sp|P00561|AK1H_ECOLI: /JBEI/T.S. Batth et al. Metab. Eng. 2014 - A targeted proteomics toolkit for high-throughput absolute quantification of Escherichia coli proteins/Methods Development - 5500Q-trap data

sp|P00561|AK1H_ECOLI

Methods Development - 5500Q-trap data

Protein

File	Lysine, Threonine, Methionine Biosynthesis.zip 1 MB SkyP file (link only, 1KB) Full Skyline file Copy relative URL to clipboard Copy full URL to clipboard
Description	Bifunctional aspartokinase/homoserine dehydrogenase 1 OS=Escherichia coli (strain K12) GN=thrA PE=1 SV=2
Decoy	false
Note

Sequence Coverage

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By: Replicate: Modified forms: Combined Stacked

The number displayed on each peptide box is the rank
1										11										21										31										41										51										61										71										81										91
M	R	V	L	K	F	G	G	T	S	V	A	N	A	E	R	F	L	R	V	A	D	I	L	E	S	N	A	R	Q	G	Q	V	A	T	V	L	S	A	P	A	K	I	T	N	H	L	V	A	M	I	E	K	T	I	S	G	Q	D	A	L	P	N	I	S	D	A	E	R	I	F	A	E	L	L	T	G	L	A	A	A	Q	P	G	F	P	L	A	Q	L	K	T	F	V	D	Q	E	F	A	Q

101										111										121										131										141										151										161										171										181										191
I	K	H	V	L	H	G	I	S	L	L	G	Q	C	P	D	S	I	N	A	A	L	I	C	R	G	E	K	M	S	I	A	I	M	A	G	V	L	E	A	R	G	H	N	V	T	V	I	D	P	V	E	K	L	L	A	V	G	H	Y	L	E	S	T	V	D	I	A	E	S	T	R	R	I	A	A	S	R	I	P	A	D	H	M	V	L	M	A	G	F	T	A	G	N	E	K	G	E	L	V

201										211										221										231										241										251										261										271										281										291
V	L	G	R	N	G	S	D	Y	S	A	A	V	L	A	A	C	L	R	A	D	C	C	E	I	W	T	D	V	D	G	V	Y	T	C	D	P	R	Q	V	P	D	A	R	L	L	K	S	M	S	Y	Q	E	A	M	E	L	S	Y	F	G	A	K	V	L	H	P	R	T	I	T	P	I	A	Q	F	Q	I	P	C	L	I	K	N	T	G	N	P	Q	A	P	G	T	L	I	G	A	S	R	D

301										311										321										331										341										351										361										371										381										391
E	D	E	L	P	V	K	G	I	S	N	L	N	N	M	A	M	F	S	V	S	G	P	G	M	K	G	M	V	G	M	A	A	R	V	F	A	A	M	S	R	A	R	I	S	V	V	L	I	T	Q	S	S	S	E	Y	S	I	S	F	C	V	P	Q	S	D	C	V	R	A	E	R	A	M	Q	E	E	F	Y	L	E	L	K	E	G	L	L	E	P	L	A	V	T	E	R	L	A	I	I	S

401										411										421										431										441										451										461										471										481										491
V	V	G	D	G	M	R	T	L	R	G	I	S	A	K	F	F	A	A	L	A	R	A	N	I	N	I	V	A	I	A	Q	G	S	S	E	R	S	I	S	V	V	V	N	N	D	D	A	T	T	G	V	R	V	T	H	Q	M	L	F	N	T	D	Q	V	I	E	V	F	V	I	G	V	G	G	V	G	G	A	L	L	E	Q	L	K	R	Q	Q	S	W	L	K	N	K	H	I	D	L	R	V

501										511										521										531										541										551										561										571										581										591
C	G	V	A	N	S	K	A	L	L	T	N	V	H	G	L	N	L	E	N	W	Q	E	E	L	A	Q	A	K	E	P	F	N	L	G	R	L	I	R	L	V	K	E	Y	H	L	L	N	P	V	I	V	D	C	T	S	S	Q	A	V	A	D	Q	Y	A	D	F	L	R	E	G	F	H	V	V	T	P	N	K	K	A	N	T	S	S	M	D	Y	Y	H	Q	L	R	Y	A	A	E	K	S	R

601										611										621										631										641										651										661										671										681										691
R	K	F	L	Y	D	T	N	V	G	A	G	L	P	V	I	E	N	L	Q	N	L	L	N	A	G	D	E	L	M	K	F	S	G	I	L	S	G	S	L	S	Y	I	F	G	K	L	D	E	G	M	S	F	S	E	A	T	T	L	A	R	E	M	G	Y	T	E	P	D	P	R	D	D	L	S	G	M	D	V	A	R	K	L	L	I	L	A	R	E	T	G	R	E	L	E	L	A	D	I	E

701										711										721										731										741										751										761										771										781										791
I	E	P	V	L	P	A	E	F	N	A	E	G	D	V	A	A	F	M	A	N	L	S	Q	L	D	D	L	F	A	A	R	V	A	K	A	R	D	E	G	K	V	L	R	Y	V	G	N	I	D	E	D	G	V	C	R	V	K	I	A	E	V	D	G	N	D	P	L	F	K	V	K	N	G	E	N	A	L	A	F	Y	S	H	Y	Y	Q	P	L	P	L	V	L	R	G	Y	G	A	G	N	D

801										811
V	T	A	A	G	V	F	A	D	L	L	R	T	L	S	W	K	L	G	V

Annotations for sp|P00561|AK1H_ECOLI

Description	Bifunctional aspartokinase/homoserine dehydrogenase 1
Gene name	thrA
Organisms	Escherichia coli Escherichia coli (strain K12)

Genbank IDs	GIs	Swiss-Prot Accessions	Swiss-Prot Names	Ensembl	IPI Numbers	GO Categories	Biological Processes	Molecular Functions
YP_488308.1 NP_414543.1	none loaded	P00561 Q47659 Q6LEL0	AK1H_ECOLI	none loaded	none loaded	GO:0004072 GO:0004412 GO:0005524 GO:0009088 GO:0009089 GO:0009090 GO:0016597 GO:0050661	Amino-acid biosynthesis Threonine biosynthesis	Kinase Oxidoreductase Transferase

Peptides

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	GELVVLGR	841.5021	0	9.4749
	FFAALAR	794.4439	0	10.5760

Chromatograms

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Peak Areas

Retention Times