This is a demonstration of how we use Prosit to predict peptide CID spectra of non-human origins. The original CSV files cannot be found (APEX2-NES, 8/17/22 and avidin, 8/19/22).
Two CSV files were created separately for FLAG-APEX2-NES and chicken avidin, based on the instruction below:
Tryptic peptides of interest were pasted to CSV files. For methionine-containing peptides, both intact and oxidized (ox) versions were included. Collision energy was set to 35. Charge states of interest were 2 and 3. Fragmentation method was set to CID for LTQ Orbitrap Velos Pro.
The intensity prediction model and iRT prediction model were selected as indicated above.
Output format was set to NIST .MSP.
See attached documents for CSV files submitted to Prosit (07/20/23) and rebuilt spectral libraries. APEX2 library: Task 6624D293ADD81E2DABA38EC490308E9; avidin library: Task BCAE3E6CD59FCD346627A53F5CBCF45C.
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