Cristea Lab - Xenopus_PRM

Mechanical force induces rapid phosphorylation dependent signaling in Xenopus embryos

  • Organism: Xenopus laevis
  • Instrument: Q Exactive HF
  • SpikeIn: No
  • Keywords: Phosphorylation, Signaling
Abstract
Mechanical forces are involved in morphogenesis and differentiation in development. We investigated phosphorylation-dependent signaling in response to mechanical force stimulation in Xenopus laevis embryos.
Experiment Description
Validation of protein and phosphopeptide abundances.
Sample Description
(1) The amounts of F11r.L(pS275) upon different timepoints were analyzed in "F11R_phosphorylation_timeseries". (2) The amounts of Pak2.L(pS136), Pak2.L(pS193), and Map4.L(pS1001) upon different timepoints were analyzed in "Pak2_Map4_phosphorylation_timeseries". (3) The amounts of Pak2.L(pS193) with or without FAK inhibitor were analyzed in "Pak2_phosphorylation_FAKi". (4) The amounts of F11r.L(pS275) and Prkd1.L(pS696) with or without FAK inhibitor were analyzed in "Pkc_substrates_phosphorylation_FAKi". (5) A subset of abundances of EMT and MET proteins were analyzed in "EMT_MET_proteins_timeseries01". (6) A subset of abundances of EMT and MET proteins were analyzed in "EMT_MET_proteins_timeseries02".
Created on 8/6/18 8:52 AM
Clustergrammer Heatmap
Flag FileDownloadCreatedProteinsPeptidesPrecursorsTransitionsReplicates
Pkc_substrates_phosphorylation_FAKi_2018-08-03_11-58-31.sky.zip (54 MB)2018-08-06222106
Pak2_phosphorylation_FAKi_2018-08-03_12-26-43.sky.zip (77 MB)2018-08-0611146
EMT_MET_proteins_timeseries01_2018-08-03_11-55-42.sky.zip (16 MB)2018-08-0615363619712
F11R_phosphorylation_timeseries_2018-08-03_12-36-04.sky.zip (11 MB)2018-08-06111412
Pak2_Map4_phosphorylation_timeseries_2018-08-03_12-32-51.sky.zip (34 MB)2018-08-062331512
EMT_MET_proteins_timeseries02_2018-08-03_11-51-05.sky.zip (16 MB)2018-08-06714146112