabney | 2020-09-15 | 2020-09-15 |
| Taufika begins Menegatti-Carbonell NIIMBL study
METRIC Order # 100076188.
Jun 01 - Jul 30, 2020. | 2020-06-01 | | nanoLC Column | Exploris_Dabney | 2020-09-15 | 2020-09-15 |
| nanoLC-1200
Analytical Column: ES802A; 75 um x 25 cm; 2 um, 100A
Trap: P/N 164946; Acclaim PepMap 100; 75 um x 2 cm
MPA: 98% water, 2% ACN, 0.1% HCOOH
MPB: 80% ACN, 20% water, 0.1% HCOOH | 2020-02-07 | | nanoLC Column | Exploris_Dabney | 2020-09-15 | 2020-09-15 |
| Taufika begins Reading Striped Bass Project Phase II.
METRIC Order # 100067868.
Feb 01 - Mar 30, 2020. | 2020-02-01 | | METRIC User Change | Exploris_Dabney | 2020-09-15 | 2020-09-15 |
| Jan 06-09: nanoLC-Exploris-480 training (Tahmid H.) | 2020-01-06 | | Instrumentation Change | Exploris_Dabney | 2020-09-15 | 2020-09-15 |
| nanoLC-1200-Exploris-480 familiarization. | 2019-12-12 | | Instrumentation Change | Exploris_Dabney | 2020-09-15 | 2020-09-15 |
| Installation of nanoLC-1200-Exploris-480 in DAB 28. | 2019-12-06 | | Instrumentation Change | Exploris_Dabney | 2020-09-15 | 2020-09-15 |
| ALL DATA BELOW THIS POINT WAS FOR THE POLK_nanoLC-1200-QE-HFX. ALL DATA AFTER THIS POINT IS FOR THE DAB_nanoLC-1200-Exploris-480. | 2019-12-01 | | Instrumentation Change | Exploris_Dabney | 2020-09-15 | 2020-09-15 |
| Oct 03-15, 2019
Taufika Islam Williams
MENEGATTI/CARBONELL PROJECT
24 sample injections.
nanoLC-1000
Analytical Column: ES802A; 75 um x 25 cm; 2 um, 100A (08/15/19) Trap: P/N 164946; Acclaim PepMap 100; 75 um x 2 cm (10/03/19)
MPA: 98% water, 2% ACN, 0.1% HCOOH
MPB: 98% ACN, 2% water, 0.1% HCOOH | 2019-10-03 | | METRIC User Change | Exploris_Dabney | 2019-10-03 | 2020-09-15 |
| Pump A on nanoLC-1200 maxed out in pressure.
The nanoLC-1000 was installed, while the troubleshooting was conducted on the nanoLC-1200. It so happened that the column installed on 09/20/19 became clogged after only running blanks and std. HeLa. The column from 08/16/19 was re-installed on the nanoLC-1000 with a brand new trap (10/02/19).
MPA: 98% water, 2% ACN, 0.1% HCOOH
MPB: 98% ACN, 2% water, 0.1% HCOOH | 2019-10-02 | | Instrumentation Change | Exploris_Dabney | 2019-10-03 | 2019-10-03 |
| New columns...
Analytical: 25 cm (2 µm particle size, 100 Å pore size, 75 µm diameter) PepMapTM C18 column (P/N ES802A)
Trap: 2 cm (3 µm particle size, 100 Å pore size, 75 µm diameter) Acclaim PepMapTM C18 column (P/N 164946)
...installed by TIW on the nanoLC-1200.
MPA: 98% water, 2% ACN, 0.1% HCOOH
MPB: 80% ACN, 20% water, 0.1% HCOOH | 2019-09-20 | | nanoLC Column | Exploris_Dabney | 2019-10-03 | 2019-10-03 |
| nanoLC-1200 installed by:
Joy Miller
Field Service Engineer
Mobile: 706-371-6096
www.unitylabservices.com | 2019-09-09 | | Instrumentation Change | Exploris_Dabney | 2019-10-03 | 2019-10-03 |
| Aug 17-28, 2019
Taufika Islam Williams
MENEGATTI/CARBONELL NIIMBL PROJECT
24 sample injections | 2019-08-17 | | METRIC User Change | Exploris_Dabney | 2019-10-03 | 2019-10-03 |
| New columns...
Analytical: 25 cm (2 µm particle size, 100 Å pore size, 75 µm diameter) PepMapTM C18 column (P/N ES802A)
Trap: 2 cm (3 µm particle size, 100 Å pore size, 75 µm diameter) Acclaim PepMapTM C18 column (P/N 164946)
...installed by TIW on nanoLC-1000. | 2019-08-16 | | nanoLC Column | Exploris_Dabney | 2019-08-19 | 2019-10-03 |
| Jul 11, 2019
Jon Delgado
Replaced: (1) split flow source turbo (2) Pfeiffer rough pump
FULL calibration on QEHFX | 2019-07-11 | | Thermo Scientific | Exploris_Dabney | 2019-07-19 | 2019-07-19 |
| Jul 03-06, 2019
Taufika Islam Williams
MENEGATTI/CARBONELL NIIMBL PROJECT
24 sample injections
GOSHE PROJECT
6 sample injections
MPA: 100% water, 0.1% HCOOH; MPB: 100% ACN, 0.1% HCOOH | 2019-07-03 | | METRIC User Change | Exploris_Dabney | 2019-07-19 | 2019-10-03 |
| Jun 17-18, 2019
Taufika Islam Williams
GOSHE PROJECT
18 sample injections | 2019-06-17 | | METRIC User Change | Exploris_Dabney | 2019-07-19 | 2019-07-19 |
| Apr 02 - May 24, 2019
Taufika Islam Williams
READING Striped Bass PROJECT Study Samples
FULL calibration on QEHFX
295 sample injections | 2019-04-02 | | METRIC User Change | Exploris_Dabney | 2019-07-19 | 2019-07-19 |
| April 01, 2019
Thermo nanoLC Column installed by Phil Loziuk (Thermo)
Analytical: 25 cm (2 µm particle size, 100 Å pore size, 75 µm diameter) PepMapTM C18 column (P/N ES802A)
Trap: 2 cm (3 µm particle size, 100 Å pore size, 75 µm diameter) Acclaim PepMapTM C18 column (P/N 164946)
FULL calibration on QEHFX | 2019-04-01 | | nanoLC Column | Exploris_Dabney | 2019-07-19 | 2019-07-19 |
| April 01, 2019
Phil Loziuk (Thermo)
Thermo EASY Spray nanoLC source installed | 2019-04-01 | | Instrumentation Change | Exploris_Dabney | 2019-07-19 | 2019-07-19 |
| Mar 11, 2019
Brian Fulp (Thermo FSE): clean front end and the quads.
FULL calibration on QEHFX | 2019-03-12 | | Thermo Scientific | Exploris_Dabney | 2019-07-19 | 2019-07-19 |
| Mar 07, 2019
Joy Miller (Thermo FSE): nanoLC repair (replacement of rotor seals and some lines including the syringe line) | 2019-03-07 | | Instrumentation Change | Exploris_Dabney | 2019-07-19 | 2019-07-19 |
| Feb 07-17, 2019
Taufika Islam Williams
WANG PROJECT
Analytical Column: created using 75 μm × 25 cm PicoFrit columns (New Objective, Woburn, MA) which were self-packed with ReproSil-Pur 120 Å C18-AQ 3.0 μm particles (Dr. Maisch, Ammerbuch, Germany).
Trap: 5 cm (constructed similarly)
First proteomics project on this instrument after move to Polk.
FULL calibration on QEHFX
63 sample injections | 2019-02-07 | | METRIC User Change | Exploris_Dabney | 2019-07-19 | 2019-07-19 |
| Dec 04 -17, 2018
Taufika Islam Williams
READING Striped Bass PROJECT Method Development
Analytical columns were created using 75 μm × 25 cm PicoFrit columns (New Objective, Woburn, MA) which were self-packed with ReproSil-Pur 120 Å C18-AQ 3.0 μm particles (Dr. Maisch, Ammerbuch, Germany).
Shortly after this project, we moved the nanoLC-1000/QEHFX to Polk. This is a temporary relocation, while renovations are completed in METRIC lab spaces in the basement of Dabney.
67 sample injections | 2018-12-04 | | METRIC User Change | Exploris_Dabney | 2019-07-19 | 2019-07-19 |
| Dec 01, 2018
nanoLC interface on QEHFX: Thermo nanoLC-1000 | 2018-12-01 | | Instrumentation Change | Exploris_Dabney | 2019-07-19 | 2019-07-19 |
| Dec 01, 2018
Mobile phases suitable for the Thermo nanoLC-1000
MPA: 100% water with 0.1% formic acid
MPB: 100% ACN with 0.1% formic acid | 2018-12-01 | | Reagent Change | Exploris_Dabney | 2019-07-19 | 2019-07-19 |
| Oct 18, 2018
Brian Fulp
FULL calibration on QEHFX | 2018-10-18 | | Thermo Scientific | Exploris_Dabney | 2019-07-19 | 2019-07-19 |
| April 09, 2018
Brian Fulp
New Instrument Installation
FULL calibration on QEHFX | 2018-04-09 | | Thermo Scientific | Exploris_Dabney | 2019-07-19 | 2019-07-19 |