Mechanical force induces rapid phosphorylation dependent signaling in Xenopus embryos
- Organism: Xenopus laevis
- Instrument: Q Exactive HF
Mechanical forces are involved in morphogenesis and differentiation in development. We investigated phosphorylation-dependent signaling in response to mechanical force stimulation in Xenopus laevis embryos.
Validation of protein and phosphopeptide abundances.
(1) The amounts of F11r.L(pS275) upon different timepoints were analyzed in "F11R_phosphorylation_timeseries". (2) The amounts of Pak2.L(pS136), Pak2.L(pS193), and Map4.L(pS1001) upon different timepoints were analyzed in "Pak2_Map4_phosphorylation_timeseries". (3) The amounts of Pak2.L(pS193) with or without FAK inhibitor were analyzed in "Pak2_phosphorylation_FAKi". (4) The amounts of F11r.L(pS275) and Prkd1.L(pS696) with or without FAK inhibitor were analyzed in "Pkc_substrates_phosphorylation_FAKi". (5) A subset of abundances of EMT and MET proteins were analyzed in "EMT_MET_proteins_timeseries01". (6) A subset of abundances of EMT and MET proteins were analyzed in "EMT_MET_proteins_timeseries02".
Created on 8/6/18 8:52 AM