JBEI - Limonene Proteomic Data

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Alonso-Gutierrez-Limonene-manuscript-Metab Eng 2013_2014-03-06_09-12-25.sky.zip (202 KB)2018-06-11122424484
Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production

  • Organism: Escherichia coli
  • Instrument: AB Sciex 5500
  • SpikeIn: No
Abstract
Limonene is a valuable monoterpene used in the production of several commodity chemicals and medicinal compounds. Among them, perillyl alcohol (POH) is a promising anti-cancer agent that can be produced by hydroxylation of limonene. We engineered E. coli with a heterologous mevalonate pathway and limonene synthase for production of limonene followed by coupling with a cytochrome P450, which specifically hydroxylates limonene to produce POH. A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400 mg/L from glucose, substantially higher than has been achieved in the past. Incorporation of a cytochrome P450 to hydroxylate limonene yielded approximately 100 mg/L of POH. Further metabolic engineering of the pathway and in situ product recovery using anion exchange resins would make this engineered E. coli a potential production platform for any valuable limonene derivative.
Created on 6/11/18, 3:16 PM