sp|P0A7D4|PURA_ECOLI: /JBEI/T.S. Batth et al. Metab. Eng. 2014 - A targeted proteomics toolkit for high-throughput absolute quantification of Escherichia coli proteins/Methods Development - 5500Q-trap data

sp|P0A7D4|PURA_ECOLI

Methods Development - 5500Q-trap data

Protein

File	Purine & Pyrmidine Biosynthesis.zip 1 MB SkyP file (link only, 1KB) Full Skyline file Copy relative URL to clipboard Copy full URL to clipboard
Description	Adenylosuccinate synthetase OS=Escherichia coli (strain K12) GN=purA PE=1 SV=2
Decoy	false
Note

Sequence Coverage

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By: Replicate: Modified forms: Combined Stacked

The number displayed on each peptide box is the rank
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M	G	N	N	V	V	V	L	G	T	Q	W	G	D	E	G	K	G	K	I	V	D	L	L	T	E	R	A	K	Y	V	V	R	Y	Q	G	G	H	N	A	G	H	T	L	V	I	N	G	E	K	T	V	L	H	L	I	P	S	G	I	L	R	E	N	V	T	S	I	I	G	N	G	V	V	L	S	P	A	A	L	M	K	E	M	K	E	L	E	D	R	G	I	P	V	R	E	R	L	L	L

101										111										121										131										141										151										161										171										181										191
S	E	A	C	P	L	I	L	D	Y	H	V	A	L	D	N	A	R	E	K	A	R	G	A	K	A	I	G	T	T	G	R	G	I	G	P	A	Y	E	D	K	V	A	R	R	G	L	R	V	G	D	L	F	D	K	E	T	F	A	E	K	L	K	E	V	M	E	Y	H	N	F	Q	L	V	N	Y	Y	K	A	E	A	V	D	Y	Q	K	V	L	D	D	T	M	A	V	A	D	I	L	T	S

201										211										221										231										241										251										261										271										281										291
M	V	V	D	V	S	D	L	L	D	Q	A	R	Q	R	G	D	F	V	M	F	E	G	A	Q	G	T	L	L	D	I	D	H	G	T	Y	P	Y	V	T	S	S	N	T	T	A	G	G	V	A	T	G	S	G	L	G	P	R	Y	V	D	Y	V	L	G	I	L	K	A	Y	S	T	R	V	G	A	G	P	F	P	T	E	L	F	D	E	T	G	E	F	L	C	K	Q	G	N	E	F	G	A

301										311										321										331										341										351										361										371										381										391
T	T	G	R	R	R	R	T	G	W	L	D	T	V	A	V	R	R	A	V	Q	L	N	S	L	S	G	F	C	L	T	K	L	D	V	L	D	G	L	K	E	V	K	L	C	V	A	Y	R	M	P	D	G	R	E	V	T	T	T	P	L	A	A	D	D	W	K	G	V	E	P	I	Y	E	T	M	P	G	W	S	E	S	T	F	G	V	K	D	R	S	G	L	P	Q	A	A	L	N	Y	I

401										411										421										431
K	R	I	E	E	L	T	G	V	P	I	D	I	I	S	T	G	P	D	R	T	E	T	M	I	L	R	D	P	F	D	A

Annotations for sp|P0A7D4|PURA_ECOLI

Description	Adenylosuccinate synthetase (short name: AMPSase)
Gene name	purA
Organisms	Escherichia coli Escherichia coli (strain 55989 / EAEC) Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) Escherichia coli (strain K12 / DH10B) Escherichia coli (strain K12 / MC4100 / BW2952) Escherichia coli (strain K12) Escherichia coli (strain SE11) Escherichia coli (strain SMS-3-5 / SECEC) Escherichia coli (strain UTI89 / UPEC) Escherichia coli O127:H6 (strain E2348/69 / EPEC) Escherichia coli O139:H28 (strain E24377A / ETEC) Escherichia coli O157:H7 Escherichia coli O157:H7 (strain EC4115 / EHEC) Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) Escherichia coli O1:K1 / APEC Escherichia coli O45:K1 (strain S88 / ExPEC) Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) Escherichia coli O6:K15:H31 (strain 536 / UPEC) Escherichia coli O7:K1 (strain IAI39 / ExPEC) Escherichia coli O8 (strain IAI1) Escherichia coli O81 (strain ED1a) Escherichia coli O9:H4 (strain HS)

Genbank IDs	GIs	Swiss-Prot Accessions	Swiss-Prot Names	Ensembl	IPI Numbers	GO Categories	Molecular Functions	Biological Processes
YP_002389643.1 YP_859846.1 YP_002405593.1 YP_002331952.1 NP_418598.1 YP_002400723.1 NP_290807.1 YP_001746572.1 YP_002929080.1 YP_002273719.1 YP_492319.1 YP_001726767.1 YP_001460959.1 YP_002415307.1 YP_672264.1 NP_313180.1 YP_001732943.1 YP_543712.1 YP_002295749.1 YP_002394249.1 YP_002410503.1 NP_757109.1 YP_001465676.1	none loaded	B1IT29 B7NTN3 B1LQJ7 B7MKY1 P0A7D6 P12283 P0A7D5 A1AJ82 B1XDS8 B7MSJ5 B5Z2I3 B7M8T7 B6I281 B7NGB1 P0A7D4 Q2M6C8 C4ZR54 A7ZV47 B7LC36 Q0T9L6 Q1R383 A8A7S2 B7UQI6	PURA_ECOLC PURA_ECO7I PURA_ECOSM PURA_ECO45 PURA_ECO57 PURA_ECOL6 PURA_ECOK1 PURA_ECODH PURA_ECO81 PURA_ECO5E PURA_ECO8A PURA_ECOSE PURA_ECOLU PURA_ECOLI PURA_ECOBW PURA_ECO24 PURA_ECO55 PURA_ECOL5 PURA_ECOUT PURA_ECOHS PURA_ECO27	none loaded	none loaded	GO:0000287 GO:0004019 GO:0005525 GO:0005737 GO:0044208 GO:0006164 GO:0006974 GO:0015949 GO:0016020 GO:0046086	Ligase	Purine biosynthesis

Peptides

Select All Select None Show Selected Show Unselected Show All	Peptide Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Peptide Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Missed Cleavages Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Avg. Measured RT Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Predicted RT Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	RT Score Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
	YVDYVLGILK	1181.6696	0	18.2243
	EVTTTPLAADDWK	1445.7039	0	11.4126

Chromatograms

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Summary Charts

Peak Areas

Retention Times