UTurkuPlantBio - PSB33

UTurkuPlantBio - PSB33
Clustergrammer Heatmap
Flag FileDownloadCreatedProteinsPeptidesPrecursorsTransitionsReplicates
LINCS_P100_DIA_Plate80_annotated_minimized_2019-06-13_12-13-28.sky.zip (121 MB)2019-06-1390961922,76496
LINCS_GCP_Plate71b_annotated_minimized_2019-06-10_18-12-11.sky.zip (12 MB)2019-06-10818116283018
LINCS_GCP_Plate71a_annotated_minimized_2019-06-10_18-10-31.sky.zip (5 MB)2019-06-1081811628548
LINCS_P100_DIA_Plate77_annotated_minimized_2019-06-10_16-17-36.sky.zip (81 MB)2019-06-1090961922,76266
LINCS_P100_DIA_Plate70_annotated_minimized_2019-06-03_13-47-33.sky.zip (140 MB)2019-06-0890961922,76496
LINCS_P100_DIA_Plate82_annotated_minimized_2019-06-03_16-03-24.sky.zip (110 MB)2019-06-0890961922,76491
LINCS_P100_DIA_Plate76_annotated_minimized_2019-06-07_15-29-16.sky.zip (100 MB)2019-06-0790961922,75866
LINCS_P100_QC_Plate80_annotated_minimized_2019-06-04_16-27-57.sky.zip (7 MB)2019-06-04882417496
LINCS_P100_QC_Plate82_annotated_minimized_2019-06-04_15-46-59.sky.zip (8 MB)2019-06-04882418396
LINCS_P100_QC_Plate78_annotated_minimized_2019-06-03_15-57-25.sky.zip (7 MB)2019-06-03882316196
LINCS_P100_DIA_Plate70_annotated_minimized_2019-06-03_13-47-33.sky.zip (140 MB)2019-06-0390961922,76496
LINCS_P100_QC_Plate70_annotated_minimized_2019-06-03_13-48-35.sky.zip (7 MB)2019-06-03882416596
all combined_2019-03-21_20-40-14.sky.zip (46 MB)2019-05-31133575268,5337
20180518 multiplex quantification - APOA4 only samples_2018-05-18_12-13-20.sky.zip (265 MB)2019-05-311364864
20180518 multiplex quantification only samples_2018-05-18_11-58-07.sky.zip (380 MB)2019-05-3128357050268
20180509_prest-kinetics_2018-05-09_18-52-06.sky.zip (120 MB)2019-05-311613628684820
500 µE (PG-500uE_2ul_2019-05-16_15-23-09).sky.zip (7 MB)2019-05-292710510555811
Huidan-LHCSR3-Trypsin-PRM_FINAL2_2019-05-16_17-33-35.sky.zip (3 MB)2019-05-2920611225758
Huidan-LHCSR3-LysC-selected-tSIM-PRM_FINAL_2019-05-16_17-31-57.sky.zip (13 MB)2019-05-2931681366528
200µE (R5-8_V2_2019-05-16_15-31-29).sky.zip (8 MB)2019-05-2928979751616
matchedmatrixcalcurve_yeasthela_2019-05-28_13-23-23.sky.zip (1 GB)2019-05-283,22827,28927,28985,17542
matchedmatrixcalcurve_yeastwater_2019-05-24_12-06-14.sky.zip (1 GB)2019-05-243,73136,39336,39395,63942
20150514_Plasma_remove_points_2019-05-22_18-51-25.sky.zip (162 MB)2019-05-2220989862412
matchedmatrixcalcurve_csf_2019-05-22_14-34-12.sky.zip (531 MB)2019-05-224,00716,22916,22947,85630
matchedmatrixcalcurve_yeastyeast_2019-05-22_12-49-50.sky.zip (1 GB)2019-05-223,48230,60230,60290,48842
Janschitz et al_PRM_results_Panorama upload_2019-05-06_18-03-18.sky.zip (611 MB)2019-05-094475833712
LINCS_GCP_Plate76_annotated_minimized_2019-04-29_15-46-06.sky.zip (16 MB)2019-04-29818116287833
LINCS_P100_DIA_Plate51_annotated_minimized_2017-08-02_16-21-22.sky.zip (43 MB)2019-04-26909619292496
LINCS_P100_DIA_Plate41_annotated_minimized_2017-02-16_14-31-38.sky.zip (128 MB)2019-04-2690961921,00296
LINCS_P100_DIA_PlateAD2_annotated_minimized_2019-04-18_11-17-21.sky.zip (52 MB)2019-04-18909619278892
LINCS_P100_QC_PlateAD2_annotated_minimized_2019-04-18_11-15-52.sky.zip (6 MB)2019-04-18882413292
LINCS_GCP_Plate77_annotated_minimized_2019-04-17_09-22-00.sky.zip (19 MB)2019-04-17818116291233
LINCS_GCP_Plate75_annotated_minimized_2019-04-16_13-40-09.sky.zip (26 MB)2019-04-16818116291233
LINCS_GCP_Plate74_annotated_minimized_2019-04-16_13-21-11.sky.zip (24 MB)2019-04-16808016080533
LINCS_GCP_Plate73_annotated_minimized_2019-04-16_11-45-08.sky.zip (24 MB)2019-04-16818116289433
LINCS_P100_QC_Plate75_annotated_minimized_2019-04-08_16-33-18.sky.zip (4 MB)2019-04-08882415666
LINCS_P100_DIA_Plate75_annotated_minimized_2019-04-08_16-26-05.sky.zip (72 MB)2019-04-0890961922,76466
LINCS_P100_QC_Plate76_annotated_minimized_2019-04-08_15-34-30.sky.zip (5 MB)2019-04-08882415066
Dee Dee Luu et. al_The immunogenic microbial peptide RaxX represents an unclassified group of sulfated ribosomally synthesized and post-translationally modified peptides_2019-04-02_12-44-12.sky.zip (1 MB)2019-04-062221318
LINCS_P100_DIA_Plate73_annotated_minimized_2019-04-02_12-31-00.sky.zip (75 MB)2019-04-0290961922,76466
LINCS_P100_QC_Plate74a_annotated_minimized_2019-04-01_16-25-26.sky.zip (2 MB)2019-04-01882417436
LINCS_P100_DIA_Plate74a_annotated_minimized_2019-04-01_16-19-30.sky.zip (39 MB)2019-04-0190961922,76236
LINCS_P100_QC_Plate73_annotated_minimized_2019-03-28_10-49-18.sky.zip (5 MB)2019-03-28882416266
LINCS_P100_QC_Plate74_annotated_minimized_2019-03-27_16-36-40.sky.zip (6 MB)2019-03-27882417182
LINCS_P100_DIA_Plate74_annotated_minimized_2019-03-27_16-18-55.sky.zip (98 MB)2019-03-2790961922,76284
LINCS_P100_QC_Plate70_annotated_minimized_2019-03-13_14-12-46.sky.zip (7 MB)2019-03-13882416596
HLF with ladder_GUnames_2018-08-15_19-59-17.sky.zip (176 KB)2019-03-086045451
U87MG with ladder_GUnames_2018-08-15_20-02-38.sky.zip (429 KB)2019-03-08901181181
IgA with ladder_GUnames_2018-08-15_20-00-17.sky.zip (358 KB)2019-03-08801251251
HNE with ladder_GUnames_2018-08-15_19-59-46.sky.zip (174 KB)2019-03-086038381
PGM OG with ladder_GUnames_2018-08-15_20-01-34.sky.zip (370 KB)2019-03-08201061061
IgG with ladder_GUnames_2018-08-15_20-01-05.sky.zip (228 KB)2019-03-087061611
Fet NG with ladder_GUnames_2018-08-15_19-58-42.sky.zip (229 KB)2019-03-087063631
CBHI OG with ladder_GUnames_2018-08-15_19-58-20.sky.zip (93 KB)2019-03-082020201
CBHI NG with ladder_GUnames_2018-08-15_19-57-24.sky.zip (109 KB)2019-03-083028281
BLF with ladder_GUnames_2018-08-15_19-55-59.sky.zip (302 KB)2019-03-088085851
Fig3_U87MG_iRT_ladder_2018-08-15_19-50-07.sky.zip (429 KB)2019-03-08901191191
Fig5_SupFig3_Dextran_Spectral_Library_2018-08-15_19-53-02.sky.zip (40 KB)2019-03-08103181
Fig1_SupFig1_Ladder_tech_reps_2018-08-15_19-48-46.sky.zip (797 KB)2019-03-0810151510
Fig6_2019-01-26_23-48-44.sky.zip (63 MB)2019-03-088011634212
Fig2_SupFig2_iRT_linear.sky.zip (6 MB)2019-03-0820183216
PRM-Identifying the N-terminal amino acid for a TAG start codon_2019-03-04.sky.zip (3 MB)2019-03-05266583
PRM-Identifying the N-terminal amino acid for an ATG start codon_2019-03-04.sky.zip (1 MB)2019-03-05266563
Cortex_50_targets_PRM_finaL_20181212_2019-01-15_15-55-34.sky.zip (5 MB)2019-03-01551332582,2633
Pancreas_50_targets_PRM_final_20181210_2019-01-15_15-52-49.sky.zip (5 MB)2019-03-01631342602,0613
sasp_panel_ras_2019-02-26_13-24-23.sky.zip (51 MB)2019-02-2674730,02544,411576,8660
sasp_panel_irradiation_2019-02-26_13-00-53.sky.zip (17 MB)2019-02-261729,62414,598189,6470
sasp_panel_atazanavir_2019-02-26_11-45-06.sky.zip (27 MB)2019-02-2637615,60123,172301,0320
sasp_panel_all_inducers_2019-02-26_11-18-00.sky.zip (17 MB)2019-02-261729,62414,598189,6470
QEPlus_2019-02-05_05-55-27.sky.zip (23 MB)2019-02-0419192414469
QEPlus_2019-02-03_11-26-48.sky.zip (23 MB)2019-02-0319192414469
Gigas-6-10-DIA.sky.zip (1 GB)2019-01-309,04732,31233,292348,20120
2017-09-06-GigasDilutionCurve.sky.zip (118 MB)2019-01-301851511728
2017-09-06-Gigas-SRM-ReplicatesOnly-PostDilutionCurve-emma.sky.zip (244 MB)2019-01-3017474713795
WB_PK_bi_scFv_2019-01-18_23-49-08.sky.zip (2 MB)2019-01-2215107062
Plasma_PK_bi_scFv_2019-01-18_23-51-46.sky.zip (2 MB)2019-01-2215107058
Plasma_PK_bi_scFv_2019-01-18_23-51-46.sky.zip (2 MB)2019-01-1815107058
WB_PK_bi_scFv_2019-01-18_23-49-08.sky.zip (2 MB)2019-01-1815107062
PlasmaExp2B4-5_5x_Chromatogram_2019-01-17_08-10-03.sky.zip (815 KB)2019-01-1897971949311
PlasmaExp2B4-5_5x_Chromatogram_2_20180117_2019-01-17_12-35-37.sky.zip (815 KB)2019-01-1897971949311
MousePlasma_Exp2B7_Chromatogram_5x_2019-01-16_16-21-58.sky.zip (758 KB)2019-01-162121422021
20160309_MouseV2B12M6&7_refined_2019-01-16_16-20-54.sky.zip (15 MB)2019-01-16465110239039
20160914_Batch6results_refined_2019-01-16_16-20-19.sky.zip (13 MB)2019-01-16535410851045
20170215_Exp2B6.5_Chromatogram_5x_2019-01-16_16-19-40.sky.zip (294 KB)2019-01-161717341661
20170215_Exp2B6.5_Chromatogram_2.5x_2019-01-16_16-17-36.sky.zip (334 KB)2019-01-1649501004831
PlasmaExp2B4-5_5x_refined_2019-01-16_16-15-55.sky.zip (32 MB)2019-01-16979719496345
MousePlasmaExp2B4-5_Refined_2019-01-16_16-13-46.sky.zip (62 MB)2019-01-161661703401,69045
20160620_Plasma_Exp1_B4_M1_refined_2019-01-16_16-13-15.sky.zip (17 MB)2019-01-16676713462845
MousePlasmaExp2B4-5_Chromatogram_2019-01-16_16-11-35.sky.zip (1 MB)2019-01-161661703401,6501
20160620_Plasma_Exp1_B4_M2_refined_2019-01-16_16-10-19.sky.zip (19 MB)2019-01-16717114269845
MousePlasma_Exp2B7_Chromatogram_2.5x_2019-01-16_16-10-16.sky.zip (929 KB)2019-01-161631603201,5481
MousePlasmaExp2B3_Chromatogram_2019-01-16_16-03-45.sky.zip (305 KB)2019-01-163946924561
20160720_MousePlasmaExp1Batch5_refined_2019-01-16_15-45-10.sky.zip (31 MB)2019-01-161311332661,26045
MousePlasma_Exp2B7_refined_2.5x_2019-01-16_14-55-31.sky.zip (34 MB)2019-01-161591603201,58445
MousePlasma_Exp1B9_Results_refined1_2019-01-16_14-41-33.sky.zip (71 MB)2019-01-161131142281,06239
20190110 - Ajinomoto Complete_2019-01-10_13-31-48.sky.zip (23 MB)2019-01-1163434161238
20180801_UPR_paper_celllines.zip (451 KB)2019-01-10819381369
20180406_180307CN1_Heavy&Light_paper_QExHF02_calcurve3.zip (2 MB)2019-01-108214214427
20180401_180307CN1_Heavy&Light_paper_QExHF02_calcurve1.zip (1 MB)2019-01-108214215027
20180329_180307CN1_Heavy&Light_paper_QExHF02_24hr.zip (558 KB)2019-01-10821421569
PSB33 sustains Photosystem II D1 protein under fluctuating light conditions

  • Organism: Arabidopsis thaliana Col-0
  • Instrument: TSQ vantage Thermo Scientific
  • SpikeIn: No
Abstract
On Earth, solar irradiance varies as the sun rises and sets over the horizon, sunlight is thus in constant fluctuation, following a slow dark-low-high-low-dark curve. Optimal plant growth and development are dependent on the capacity of plants to acclimate and regulate photosynthesis in response to these changes of light. Little is known of regulative processes for photosynthesis during nocturnal events. The nucleus-encoded plant lineage-specific protein PSB33 has been described to stabilize the Photosystem II complex, especially under light stress conditions, and plants lacking PSB33 have a dysfunctional state transition. To clarify the localization and function of this protein, we used phenomic, biochemical and proteomics approaches. We report that PSB33 is predominantly located in non-appressed thylakoid regions and dynamically associates with a thylakoid protein complex in a light-dependent manner. Moreover, under nocturnal periods, plants lacking PSB33 show an accelerated D1 protein degradation, and severely stunted growth when challenged with fluctuating light. We further show that the function of PSB33 precedes the STN7 kinase to regulate/balance the excitation energy of Photosystems I and II in fluctuating light conditions.
Experiment Description
Quantification of the ratio between thylakoids membrane proteins Photosysthem II (PSII), Cytochrome b6f (Cyt b6f) and Photosystem I (PSI) and the kinases STN7 and STN8, the phosphatase TAP38, the proteins PSB33, LHCB1 and LHCB2. Three unique proteotypic peptides from two subunits of PSII (D2 and CP47), PSI (PsaA and PsaB) and Cyt b6f (CytF and CytB6) and from STN7, STN8, TAP38, PSB33, and three shared peptide of LHCB1 and LHCB2 were used to calculate the ratios between the three thylakoid complexes and between the complexes and the abovementioned proteins. Whole thylakoids were denatured in 6M urea 6% acrylamide SDS-PAGE and in-gel digested with trypsin. The peptide mixture has been first analyzed in DDA and the MS/MS acquired utilized to generate the SRM transitions. The sum of the intensities (integrated peak area) of the three most intense transition for every peptide have been utilized to calculate the ratio between protein complexes and targeted proteins.
Sample Description
The ratios have been calculated using wild-type and psb33 knocked-out mutant thylakoid proteins isolated from plants treated with a fluctuating light cycle of 16 hours of Dark, followed by 2 hours of low light, then 2 hours of high light and finally again 2 hours of low light.
Created on 5/3/17, 11:38 AM