Measurement of Nrf2-Regulated Antioxidant Response Proteins as Potential Biomarkers for Radiation Injury Assessment
Liu K, Singer E, Cohn W, Micewicz ED, McBride WH, Whitelegge JP, Loo JA. Time-Dependent Measurement of Nrf2-Regulated Antioxidant Response to Ionizing Radiation towards Identifying Potential Protein Biomarkers for Acute Radiation Injury. PROTEOMICS – Clin Appl [Internet]. 0(ja):1900035. Available from: https://onlinelibrary.wiley.com/doi/abs/10.1002/prca.201900035
- Organism: Mus musculus
- Instrument: Q Exactive
radiation, biomarker, parallel reaction monitoring
Using targeted mass spectrometry, we examined the response of Nrf2-ARE-dependent enzymes in mouse bone marrow collected at various time points (8 hours to 4 days) after 6 Gy total body irradiation in two mouse strains and both genders. Relative protein abundances and statistical significance compared to unirradiated control mice were analyzed in Skyline and MSstats.
Created on 1/15/19, 10:21 PM