sp|P0A6W9|GSH1_ECOLI: /JBEI/T.S. Batth et al. Metab. Eng. 2014 - A targeted proteomics toolkit for high-throughput absolute quantification of Escherichia coli proteins/Methods Development - 5500Q-trap data

sp|P0A6W9|GSH1_ECOLI

Methods Development - 5500Q-trap data

Protein

File	Glutathione Biosynthesis.zip 198 KB SkyP file (link only, 1KB) Full Skyline file Copy relative URL to clipboard Copy full URL to clipboard
Description	Glutamate--cysteine ligase OS=Escherichia coli (strain K12) GN=gshA PE=1 SV=1
Decoy	false
Note

Sequence Coverage

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By: Replicate: Modified forms: Combined Stacked

The number displayed on each peptide box is the rank
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M	I	P	D	V	S	Q	A	L	A	W	L	E	K	H	P	Q	A	L	K	G	I	Q	R	G	L	E	R	E	T	L	R	V	N	A	D	G	T	L	A	T	T	G	H	P	E	A	L	G	S	A	L	T	H	K	W	I	T	T	D	F	A	E	A	L	L	E	F	I	T	P	V	D	G	D	I	E	H	M	L	T	F	M	R	D	L	H	R	Y	T	A	R	N	M	G	D	E	R	M	W

101										111										121										131										141										151										161										171										181										191
P	L	S	M	P	C	Y	I	A	E	G	Q	D	I	E	L	A	Q	Y	G	T	S	N	T	G	R	F	K	T	L	Y	R	E	G	L	K	N	R	Y	G	A	L	M	Q	T	I	S	G	V	H	Y	N	F	S	L	P	M	A	F	W	Q	A	K	C	G	D	I	S	G	A	D	A	K	E	K	I	S	A	G	Y	F	R	V	I	R	N	Y	Y	R	F	G	W	V	I	P	Y	L	F	G	A

201										211										221										231										241										251										261										271										281										291
S	P	A	I	C	S	S	F	L	Q	G	K	P	T	S	L	P	F	E	K	T	E	C	G	M	Y	Y	L	P	Y	A	T	S	L	R	L	S	D	L	G	Y	T	N	K	S	Q	S	N	L	G	I	T	F	N	D	L	Y	E	Y	V	A	G	L	K	Q	A	I	K	T	P	S	E	E	Y	A	K	I	G	I	E	K	D	G	K	R	L	Q	I	N	S	N	V	L	Q	I	E	N	E	L	Y

301										311										321										331										341										351										361										371										381										391
A	P	I	R	P	K	R	V	T	R	S	G	E	S	P	S	D	A	L	L	R	G	G	I	E	Y	I	E	V	R	S	L	D	I	N	P	F	S	P	I	G	V	D	E	Q	Q	V	R	F	L	D	L	F	M	V	W	C	A	L	A	D	A	P	E	M	S	S	S	E	L	A	C	T	R	V	N	W	N	R	V	I	L	E	G	R	K	P	G	L	T	L	G	I	G	C	E	T	A	Q	F

401										411										421										431										441										451										461										471										481										491
P	L	P	Q	V	G	K	D	L	F	R	D	L	K	R	V	A	Q	T	L	D	S	I	N	G	G	E	A	Y	Q	K	V	C	D	E	L	V	A	C	F	D	N	P	D	L	T	F	S	A	R	I	L	R	S	M	I	D	T	G	I	G	G	T	G	K	A	F	A	E	A	Y	R	N	L	L	R	E	E	P	L	E	I	L	R	E	E	D	F	V	A	E	R	E	A	S	E	R	R	Q	Q

501										511
E	M	E	A	A	D	T	E	P	F	A	V	W	L	E	K	H	A

Annotations for sp|P0A6W9|GSH1_ECOLI

Description	Glutamate--cysteine ligase
Gene name	gshA
Organisms	Escherichia coli Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) Escherichia coli (strain K12 / DH10B) Escherichia coli (strain K12 / MC4100 / BW2952) Escherichia coli (strain K12) Escherichia coli (strain SE11) Escherichia coli (strain UTI89 / UPEC) Escherichia coli O45:K1 (strain S88 / ExPEC) Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) Escherichia coli O6:K15:H31 (strain 536 / UPEC) Escherichia coli O81 (strain ED1a) Escherichia coli O9:H4 (strain HS)

Genbank IDs	GIs	Swiss-Prot Accessions	Swiss-Prot Names	Ensembl	IPI Numbers	GO Categories	Biological Processes	Molecular Functions
YP_002392594.1 YP_001731590.1 YP_001724015.1 YP_002399027.1 NP_755127.1 YP_002927626.1 YP_670543.1 NP_417173.1 YP_001459457.1 YP_002294216.1 YP_490902.1 YP_542037.1	none loaded	Q0TEI7 B7MKG2 B1XCM1 P06980 P0A6X0 P78228 A8A3H0 B1IUY6 P0A6W9 C4ZYT8 Q1R808 B6I679 B7MYJ3	GSH1_ECOL5 GSH1_ECO45 GSH1_ECODH GSH1_ECOL6 GSH1_ECOHS GSH1_ECOLC GSH1_ECOLI GSH1_ECOBW GSH1_ECOUT GSH1_ECOSE GSH1_ECO81	none loaded	none loaded	GO:0004357 GO:0005524 GO:0006750 GO:0006972 GO:0046872 GO:0071243 GO:0071288	Glutathione biosynthesis	Ligase

Peptides

Select All Select None Show Selected Show Unselected Show All	Peptide Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Peptide Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Missed Cleavages Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Avg. Measured RT Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Predicted RT Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	RT Score Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
	SGESPSDALLR	1130.5568	0	5.5533
	GGIEYIEVR	1034.5397	0	6.4617

Chromatograms

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Peak Areas

Retention Times