Searle_PREGO_manuscript

Analytical protein standards

  • Organism: Human
  • Instrument: TSQ Vantage
  • SpikeIn: No
Abstract
Targeted mass spectrometry is an essential tool for detecting quantitative changes in low abundant proteins throughout the proteome. Although Selected Reaction Monitoring (SRM) is the preferred method for quantifying peptides in complex samples, the process of designing SRM assays is laborious. Peptides have widely varying signal responses dictated by sequence-specific physiochemical properties; one major challenge is in selecting representative peptides to target as a proxy for protein abundance. Here we present PREGO, a software tool that predicts high responding peptides for SRM experiments. PREGO predicts peptide responses with an artificial neural network trained using 11 minimally redundant, maximally relevant properties. Crucial to its success, PREGO is trained using fragment ion intensities of equimolar synthetic peptides extracted from data independent acquisition (DIA) experiments. Due to similarities in instrumentation and the nature of data collection, relative peptide responses from DIA experiments are a suitable substitute for SRM experiments because they both make quantitative measurements from integrated fragment ion chromatograms. Using an SRM experiment containing 12973 peptides from 724 synthetic proteins, PREGO exhibits a 40-85% improvement over previously published approaches at selecting high responding peptides. These results also represent a dramatic improvement over the rules-based peptide selection approaches commonly used in the literature.
Experiment Description
An SRM training validation data set was constructed using the protocols presented in Stergachis et al. Briefly, clones for GST fusion proteins from the pANT7_cGST clone collection (https://dnasu.org/DNASU/Home.do) were synthesized in vitro using the Pierce 1-step Human Coupled in vitro protein synthesis kit (Thermo Scientific; Bremen, Germany). In instances where a cDNA clone was unavailable, recombinant proteins were simply purchased from a commercial source. GST tagged proteins were captured using glutathione sepharose 4B beads (GE Healthcare Life Sciences; Pittsburgh, PA), and iteratively washed to remove nonspecific binders. Bead bound GST fusion proteins were individually denatured with 5 mM dithiothreitol (DTT) for 30 minutes at 60 °C and alkylated with 15 mM iodoacetic acid (IAA) for 30 minutes at room temperature. Proteins were then digested 1 ug of sequencing grade modified porcine trypsin (Promega; Madison, WI) for 2 hours at 37°C.
Created on 4/30/15, 11:44 AM

Library Statistics:

Library Proteins
FileProtein / LabelDescriptionProtein Note/Annotations
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds  
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN Apolipoprotein M OS=Homo sapiens GN=APOM PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN Ceruloplasmin OS=Homo sapiens GN=CP PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN Aspartate aminotransferase, mitochondrial OS=Homo sapiens GN=GOT2 PE=1 SV=3
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN Adenylate kinase isoenzyme 1 OS=Homo sapiens GN=AK1 PE=1 SV=3
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN Prothrombin OS=Homo sapiens GN=F2 PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00738|HPT_HUMAN Haptoglobin OS=Homo sapiens GN=HP PE=1 SV=1
Human_Factor_IX_P00740_with_iRT_092413A_Library_2015-01-30_15-08-31.sky.zipsp|P00740|FA9_HUMAN Coagulation factor IX OS=Homo sapiens GN=F9 PE=1 SV=2
Human_Factor_X_P00742_with_iRT_092413A_Library_2015-01-30_15-07-10.sky.zipsp|P00742|FA10_HUMAN Coagulation factor X OS=Homo sapiens GN=F10 PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00751|CFAB_HUMAN Complement factor B OS=Homo sapiens GN=CFB PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P01008|ANT3_HUMAN Antithrombin-III OS=Homo sapiens GN=SERPINC1 PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P01009|A1AT_HUMAN Alpha-1-antitrypsin OS=Homo sapiens GN=SERPINA1 PE=1 SV=3
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P01011|AACT_HUMAN Alpha-1-antichymotrypsin OS=Homo sapiens GN=SERPINA3 PE=1 SV=2
Human_Angiotensinogen_P01019_Open_Library_Edit_121613_2014-02-18_12-25-38.sky.zipsp|P01019|ANGT_HUMAN Angiotensinogen OS=Homo sapiens GN=AGT PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P01023|A2MG_HUMAN Alpha-2-macroglobulin OS=Homo sapiens GN=A2M PE=1 SV=3
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P01024|CO3_HUMAN Complement C3 OS=Homo sapiens GN=C3 PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P01042-2|KNG1_HUMAN Isoform LMW of Kininogen-1 OS=Homo sapiens GN=KNG1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02647|APOA1_HUMAN Apolipoprotein A-I OS=Homo sapiens GN=APOA1 PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02649|APOE_HUMAN Apolipoprotein E OS=Homo sapiens GN=APOE PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02652|APOA2_HUMAN Apolipoprotein A-II OS=Homo sapiens GN=APOA2 PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02655|APOC2_HUMAN Apolipoprotein C-II OS=Homo sapiens GN=APOC2 PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02656|APOC3_HUMAN Apolipoprotein C-III OS=Homo sapiens GN=APOC3 PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02671|FIBA_HUMAN Fibrinogen alpha chain OS=Homo sapiens GN=FGA PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02675|FIBB_HUMAN Fibrinogen beta chain OS=Homo sapiens GN=FGB PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02741|CRP_HUMAN C-reactive protein OS=Homo sapiens GN=CRP PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02747|C1QC_HUMAN Complement C1q subcomponent subunit C OS=Homo sapiens GN=C1QC PE=1 SV=3
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02749|APOH_HUMAN Beta-2-glycoprotein 1 OS=Homo sapiens GN=APOH PE=1 SV=3
Human_Fibronectin_P02751_Open_Library_edit_08_19_2014_2014-12-08_13-43-11.sky.zipsp|P02751|FINC_HUMAN Fibronectin OS=Homo sapiens GN=FN1 PE=1 SV=4
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02753|RET4_HUMAN Retinol-binding protein 4 OS=Homo sapiens GN=RBP4 PE=1 SV=3
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02763|A1AG1_HUMAN Alpha-1-acid glycoprotein 1 OS=Homo sapiens GN=ORM1 PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02765|FETUA_HUMAN Alpha-2-HS-glycoprotein OS=Homo sapiens GN=AHSG PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02766|TTHY_HUMAN Transthyretin OS=Homo sapiens GN=TTR PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02768|ALBU_HUMAN Serum albumin OS=Homo sapiens GN=ALB PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02774|VTDB_HUMAN Vitamin D-binding protein OS=Homo sapiens GN=GC PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02787|TRFE_HUMAN Serotransferrin OS=Homo sapiens GN=TF PE=1 SV=3
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P02790|HEMO_HUMAN Hemopexin OS=Homo sapiens GN=HPX PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P04004|VTNC_HUMAN Vitronectin OS=Homo sapiens GN=VTN PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P04075|ALDOA_HUMAN Fructose-bisphosphate aldolase A OS=Homo sapiens GN=ALDOA PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P04114|APOB_HUMAN Apolipoprotein B-100 OS=Homo sapiens GN=APOB PE=1 SV=2
LCAT_Template_2015-01-30_15-11-22.sky.zipsp|P04180|LCAT_HUMAN Phosphatidylcholine-sterol acyltransferase OS=Homo sapiens GN=LCAT PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P05090|APOD_HUMAN Apolipoprotein D OS=Homo sapiens GN=APOD PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P05155|IC1_HUMAN Plasma protease C1 inhibitor OS=Homo sapiens GN=SERPING1 PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P06744|G6PI_HUMAN Glucose-6-phosphate isomerase OS=Homo sapiens GN=GPI PE=1 SV=4
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P07195|LDHB_HUMAN L-lactate dehydrogenase B chain OS=Homo sapiens GN=LDHB PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P08697|A2AP_HUMAN Alpha-2-antiplasmin OS=Homo sapiens GN=SERPINF2 PE=1 SV=3
Human_Complement_4_P0C04A_Open_120513_Library_2014-12-10_12-02-42.sky.zipsp|P0C0L5|CO4B_HUMAN Complement C4-B OS=Homo sapiens GN=C4B PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P0DJI8|SAA1_HUMAN Serum amyloid A-1 protein OS=Homo sapiens GN=SAA1 PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P10909|CLUS_HUMAN Clusterin OS=Homo sapiens GN=CLU PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P17174|AATC_HUMAN Aspartate aminotransferase, cytoplasmic OS=Homo sapiens GN=GOT1 PE=1 SV=3
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P19440|GGT1_HUMAN Gamma-glutamyltranspeptidase 1 OS=Homo sapiens GN=GGT1 PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P19652|A1AG2_HUMAN Alpha-1-acid glycoprotein 2 OS=Homo sapiens GN=ORM2 PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P24298|ALAT1_HUMAN Alanine aminotransferase 1 OS=Homo sapiens GN=GPT PE=1 SV=3
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P25311|ZA2G_HUMAN Zinc-alpha-2-glycoprotein OS=Homo sapiens GN=AZGP1 PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P27169|PON1_HUMAN Serum paraoxonase/arylesterase 1 OS=Homo sapiens GN=PON1 PE=1 SV=3
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P35542|SAA4_HUMAN Serum amyloid A-4 protein OS=Homo sapiens GN=SAA4 PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P36269|GGT5_HUMAN Gamma-glutamyltransferase 5 OS=Homo sapiens GN=GGT5 PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P37837|TALDO_HUMAN Transaldolase OS=Homo sapiens GN=TALDO1 PE=1 SV=2
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P37840|SYUA_HUMAN Alpha-synuclein OS=Homo sapiens GN=SNCA PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P40925|MDHC_HUMAN Malate dehydrogenase, cytoplasmic OS=Homo sapiens GN=MDH1 PE=1 SV=4
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P61769|B2MG_HUMAN Beta-2-microglobulin OS=Homo sapiens GN=B2M PE=1 SV=1
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P68871|HBB_HUMAN Hemoglobin subunit beta OS=Homo sapiens GN=HBB PE=1 SV=2
HGA1 and HGG1_library_2014-12-05_17-06-43.sky.zipsp|P69891|HBG1_HUMAN Hemoglobin subunit gamma-1 OS=Homo sapiens GN=HBG1 PE=1 SV=2
HGA1 and HGG1_library_2014-12-05_17-06-43.sky.zipsp|P69905|HBA_HUMAN Hemoglobin subunit alpha OS=Homo sapiens GN=HBA1 PE=1 SV=2
Library Peptides
FileProtein / LabelPeptideStart IndexEnd IndexPeptide Neutral MassMissed CleavagesRT ScorePredicted RTAvg. Measured RTPeptide Note/Annotations
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds AGGSSEPVTGLADK  1287.63070  6.4698
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds DAVTPADFSEWSK  1451.65690  8.1303
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds FLLQFGAQGSPLFK  1551.84490  9.3195
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds GDLDAASYYAPVR  1396.66230  7.7804
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds GTFIIDPAAIVR  1271.72380  8.9976
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds LGGNETQVR  972.49890  5.5566
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds TGFIIDPGGVIR  1243.69250  8.5735
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds TPVISGGPYYER  1337.66160  7.3231
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds TPVITGAPYYER  1365.69290  7.4456
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds VEATFGVDESANK  1365.64120  6.8997
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds YILAGVESNK  1092.58150  7.2285
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN AFLLTPR171178816.4858061.71865530.05007.8950
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN DGLCVPR9198815.3960033.6785724.32006.8635
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN EFPEVHLGQWYFIAGAAPTK37572260.1317096.8102137.23009.2063
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN NQEACELSNN1781881177.467009.42745219.36005.9926
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN SLTSCLDSK1621711009.4750030.52557223.68009.4941
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN TEGRPDMK112120932.43860-18.7932713.59004.9991
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN TELFSSSCPGGIMLNETGQGYQR1201432531.1421074.450232.66008.3786
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN WIYHLTEGSTDLR991121589.7838059.3386229.57007.8315
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN ALYLQYTDETFR69811518.7355073.9289732.55008.8891
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN DDEEFIESNK9479571224.5146034.86263324.56007.2991
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN DIASGLIGPLIICK1872011468.83230100.51781537.99009.9099
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN DIFTGLIGPMK5475581190.6369098.2263437.52009.7928
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN DLYSGLIGPLIVCR8879011574.84910108.09888539.540010.1783
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN EFVVMFSVVDENFSWYLEDNIK2152372709.26730126.8470643.370010.9475
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN EVGPTNADPVCLAK5235371469.7184042.52327326.13007.6262
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN EYTDASFTNR4264361202.5204029.7750123.52007.1325
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN GAYPLSIEPIGVR4684811370.7558075.54767632.88008.9385
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN GEFYIGSK770778899.4389043.6594726.36007.6592
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN GPEEEHLGILGPVIWAEVGDTIR4394622486.28050111.3750140.210010.3470
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN GSLHANGR562570810.40960-31.87229710.92004.6921
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN GVYSSDVFDIFPGTYQTLEMFPR100810312668.25190-147.05215-12.630010.7683
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN IYHSHIDAPK1771871179.603704.85469118.43006.1385
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN LISVDTEHSNIYLQNGPDR43622170.0655051.57700727.98007.9867
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN MHAINGR957964797.39660-7.915449615.82005.6564
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN MHSMNGFMYGNQPGLTMCK6196382202.9141060.9661529.90008.3531
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN MYSVNGYTFGSLPGLSMCAEDR2582802454.0654094.7364936.80009.6951
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN MYYSAVDPTK5375471173.5376037.80349725.16007.4409
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN NNEGTYYSPNYNPQSR4845001902.8133031.73248123.92007.1965
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN QSEDSTFYLGER7207321430.6314052.8735228.24008.0351
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN QYTDSTFR7857931016.4563022.9405822.12006.8533
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN SGAGTEDSACIPWAYYSTVDQVK8648872504.11660-58.5764165.46009.2220
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN SVPPSASHVAPTETFTYEWTVPK5005232530.2380072.54426632.27008.8000
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN TTIEKPVWLGFLGPIIK81981911.12340109.8100239.890010.3189
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN TYCSEPEK2372451012.41720-6.039060616.20005.7345
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN TYSDHPEK936944975.42980-16.30909314.10005.3183
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN TYYIAAVEVEWDYSPQR7327492088.9793092.4120836.33009.5664
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN YTVNQCR712719939.423300.3191003817.50005.9761
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN ASAELALGENSEVLK1071221529.7937056.65674229.02007.6454
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN DAGMQLQGYR1701801137.5237038.4796525.30006.9458
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN DDNGKPYVLPSVR68811458.7467043.9292526.42007.1419
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN EFSIYMTK3964041017.4841061.78222730.07007.8554
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN EIATVVK227234758.4538019.14743621.35006.1950
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN EYLPIGGLAEFCK941071495.7381088.64303635.56008.8861
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN FVTVQTISGTGALR1251391448.7987061.26437429.96007.8103
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN HFIEQGINVCLCQSYAK2622792065.9714057.5151529.19007.6955
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN IAAAILNTPDLR3253371266.7296064.6261530.65007.9705
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN IGASFLQR139147890.4974050.60225727.78007.4031
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN MNLGVGAYR5968979.4909047.22941627.09007.2802
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN NMGLYGER279287938.4280034.70211424.53006.7997
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN QWLQEVK338345929.4971048.91470727.44006.8261
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN TCGFDFTGAVEDISK1852001645.7294082.8366434.37008.6734
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN TQLVSNLK355363901.5233029.15268123.39006.5803
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN VGAFTMVCK2872961011.4882049.5213227.56007.3819
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN ATEPVIAFYEK1551661266.6496068.0459931.35009.3558
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN EVQQGEEFER971071249.5575018.21270421.16007.4090
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN GFLIDGYPR88971036.5342070.18606631.78009.4306
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN GQLVPLETVLDMLR63771582.87530120.27942.030011.5274
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN IGQPTLLLYVDAGPETMTQR1081282202.1355092.1276236.270010.3249
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN IIFVVGGPGSGK9211129.6495065.3818730.80009.2291
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN LSEIMEK5663848.4314025.08728822.56007.6562
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN SEVSSGSAR4453878.40940-31.7693110.94005.2761
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN VDDNEETIK1381471061.487702.3042217.91006.8140
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN VNAEGSVDSVFSQVCTHLDALK1721942375.1427092.7659936.400010.2906
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN YGYTHLSTGDLLR31441494.7467056.77402529.04008.8872
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN ELLESYIDGR3533631193.59280  8.0462
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN ETAASLLQAGYK4864981250.65070  7.5667
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN ETWTANVGK5085171004.49270  6.6940
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN GDACEGDSGGPFVMK5605751525.61770  7.3179
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN GQPSVLQVVNLPIVERPVCK5175372231.24600  8.6405
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN GQQYQGR217224835.39370  2.7094
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN HQDFNSAVQLVENFCR2472631962.90060  8.6351
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN ITDNMFCAGYKPDEGK5435591844.80730  7.2379
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN IVEGSDAEIGMSPWQVMLFR3633832264.09700  9.8924
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN KPVAFSDYIHPVCLPDR4694862013.01420  7.9749
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN LAACLEGNCAEGLGTNYR991171967.88290  7.3804
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN LAVTTHGLPCLAWASAQAK2242431994.04080  8.2753
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN NFTENDLLVR4154251219.61970  7.8575
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN NPDSSTTGPWCYTTDPTVR1581772153.93240  7.7363
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN QECSIPVCGQDQVTVAMTPR1781982275.03950  7.6160
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN SEGSSVNLSPPLEQCVPDR1982172069.96880  7.6120
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN SGIECQLWR1241331147.54440  7.7948
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN SPQELLCGASLISDR3843991644.81410  8.3663
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN TATSEYQTFFNPR3143271560.72090  7.9978
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN TFGSGEADCGLRPLFEK3273441882.88830  7.9600
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN VIDQFGE615622806.38100  7.3658
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN VTGWGNLK500508873.47090  7.1956
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN WYQMGIVSWGEGCDR5815961842.78180  8.9060
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN YGFYTHVFR5996081188.57160  7.6512
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN YTACETAR8694970.41780  5.2325
Library Precursors
FileProtein / LabelPrecursorQ1 ZLabelQ1 m/zPrecursor Neutral MassCEDPPrecursor Note/Annotations
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds AGGSSEPVTGLADK2+light644.82261287.630722.2497 
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds DAVTPADFSEWSK2+light726.83571451.656924.7101 
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds FLLQFGAQGSPLFK2+light776.92981551.844926.2129 
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds GDLDAASYYAPVR2+light699.33841396.662323.8852 
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds GTFIIDPAAIVR2+light636.86921271.723822.0111 
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds LGGNETQVR2+light487.2567972.498917.5227 
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds TGFIIDPGGVIR2+light622.85351243.692521.5906 
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds TPVISGGPYYER2+light669.83811337.661623.0001 
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds TPVITGAPYYER2+light683.85371365.692923.4206 
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds VEATFGVDESANK2+light683.82791365.641223.4198 
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zipiRT_Stds YILAGVESNK2+light547.29801092.581519.3239 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN AFLLTPR2+light409.2502816.485815.1825 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN DGLCVPR2+light408.7053815.396015.1662 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN EFPEVHLGQWYFIAGAAPTK2+light1131.07312260.131736.8372 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN NQEACELSNN2+light589.74081177.467020.5972 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN SLTSCLDSK2+light505.74481009.475018.0773 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN TEGRPDMK2+light467.2266932.438616.9218 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN TELFSSSCPGGIMLNETGQGYQR2+light1266.57832531.142140.9023 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|O95445|APOM_HUMAN WIYHLTEGSTDLR2+light795.89921589.783826.7820 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN ALYLQYTDETFR2+light760.37501518.735525.7162 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN DDEEFIESNK2+light613.26461224.514621.3029 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN DIASGLIGPLIICK2+light735.42341468.832324.9677 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN DIFTGLIGPMK2+light596.32571190.636920.7948 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN DLYSGLIGPLIVCR2+light788.43181574.849126.5580 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN EFVVMFSVVDENFSWYLEDNIK2+light1355.64092709.267343.5742 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN EVGPTNADPVCLAK2+light735.86651469.718424.9810 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN EYTDASFTNR2+light602.26751202.520420.9730 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN GAYPLSIEPIGVR2+light686.38521370.755823.4966 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN GEFYIGSK2+light450.7267899.438916.4268 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN GPEEEHLGILGPVIWAEVGDTIR2+light1244.14752486.280540.2294 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN GSLHANGR2+light406.2121810.409615.0914 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN GVYSSDVFDIFPGTYQTLEMFPR2+light1335.13322668.251942.9590 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN IYHSHIDAPK2+light590.80911179.603720.6293 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN LISVDTEHSNIYLQNGPDR2+light1086.04002170.065535.4862 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN MHAINGR2+light399.7056797.396614.8962 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN MHSMNGFMYGNQPGLTMCK2+light1102.46432202.914135.9789 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN MYSVNGYTFGSLPGLSMCAEDR2+light1228.04002454.065439.7462 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN MYYSAVDPTK2+light587.77611173.537620.5383 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN NNEGTYYSPNYNPQSR2+light952.41391902.813331.4774 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN QSEDSTFYLGER2+light716.32301430.631424.3947 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN QYTDSTFR2+light509.23541016.456318.1821 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN SGAGTEDSACIPWAYYSTVDQVK2+light1253.06562504.116640.4970 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN SVPPSASHVAPTETFTYEWTVPK2+light1266.12632530.238040.8888 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN TTIEKPVWLGFLGPIIK2+light956.56901911.123431.6021 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN TYCSEPEK2+light507.21591012.417218.1215 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN TYSDHPEK2+light488.7222975.429817.5667 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN TYYIAAVEVEWDYSPQR2+light1045.49692088.979334.2699 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00450|CERU_HUMAN YTVNQCR2+light470.7189939.423317.0266 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN ASAELALGENSEVLK2+light765.90411529.793725.8821 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN DAGMQLQGYR2+light569.76911137.523719.9981 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN DDNGKPYVLPSVR2+light730.38061458.746724.8164 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN EFSIYMTK2+light509.74931017.484118.1975 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN EIATVVK2+light380.2342758.453814.3120 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN EYLPIGGLAEFCK2+light748.87631495.738125.3713 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN FVTVQTISGTGALR2+light725.40661448.798724.6672 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN HFIEQGINVCLCQSYAK2+light1033.99302065.971433.9248 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN IAAAILNTPDLR2+light634.37211266.729621.9362 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN IGASFLQR2+light446.2560890.497416.2927 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN MNLGVGAYR2+light490.7527979.490917.6276 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN NMGLYGER2+light470.2213938.428017.0116 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN QWLQEVK2+light465.7558929.497116.8777 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN TCGFDFTGAVEDISK2+light823.87201645.729427.6212 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN TQLVSNLK2+light451.7689901.523316.4581 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00505|AATM_HUMAN VGAFTMVCK2+light506.75141011.488218.1075 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN ATEPVIAFYEK2+light634.33211266.649621.9350 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN EVQQGEEFER2+light625.78601249.557521.6786 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN GFLIDGYPR2+light519.27441036.534218.4832 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN GQLVPLETVLDMLR2+light792.44491582.875326.6783 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN IGQPTLLLYVDAGPETMTQR2+light1102.07502202.135535.9673 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN IIFVVGGPGSGK2+light565.83201129.649519.8800 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN LSEIMEK2+light425.2230848.431415.6617 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN SEVSSGSAR2+light440.2120878.409416.1114 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN VDDNEETIK2+light531.75111061.487718.8575 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN VNAEGSVDSVFSQVCTHLDALK2+light1188.57862375.142738.5624 
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zipsp|P00568|KAD1_HUMAN YGYTHLSTGDLLR2+light748.38061494.746725.3564 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN ELLESYIDGR2+light597.80371193.592820.8391 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN ETAASLLQAGYK2+light626.33261250.650721.6950 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN ETWTANVGK2+light503.25361004.492718.0026 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN GDACEGDSGGPFVMK2+light763.81611525.617725.8195 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN GQPSVLQVVNLPIVERPVCK2+light1116.63032231.246036.4039 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN GQQYQGR2+light418.7041835.393715.4661 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN HQDFNSAVQLVENFCR2+light982.45761962.900632.3787 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN ITDNMFCAGYKPDEGK2+light923.41091844.807330.6073 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN IVEGSDAEIGMSPWQVMLFR2+light1133.05582264.097036.8967 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN KPVAFSDYIHPVCLPDR2+light1007.51442013.014233.1304 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN LAACLEGNCAEGLGTNYR2+light984.94871967.882932.4535 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN LAVTTHGLPCLAWASAQAK2+light998.02771994.040832.8458 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN NFTENDLLVR2+light610.81711219.619721.2295 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN NPDSSTTGPWCYTTDPTVR2+light1077.97352153.932435.2442 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN QECSIPVCGQDQVTVAMTPR2+light1138.52702275.039537.0608 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN SEGSSVNLSPPLEQCVPDR2+light1035.99172069.968833.9847 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN SGIECQLWR2+light574.77951147.544420.1484 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN SPQELLCGASLISDR2+light823.41431644.814127.6074 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN TATSEYQTFFNPR2+light781.36771560.720926.3460 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN TFGSGEADCGLRPLFEK2+light942.45151882.888331.1785 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN VIDQFGE2+light404.1978806.381015.0309 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN VTGWGNLK2+light437.7427873.470916.0373 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN WYQMGIVSWGEGCDR2+light922.39821842.781830.5769 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN YGFYTHVFR2+light595.29311188.571620.7638 
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zipsp|P00734|THRB_HUMAN YTACETAR2+light486.2162970.417817.4915 
Clustergrammer Heatmap
Flag FileDownloadCreatedProteinsPeptidesPrecursorsTransitionsReplicates
Human_Prothrombin_P00734_Open_Edit_with_iRT_121213_2015-01-30_17-04-21.sky.zip2015-04-30125252051
LCAT_Template_2015-01-30_15-11-22.sky.zip2015-04-30188631
Human_Factor_IX_P00740_with_iRT_092413A_Library_2015-01-30_15-08-31.sky.zip2015-04-3011212891
Human_Factor_X_P00742_with_iRT_092413A_Library_2015-01-30_15-07-10.sky.zip2015-04-30113131031
Human_Complement_4_P0C04A_Open_120513_Library_2014-12-10_12-02-42.sky.zip2015-04-30148483212
Human_Fibronectin_P02751_Open_Library_edit_08_19_2014_2014-12-08_13-43-11.sky.zip2015-04-30154543831
HGA1 and HGG1_library_2014-12-05_17-06-43.sky.zip2015-04-30288701
Chromatogram_Library_08_19_2014_Build_DBS_Targets_2014-09-02_14-27-37.sky.zip2015-04-30541,0321,0327,37453
Human_ORM1_P02763_Open_Library_edit_08_19_2014_2014-08-22_16-21-09.sky.zip2015-04-3011010761
Human_APOB100_P04114_Open_Edit_with_iRT_120913_Figures_2014-06-09_11-19-51.sky.zip2015-04-3022022027602
Human_Angiotensinogen_P01019_Open_Library_Edit_121613_2014-02-18_12-25-38.sky.zip2015-04-30114141271
Human_GPT1_P24298_Wide_Open_120213_2013-12-06_15-59-13.zip2015-04-3011313901
Human_ALDOA_P04075_Open_112713_2013-12-06_15-53-56.zip2015-04-30117171181
Human_A2M_P01023_Open_120513_2013-12-06_15-51-36.zip2015-04-30163635271