Cell cycle validation
Federspiel JD, Tandon P, Wilczewski CM, Wasson L, Herring LE, Venkatesh SS, Cristea IM, Conlon FL. Conservation and divergence of protein pathways in the vertebrate heart. PLoS biology. 2019 Sep 6;17(9):e3000437.
- Organism: Mus musculus, Sus scrofa, Xenopus laevis, Xenopus tropicalis
- Instrument: Q Exactive HF
- SpikeIn:
No
Cell cycle proteins found to be enriched in X. laevis in the discovery set were targeted for validation via PRM in a new set of replicates.
Heart samples were extracted in SDS buffer, precipitated, and processed by in-solution digestion prior to PRM analysis.
Created on 6/13/19, 9:56 AM