MS1 MS2 SWATH Study

2019-10-19

MS1 Peptide Ion Intensity Chromatograms in MS2 (SWATH) Data Independent Acquisitions. Improving Post Acquisition Analysis of Proteomic Experiments.

 

Authors: Matthew J. Rardin, Birgit Schilling, Lin-Yang Cheng, Brendan X. MacLean, Dylan J. Sorensen, Alexandria K. Sahu, Michael J. MacCoss, Olga Vitek, and Bradford W. Gibson.

 

Data sets acquired on a TripleTOF 5600 mass spectrometer and uploaded to Panorama:

 

  • MS1 and SWATH-MS – Kinase Inhibitor Study for quantifying changes in endogenous phosphopeptide isomers YHGHpS293MSDPGVSYR and YHGHSMSDPGVpS300YR from PDHE1a (3 injection replicates), go to Kinase Inhibitor Study in Panorama, download Kinase Inhibitor Study.
  • MS1 and SWATH-MS – dilution curve of heavy labeled phosphopeptide isomers YHGHpS293MSDPGVSYR and YHGHSMSDPGVpS300YR from PDHE1a (3 injection replicates), download various isomer ratio sub files: 16:1/1:168:1/1:84:1/1:4, 1:2, 1:1.

 

 

  • MS1 and SWATH-MS – MS1 and MS2 Data Processing Study for removal of interferences using mitochondrial lysates at a concentration of 100 ng or 33 ng (2 process replicates, 3 injection replicates), go to Data Processing Study in Panorama, download Data Processing Study (sample legend).