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Which peptides should be measured for a protein target?
Finding the Best Peptide
Synthesize protein
Digest with trypsin
Analyze by SRM
Finding the Best Peptide:
Apolipoprotein B100
Know Your Analytes
Signal Intensity
Chromatography
Fragmentation
Retention Time
Peptide Instability in the Autosampler
Peptide Stability |
Apolipoprotein B100
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