Ehime HBFP - Tomato TGC

Ehime HBFP - Tomato TGC
Clustergrammer Heatmap
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E4HN 69 Wk F_M_120k.sky.zip2020-08-11 12:22:4620283984
E4HN 69 Wk F_M_240k_for Cit+9.sky.zip2020-08-11 12:22:39106684
Pilot Experiment APOE3 3 Mice (21 DBS_3Plasma_2Brain).sky.zip2020-08-11 12:22:3620273829
Pilot Experiment APOE3 3 Mice (21 DBS_3Plasma_2Brain)_240k.sky.zip2020-08-11 12:22:33106625
Pilot Kinetics Experiment CVN Mice 120k.sky.zip2020-08-11 12:22:322018248
5572_Plate1LC2_07232020.sky.zip2020-08-11 12:19:3890505099
5572_Plate2LC1_07222020.sky.zip2020-08-11 12:19:2310122123113
5572_Plate2LC2_07232020.sky.zip2020-08-11 12:18:4990505099
5572_Plate3LC1_07282020.sky.zip2020-08-11 12:18:3210122123113
5572_Plate3LC2_07282020.sky.zip2020-08-11 12:17:5590505099
5572_Plate4LC1_07302020.sky.zip2020-08-11 12:17:3510122123113
5572_Plate4LC2_07312020.sky.zip2020-08-11 12:17:0090505099
5572_Plate1LC1_07222020.sky.zip2020-08-11 12:16:4510122123113
vanGelder_Kinase_phosphorylation_2020-07-30_16-35-41.sky.zip2020-08-06 12:03:144510502
Common_Wheat_2020-07-30_13-16-10.sky.zip2020-08-03 08:35:4013224418648
Representative_Response_2020-07-30_13-25-50.sky.zip2020-08-03 08:35:401322432137
Durum_Wheat_2020-07-30_13-31-28.sky.zip2020-08-03 08:35:4013224419648
Einkorn_2020-07-30_13-53-08.sky.zip2020-08-03 08:35:4013224419054
Emmer_2020-07-30_13-58-02.sky.zip2020-08-03 08:35:4013224418851
Spelt_2020-07-30_14-09-32.sky.zip2020-08-03 08:35:4013224418248
Identification_Peptides_2020-07-21_14-05-35.sky.zip2020-08-03 08:32:1713486483916
Identification_Peptides_Selection_2020-07-30_14-12-39.sky.zip2020-08-03 08:32:171234351754
BSA-QC_68fmol_Jul2020_Impact-II_minPanorama_2020-07-30_12-55-38.sky.zip2020-07-30 12:57:29131402167
NIST PTM.sky.zip2020-07-29 09:37:022501374113
LINCS_P100_DIA_Plate97_annotated_minimized_2020-06-29_18-13-03.sky.zip2020-06-29 15:20:4490961922,76490
BSA-QC_68fmol_Jun2020_Impact-II_minPanorama_2020-06-28_22-05-23.sky.zip2020-06-28 22:10:23131402165
19_8_27_LARP1_Kinase_Reaction_2020-06-18_10-51-35.sky.zip2020-06-24 21:25:59264762373
Phospho_CDK2_Silencing_Experiment_Human_2020-06-23_16-21-00.sky.zip2020-06-24 21:25:5911921636
20_6_Phospho_SAMe_Combined.sky.zip2020-06-24 21:25:582,77924,31330,00390,00933
Sky0 ACE2 TMPRSS2 cell lines_2020-06-18_16-55-27.sky.zip2020-06-18 13:38:456252919732
LINCS_P100_DIA_Plate96_annotated_minimized_2020-06-11_19-02-30.sky.zip2020-06-11 16:24:3290961922,76493
AMBPb_2020-06-01_14-39-27.sky.zip2020-06-11 15:34:1311261
AMBP_2020-06-01_17-29-42.sky.zip2020-06-11 15:34:13112668
AMBPc_2020-06-02_12-38-26.sky.zip2020-06-11 15:34:1311262
B2Mb_2020-06-02_15-19-17.sky.zip2020-06-11 15:34:1311268
B2M_2020-06-02_17-56-29.sky.zip2020-06-11 15:34:13112663
HBB_2020-06-03_11-25-07.sky.zip2020-06-11 15:34:13112672
IGHA1_2020-06-03_14-07-57.sky.zip2020-06-11 15:34:131121069
IGHA1b_2020-06-03_16-47-13.sky.zip2020-06-11 15:34:13112101
IGHA1c_2020-06-04_12-45-14.sky.zip2020-06-11 15:34:13112102
LYZ_2020-06-04_15-26-23.sky.zip2020-06-11 15:34:13112460
LYZb_2020-06-04_18-12-30.sky.zip2020-06-11 15:34:13112412
SERPINF1_2020-06-05_12-55-50.sky.zip2020-06-11 15:34:13112669
SERPINF1b_2020-06-05_16-03-56.sky.zip2020-06-11 15:34:1311262
Sky1 Synthetic SARS-CoV2 proteome library_2020-05-30_00-33-22.sky.zip2020-06-09 13:21:56121431351,2121
Sky3 52 patients nano PRM-MS2 raw_2020-05-30_00-38-21.sky.zip2020-06-09 13:20:236345734055
Sky4 5 positive patients nano PRM-MS2 refined_2020-05-30_00-52-02.sky.zip2020-06-09 13:20:22634492945
Sky6 11 positive patients micro PRM-MS2 refined_2020-05-30_00-19-04.sky.zip2020-06-09 13:11:206345527311
Sky5 91 patients micro PRM-MS2 raw_2020-05-30_00-57-41.sky.zip2020-06-09 13:11:206345527391
Sky2 Dilution series SARS Cov2 nano and microflow_2020-05-30_00-30-29.sky.zip2020-06-09 13:04:536345737118
20191031_Cdc55AID_PRM_l1_manual3_2020-05-02_10-59-22.sky.zip2020-06-02 20:16:492828562164
20191031_Cdc55AID_PRM_l2_manual3_2020-05-13_10-32-35.sky.zip2020-06-02 20:16:492627542064
20191031_Cdc55AID_PRM_l3_manual3_2020-05-13_11-25-38.sky.zip2020-06-02 20:16:492424481824
ARMeD_NEDP1_2019-11-02_13-41-15.sky.zip2020-05-25 20:17:53751481622,58918
Batch1412_2020-05-12_15-21-49.sky.zip2020-05-19 20:17:272141254
Batch1553_2020-05-12_15-28-16.sky.zip2020-05-19 20:17:272141263
Batch1508_2020-05-12_15-31-44.sky.zip2020-05-19 20:17:272151639
Batch1015_2020-05-12_15-39-40.sky.zip2020-05-19 20:17:272151631
Batch1134_2020-05-12_15-41-42.sky.zip2020-05-19 20:17:272141235
Batch1139_2020-05-12_15-45-12.sky.zip2020-05-19 20:17:272141238
Batch1039_2020-05-12_15-46-04.sky.zip2020-05-19 20:17:272141265
Batch1039b_2020-05-12_15-47-09.sky.zip2020-05-19 20:17:272141211
Batch1135_2020-05-12_15-49-04.sky.zip2020-05-19 20:17:262141264
Batch1553rep_2020-05-12_15-50-08.sky.zip2020-05-19 20:17:262141213
Batch1059_2020-05-12_15-51-24.sky.zip2020-05-19 20:17:262041284
Batch 1143_2020-05-12_15-53-25.sky.zip2020-05-19 20:17:262141265
Precision_2020-05-18_19-01-24.sky.zip2020-05-19 20:17:262141219
PRM_60min_2020-05-12_18-57-21.sky.zip2020-05-19 20:16:45417201228
PRM_9min_2020-05-18_21-07-56.sky.zip2020-05-19 20:16:44299668
PRM_9min_2020-05-19_07-53-44.sky.zip2020-05-19 20:16:10299668
LINCS_P100_DIA_Plate29_annotated_minimized_2020-05-18_18-50-11.sky.zip2020-05-18 15:59:1690961921,02896
DIAassayLibrary_Gasac-Gill_Rev2_2020-05-15_18-31-25.sky.zip2020-05-15 19:31:421,6914,7464,87926,1230
DIAassayLibrary_Gasac-Gill_Rev1_2020-05-15_17-01-05.sky.zip2020-05-15 17:38:291,5075,0745,10425,3220
Inflammatory_peptides_2020-05-13_13-35-09.sky.zip2020-05-13 10:58:1317494921860
Manually_filtered_peptides_2020-05-13_08-27-56.sky.zip2020-05-13 05:51:586253,2053,20513,12660
Prefiltering_CSF_document_2020-05-12_09-28-28.sky.zip2020-05-12 07:21:051,2225,9545,95422,91166
ResponseCurve-EGFR-LTQLGTFEDHFLSLQR_2020-01-28_09-46-19.sky.zip2020-05-11 16:35:09112434
ResponseCurve-CCND1-AYPDANLLNDR_2020-01-28_09-46-04.sky.zip2020-05-11 16:35:09112644
ResponseCurve-FOLH1-TYSVSFDSLFSAVK_2020-01-28_09-46-45.sky.zip2020-05-11 16:35:09112638
ResponseCurve-MUC1-DISEMFLQIYK_2020-01-28_09-47-16.sky.zip2020-05-11 16:35:09112428
ResponseCurve-PSA-LSEPAETDAVK_2020-01-28_09-47-42.sky.zip2020-05-11 16:35:09112644
ResponseCurve-SPINK1-QTSILIQK_2020-01-28_09-48-12.sky.zip2020-05-11 16:35:09112632
ResponseCurve-MMP9-LGLGADVAQVTGALR_2020-01-28_09-47-01.sky.zip2020-05-11 16:35:09112643
ResponseCurve-NCOA2-LLQDSSSPVDLAK_2020-01-28_09-47-28.sky.zip2020-05-11 16:35:09112442
ResponseCurve-SPRC-LEAGDHPVELLAR_2020-01-28_09-48-25.sky.zip2020-05-11 16:35:09112442
ResponseCurve-TGFB1-GGEIEGFR_2020-01-28_09-48-56.sky.zip2020-05-11 16:35:09112436
ResponseCurve-VEGFA-FMDVYQR_2020-01-28_09-49-13.sky.zip2020-05-11 16:35:09112433
ResponseCurve-CAMKK2-SLSAPGNLLTK_2020-01-28_09-45-49.sky.zip2020-05-11 16:35:09112442
ResponseCurve-ERG_pan-VIVPADPTLWSTDHVR_2020-01-28_09-46-32.sky.zip2020-05-11 16:35:09112643
ResponseCurve-SMAD4-YCQYAFDLK_2020-01-28_09-47-57.sky.zip2020-05-11 16:35:09112444
ResponseCurve-TFF3-VDCGYPHVTPK_2020-01-28_09-48-41.sky.zip2020-05-11 16:35:09112444
ResponseCurve-ANXA2-GLGTDEDSLIEIICSR_2020-01-28_09-49-31.sky.zip2020-05-11 16:35:09112441
CPDR-FFPE-ID-1193_2020-01-29_12-44-32.sky.zip2020-05-11 16:23:121616328224
CPDR-FFPE-ID-859_2020-01-29_12-41-32.sky.zip2020-05-11 16:23:121616328423
CPDR-FFPE-ID-1250_2020-01-29_12-41-18.sky.zip2020-05-11 16:23:121616328229
CPDR-FFPE-ID-1376_2020-01-29_14-56-40.sky.zip2020-05-11 16:23:121616328027
CPDR-FFPE-ID-1689_2020-01-29_12-40-48.sky.zip2020-05-11 16:23:121616328425
CPDR-FFPE-ID-636_2020-01-28_13-43-56.sky.zip2020-05-11 16:23:121616328632
CPDR-FFPE-ID-779_2020-01-28_13-41-34.sky.zip2020-05-11 16:23:121616328225
CPDR-FFPE-ID-850_2020-01-29_12-24-07.sky.zip2020-05-11 16:23:121616328223
Quantitative assay of targeted proteome in trichome glandular cells using large-scale selected reaction monitoring strategy

  • Organism: Solanum lycopersicum
  • Instrument: QTRAP 5500
  • SpikeIn: No
  • Keywords: Tomato type VI trichome glandular cell
  • Lab head: Nobuaki Takemori
Abstract
Background: Glandular trichomes found in vascular plants are called natural cell factories because they synthesize and store secondary metabolites in the glandular cell. In order to systematically understand the metabolic processes in the glandular cells, it is indispensable to analyze the cellular proteome dynamics. The conventional proteomics methods based on mass spectrometry have been enabled large-scale protein analysis, but requires a large number of trichome samples for in-depth analysis and is not suitable for rapid and sensitive quantification of targeted proteins. Results: Here we present a high-throughput strategy for quantifying targeted proteins in a specific trichome glandular cell, using selected reaction monitoring (SRM) assay. SRM assay platform, targeting the proteins in tomato type VI trichome gland cells as a model study, demonstrated its effectiveness in multiple protein quantitation from a limited sample amount. A large-scale SRM assay developed using triple quadrupole mass spectrometer online connected to nanoflow liquid chromatography has enough capability to accurately measure the expression level of 221 targeted proteins contained in the glandular cell sample recovered from the 100 glandular trichomes within 120 minutes. Conclusions: Targeted proteomics approach using established SRM assays makes it possible to quantify the proteins of interest with minimum effort in the sampling. The remarkable success of the SRM assay developments by a simple experimental workflow will increase the plant biologist's accessibility to proteomics research for glandular trichome.
Experiment Description
The development of SRM assays was performedwith the Skyline software. The database search files from the ProteinPilot software (SCIEX) were used to build the MS spectral library containing only tryptic digested peptides (allowed one missed cleavage) with their confidence value > 0.9. The analysis of obtained SRM data was performed with Skyline. Endogenous peptide peaks in the acquired SRM chromatogram were selected manually based on chromatographic elution pattern and the dot product (dotp) value.
Sample Description
Tomato cultivar ‘Micro-Tom’ (S. lycopersicum L. cv. Microtom) was used for all the studies.
Created on 1/7/19, 9:05 PM