System Suitability

System Suitability
Mass calibration

positive mode: passed

-LBC
2024-06-26 10:30:002024-06-26 10:30:00Instrumentation ChangeExploris_PSB2024-06-26 07:43:012024-06-26 07:43:01
Mass calibration

positive mode: passed

-LBC
2024-06-24 11:00:002024-06-24 11:30:00Instrumentation ChangeExploris_PSB2024-06-24 08:37:492024-06-24 08:37:49
Mass calibration

positive mode: passed

-LBC
2024-06-18 00:00:002024-06-18 00:00:00Instrumentation ChangeExploris_PSB2024-06-18 08:27:192024-06-18 08:27:19
Full system calibration.

positive mode: passed
negative mode: passed

-LBC
2024-06-03 00:00:002024-06-03 00:00:00Instrumentation ChangeExploris_PSB2024-06-03 09:39:232024-06-03 09:39:23
Mass calibration

positive mode: passed
negative mode: passed

-LBC
2024-02-13 00:00:002024-02-13 00:00:00Instrumentation ChangeExploris_PSB2024-02-13 07:13:162024-02-13 07:13:16
Mass and system calibration

positive mode: passed

-LBC
2024-02-06 00:00:002024-02-06 00:00:00Instrumentation ChangeExploris_PSB2024-02-06 10:22:012024-02-06 10:22:01
Mass axis calibration.
positive mode: passed
negative mode: passed

-LBC
2023-10-16 00:00:002023-10-16 00:00:00Instrumentation ChangeExploris_PSB2023-10-16 08:52:342023-10-16 08:52:34
Mass axis calibration
pos mode: passed
neg mode: passed

-LBC
2023-10-12 00:00:002023-10-12 00:00:00Instrumentation ChangeExploris_PSB2023-10-12 13:17:122023-10-12 13:17:12
Mass calibration.
positive mode: passed
negative mode: passed

LBC
2023-08-09 12:00:002023-08-10 00:00:00Instrumentation ChangeExploris_PSB2023-08-09 08:57:492023-08-09 08:57:49
Orbitrap mass accuracy check.

positive mode: passed

LBC
2023-06-30 14:30:00 Instrumentation ChangeEclipse_Polk2023-06-30 11:29:442023-06-30 11:29:44
New BSA and HeLa are used2023-06-21 00:00:00 <774>Eclipse_PSB2023-06-21 05:36:282023-06-21 05:36:57
Analytical column change2023-06-21 00:00:00 <756>Eclipse_PSB2023-06-21 09:42:332023-06-21 10:08:07
Full system and mass calibration.
negative mode: passed

Mass calibration only.
positive mode: passed
2023-05-31 19:00:00 Instrumentation ChangeEclipse_Polk2023-05-31 16:04:042023-05-31 16:04:04
New column and trap installed.

Analytical Column: ES75500PN
S/N: C20420285
Installed on May 15, 2023
2023-05-15 00:00:00 <756>Eclipse_PSB2023-05-17 06:07:232023-05-17 06:07:23
Fresh MPA, MPB, IPA solvents.2023-05-15 00:00:00 <757>Eclipse_PSB2023-05-17 06:08:272023-05-17 06:08:27
New trap column and trap installed.

Trap Column: 174500
Lot # 01274561
Installed on May 11, 2023
2023-05-11 00:00:00 <756>Eclipse_PSB2023-05-17 06:06:422023-05-17 06:06:42
Full calibration2023-05-10 00:00:00 Instrumentation ChangeEclipse_PSB2023-05-17 06:10:322023-05-17 06:10:32
Cleaned ion funnel, lens stack, and exit lens of bent flatapole.

-LBC
2023-05-04 11:00:00 Instrumentation ChangeExploris_PSB2023-05-04 08:15:372023-05-04 08:15:37
Full system calibration by John Polese.
positive mode: passed
negative mode: passed
2023-04-14 15:00:00 Instrumentation ChangeEclipse_Polk2023-04-18 06:40:402023-04-18 06:40:40
Annual PM by John Polese.2023-04-11 09:00:00 Instrumentation ChangeEclipse_Polk2023-04-18 06:39:112023-04-18 06:39:11
Full system calibrations performed.
positive mode: passed
negative mode: passed

LBC
2023-04-04 12:00:00 Instrumentation ChangeEclipse_Polk2023-04-04 09:28:152023-04-04 09:28:15
Cleaned ion funnel lens stack. Began pumpdown and orbi bakeout.

TIW, LBC
2023-04-03 09:00:00 Instrumentation ChangeEclipse_Polk2023-04-04 09:27:102023-04-04 09:27:10
Mass and system calibration
pos mode: passed
neg mode: passed

LBC
2023-03-24 16:00:00 Instrumentation ChangeExploris_PSB2023-03-24 13:21:212023-03-24 13:21:21
Trap column: PepMap 100 75um x 2cm C18
SN 20344516

Analytical column: PepMap RSLC C18 75um x 25cm
SN C20415505
2023-03-24 15:00:00 Instrumentation ChangeExploris_PSB2023-03-24 13:23:292023-03-24 13:23:29
Mass axis calibratriuon
positive mode: passed
negative mode: passed

LBC
2022-11-14 13:00:00 Instrumentation ChangeExploris_PSB2022-11-15 06:37:462022-11-15 06:37:46
Mass axis calibration
positive mode: passed
negative mode: passed

LBC
2022-10-31 11:00:00 Instrumentation ChangeExploris_PSB2022-10-31 10:19:202022-10-31 10:19:20
Mass spectrometer calibration
system calibration positive mode: pass
system calibration negative mode: pass

LBC
2022-10-28 13:00:00 Instrumentation ChangeExploris_PSB2022-10-28 10:41:142022-10-28 10:41:14
Calibrated mass axis.
positive mode passed.
negative mode passed.

LBC
2022-09-14 08:30:00 Instrumentation ChangeExploris_Dabney2022-09-14 05:27:552022-09-14 05:27:55
Changed trap and anlytical columns.

Acclaim PepMap 100: SN 20349855
PepMap RSLC C18: SN C20298282

LBC
2022-09-14 08:30:00 <366>Exploris_Dabney2022-09-14 05:30:182022-09-14 05:30:18
Full calibration performed by Leonard Collins.
positive mode passed.
Negative mode passed.

Set up Easy nLC-1200 for system evaluation.

LBC
2022-09-12 12:00:00 Instrumentation ChangeExploris_Dabney2022-09-13 05:16:522022-09-13 05:16:52
Full cleaning performed by Leonard Collins.
Cleaned ion funnel, entrance quad, and isolation quad.
White residue on all 4 rods of isolation quad that required hard wiping with wet cloth.

LBC
2022-09-08 12:00:00 Instrumentation ChangeExploris_Dabney2022-09-13 05:15:482022-09-13 05:15:48
Installed analytical column
PepMap RSLC C18
2um 100A 75um x 25cm
Part No. ES902
SN C20298278

LBC
2022-07-28 17:30:00 <383>Exploris_PSB2022-07-28 14:23:172022-07-28 14:23:17
positive mode mass calibration passed.
negative mode mass calibration passed.

LBC
2022-07-28 16:45:00 Instrumentation ChangeExploris_PSB2022-07-28 13:49:322022-07-28 13:49:32
Changed column2022-07-28 00:00:00 Instrumentation ChangeJRE OE240 PFAS2022-07-28 11:51:132022-07-28 11:51:13
MA10084N was moved from Toxicology bldg to Plant Sciences, room 2354.

Diagnostics for current status were performed on 6/7/22. All tests and calibrations passed.

Instrument was installed and powered up by Leonard Collins on 7/22/22.

Instrument was calibrated in positive and negative mode and ion optics tests performed on 7/25/22. All calibrations and tests passed.

Relevant documents uploaded to Mendix.
2022-07-25 12:30:00 Instrumentation ChangeExploris_PSB2022-07-25 09:30:432022-07-25 09:30:43
Removed old trap column. Connected new Acclaim PepMap 100, 75 um x 2 cm C18, 3 um particle.

SN: 20346011

Prepared new mobile phases and IPA/water wash solvent.
2022-07-25 12:30:00 <383>Exploris_PSB2022-07-25 09:32:312022-07-25 09:32:31
Analytical column replaced.
SN: C20298260
LBC
2022-04-26 13:00:00 <383>Exploris_PSB2022-04-26 11:08:072022-04-26 11:08:07
Mass axis calibrated.
positive mode: success
negative mode: success
LBC
2022-04-26 12:00:00 Instrumentation ChangeExploris_PSB2022-04-26 11:09:032022-04-26 11:09:03
System taken down to clean quadrupole.2022-04-01 00:00:00 <1011>Figure 2 System Suitability2024-08-16 15:46:282024-08-16 15:46:28
Replaced Pump S buffer.2022-03-21 22:41:48 <1010>Figure 2 System Suitability2024-08-16 15:46:282024-08-16 15:46:28
LC issue - speculate related to Pump S buffer?2022-03-21 01:25:57 <1010>Figure 2 System Suitability2024-08-16 15:46:282024-08-16 15:46:28
New operator. 1 ug injection of plasma-derived particles.2022-03-13 10:11:56 Technician ChangeFigure 2 System Suitability2024-08-16 15:46:282024-08-16 15:46:28
Swapped to PepSep column.2022-03-13 10:11:56 <1012>Figure 2 System Suitability2024-08-16 15:46:282024-08-16 15:46:28
Mass axis calibration
positive mode: passed
negative mode: passed
LBC
2022-03-07 11:00:00 Instrumentation ChangeExploris_Dabney2022-03-07 07:59:142022-03-07 07:59:14
Mass axis calibration
positive mode: passed
negative mode: passed
LBC
2022-02-24 15:00:00 Instrumentation ChangeExploris_Dabney2022-02-24 12:05:442022-02-24 12:05:44
Mass and system calibration (Pos and Neg)
Dab 480

YCC
2022-02-16 00:00:00 Instrumentation ChangeExploris_Dabney2022-02-18 06:58:122022-02-18 06:58:40
Analytical column changed2022-01-17 00:00:00 <366>Exploris_Dabney2022-01-20 06:55:292022-01-20 06:55:29
Full calibration:
positive mode: passed

Mass calibration:
negative mode: passed

Analytical column was plugged. Sonicated for a very long time. It "might"be fixed. Need to run HeLa.

LBC
2021-12-29 16:00:00 Instrumentation ChangeExploris_Dabney2021-12-29 12:54:182021-12-29 12:59:30
Because the flow was shut off and the column not properly prepped for storage during the time it took for Thermo to make the pump repair, the analytical column became plugged, likely due to evaporation at the tip leaving an insoluble deposit. We should create a short procedure to flush the column before we have Thermo engineers make repairs, since we do not know exactly how long the downtime will be.

New Trap: SN 20229892
New column: SN C20294372

LBC
2021-12-10 11:00:00 <366>Exploris_Dabney2021-12-10 07:48:242021-12-10 07:48:24
Full calibration
positive mode: passed
negative mode: passed

LBC
2021-12-08 10:00:00 Instrumentation ChangeExploris_Dabney2021-12-10 07:50:142021-12-10 07:50:14
Oil rough pumps removed.
Dry rough pump installed: nXL 110i Dual Inlet
John Polese
2021-12-07 09:00:00 <365>Exploris_Dabney2021-12-08 10:27:202021-12-10 07:50:22
Engineer replaced Pump B on the easyNano.

LBC
2021-12-06 09:00:00 <365>Exploris_Dabney2021-12-10 07:51:342021-12-10 07:51:34
Trap column changed SN 2022298662021-11-16 00:00:00 <366>Exploris_Dabney2021-11-16 14:57:152021-11-16 14:58:24
Mass axis calibration:
positive mode passed
negative mode passed
LBC
2021-10-28 15:30:00 Instrumentation ChangeExploris_Dabney2021-10-28 12:29:232021-10-28 12:29:23
Trap column replaced:
75um x 2cm C18 3um Acclaim PepMap 100
SN 20211630
LBC
2021-10-22 10:45:00 <383>Exploris_PSB2021-10-22 07:49:012021-10-22 07:49:01
Full calibration: positive mode passed
Mass Cal only: negative mode passed
LBC
2021-10-22 10:30:00 Instrumentation ChangeExploris_PSB2021-10-22 07:47:142021-10-22 07:47:14
Fresh the solvents2021-10-22 00:00:00 Reagent ChangeExploris_Dabney2021-11-16 14:56:242021-11-16 14:56:24
Both analytical and trap columns are changed.

Analytical SN C20216694
Trap SN 20229891

HB
2021-10-14 00:00:00 <366>Exploris_Dabney2021-11-16 14:55:312021-11-16 14:58:15
Change new MP A and MP B2021-09-29 00:00:00 <384>Exploris_PSB2021-09-29 10:17:312021-09-29 10:17:31
Posiive mode mass cal and system cal

YCC
2021-09-29 00:00:00 Instrumentation ChangeExploris_PSB2021-09-29 10:22:242021-09-29 10:22:24
MPA and MPB were refreshed in the nanoLC.
--Taufika Islam Williams
2021-09-17 13:00:00 Reagent ChangeExploris_Dabney2021-09-23 12:38:402021-09-23 12:38:40
Mass axis calibration:
positive mode passed
negative mode passed
LBC
2021-09-17 09:30:00 Instrumentation ChangeExploris_Dabney2021-09-17 06:42:332021-09-17 06:42:33
Mass axis calibration:
positive mode passed
negative mode passed
LBC
2021-09-13 13:00:00 Instrumentation ChangeExploris_Dabney2021-09-13 10:16:382021-09-13 10:16:38
Started running 20 min NEW METHOD2021-09-01 05:00:00 Instrumentation ChangeJRE OE240 PFAS2021-09-03 07:58:452021-09-03 07:59:33
Mass axis calibration:
positive mode passed
negative mode passed
LBC
2021-08-24 14:30:00 Instrumentation ChangeExploris_PSB2021-08-24 11:37:252021-08-24 11:37:25
Full calibration:
positive mode passed
negative mode passed
2021-08-24 08:30:00 Instrumentation ChangeExploris_Dabney2021-08-24 06:44:592021-08-24 06:44:59
Mass Calibration2021-08-20 00:00:00 Instrumentation ChangeExploris_Dabney2021-08-20 07:16:482021-08-20 07:16:48
Easy-Spray temperature control cable was replaced today.
Production Order: 0247632.
LC167 Revision B.
Jon Delgado from Thermo was onsite and looked at it. He will order a new one for us.
--TIW
2021-07-27 13:30:00 Instrumentation ChangeExploris_Dabney2021-07-27 10:46:402021-07-27 10:46:57
Mass Calibration: +ve and -ve.
--Taufika Islam Williams
2021-07-27 11:30:00 Instrumentation ChangeExploris_Dabney2021-07-27 08:34:302021-07-27 08:34:30
Full calibration.
positive mode: passed
negative mode: passed
LBC
2021-07-22 14:00:00 Instrumentation ChangeExploris_Dabney2021-07-23 07:53:012021-07-23 07:53:01
Both analytical and trap columns were changed in the morning of 7/14/21 by Hellena Bai.

Analytical column SN: C20151956
Trap column SN: 20211631

Easy Spray Temperature Control cable changed.

Last columns have been resting for a while
2021-07-14 00:00:00 <383>Exploris_PSB2021-07-14 11:31:462021-07-27 13:45:59
-Mass calibration in (+) and (-) mode, both are passed quickly
-solvent change
2021-07-14 00:00:00 Instrumentation ChangeExploris_PSB2021-07-14 11:34:282021-07-14 11:34:28
Trap column replaced.
Acclaim Pepmap 100, 75um x 2 cm
SN 20229892
2021-07-12 16:00:00 <366>Exploris_Dabney2021-07-12 14:05:282021-07-12 14:05:28
Mass axis calibration:
positive: passed
negative: passed
LBC
2021-07-12 13:00:00 Instrumentation ChangeExploris_Dabney2021-07-12 14:04:112021-07-12 14:04:11
-Tune software update
-Quad clean
-Full calibration
----------
Jon Delgado
Field Service Engineer III
Unity Lab Services
Thermo Fisher Scientific
Customer Service: +1 (800) 532-4752
Jon.delgado@thermofisher.com | www.unitylabservices.com
2021-07-07 00:00:00 Instrumentation ChangeExploris_PSB2021-07-08 17:32:202021-07-08 17:32:28
Mass Calibration +ve and -ve.
--TIW
2021-07-02 11:30:00 Instrumentation ChangeExploris_Dabney2021-07-27 08:33:492021-07-27 08:33:49
Instrument functioning as intended. Ran evaluations prior to instrument vent, failed for quadrupole calibrations. Charging
present in bent flatapole and quad based on diagnostics. Customer wanted performance maintenance training also.
Vented instrument and showed customer how to clean optics, bent flatapole and quadrupole. Set 10 hour bakeout.
Instrument got down to 6e-10. Had left vacuum override on and errors appeared after reset. Instrument vacuum changed
to e-12 for UHV. Did multipole resets with no success. UHV gauge backordered. Vented instrument and inspected
vacuum hose. No issues. Started pump down on instrument. UHV started at e-7 and continued going down. Once at e-8, set 14 hour bakeout. Next day came onsite. vacuum at e-10. Reenabled vacuum controls and issues with ion optics and
instrument control board reappeared. Reset pc and deleted and reinstalled tune software.

-Jon D
2021-06-25 00:00:00 <540>Polk_Exploris2021-06-29 08:51:272021-06-29 09:12:26
Quad clean.
Full calibration.
-Jon Delgado (FSE)
2021-06-23 00:00:00 <365>Exploris_Dabney2021-06-25 05:09:402021-06-25 05:09:40
New Analytical Column2021-06-08 08:00:00 Instrumentation ChangeJRE OE240 PFAS2021-06-30 11:49:262021-06-30 11:49:26
I swapped computers and managed to swap the d drives so all your data is in the new computer.
I upgraded you all to Tune 3.0 and set the vnc connection for the EasyNano which is actively working.
I'll take back the old computer to RMA it.
You all will have to set up your IT to come in and map this to your network so you all can remote in.
I'm currently running through system checks and will give you another update in an hour or so.
Instrument passed both positive and negative checks and I see no issues with the instrument. When one of you has a moment, could we start an injection just to make sure there are no issues with the MS triggering?

Jon Delgado
Field Service Engineer III
2021-06-07 00:00:00 <365>Exploris_Dabney2021-06-07 13:06:202021-06-07 13:13:33
Alllison Stewart opened a ticket and will let us know when an IP address has been assigned to the new computer.
Computer name: JZ3NB93; User name: MA10095N; PWD: Thermo.
Once the new computer has internet access, we will start a QC sequence.
DinoLite software will also need to be installed.

--TIW
2021-06-07 00:00:00 Instrumentation ChangeExploris_Dabney2021-06-07 13:12:432021-06-07 13:13:15
Mass calibration (+ve and -ve).
Changed HCTT (high capacity transfer tube).
--TIW
2021-06-03 00:00:00 Instrumentation ChangeExploris_PSB2021-06-03 12:51:042021-06-03 12:51:21
Clean quadrupole and hexapole cartridge2021-05-29 00:00:00 Instrumentation ChangeImpact-II weekly QC2021-05-31 08:24:512021-05-31 08:24:51
Cleaning of glass capillary2021-05-28 00:00:00 Instrumentation ChangeImpact-II weekly QC2021-05-28 21:52:322021-05-28 21:52:32
Mass calibration: positive
Mass calibration: negative
LBC
2021-05-04 10:00:00 Instrumentation ChangeExploris_PSB2021-05-04 07:08:382021-05-04 07:08:38
Installed analytical column: SN B20132421
LBC
Last column quickly developed clogged tip.
2021-04-29 12:00:00 <383>Exploris_PSB2021-04-29 09:46:212021-04-29 09:46:21
Mass calibration: positive
Mass calibration: negative
LBC
2021-04-29 00:00:00 Instrumentation ChangeExploris_PSB2021-04-29 11:16:072021-04-29 11:16:07
Installed analytical column: SN B20088703
Installed trap column: SN 20078257
LBC
2021-04-19 15:00:00 <383>Exploris_PSB2021-04-19 12:20:252021-04-19 12:20:25
Full calibration: positive
Full calibration: negative
LBC
2021-04-19 15:00:00 Instrumentation ChangeExploris_PSB2021-04-20 13:01:522021-04-20 13:01:52
Full calibration of MS (+ve & -ve)
Clean HCTT.
--TIW
2021-04-15 00:00:00 Instrumentation ChangeExploris_Dabney2021-04-15 12:16:002021-04-15 12:19:00
New trap column
New analytical column.
--TIW
2021-04-15 00:00:00 <366>Exploris_Dabney2021-04-15 12:17:112021-04-15 12:19:08
Fresh nanoLC solvents (MPA & MPB)
Performed leak test
Checked flow sensor calibration.
--TIW
2021-04-15 00:00:00 Reagent ChangeExploris_Dabney2021-04-15 12:18:242021-04-15 12:18:51
new PicoTip emitter (New Objective, Stock #: FS360-50-15-D-20, DOM: 12-04-2018, Coating: 1P-4P, Tip: 15+/-1um)2021-04-15 00:00:00 Instrumentation ChangeImpact-II weekly QC2021-04-16 06:55:092021-04-16 06:55:09
Mass calibration: positive
Mass calibration: negative
LBC
2021-04-09 10:00:00 Instrumentation ChangeExploris_Dabney2021-04-09 07:14:412021-04-09 07:14:41
Mass calibration: positive
Mass calibration: negative
LBC
2021-03-31 11:00:00 Instrumentation ChangeExploris_PSB2021-03-31 07:43:382021-03-31 07:43:38
New trap and analytical columns installed:
Trap: Acclaim PepMap 100; SN 20211652
Analytical: PepMap RSLC C18; SN B20137165
2021-03-31 10:00:00 <383>Exploris_PSB2021-03-31 07:23:572021-03-31 07:23:57
Mass calibration (positive and negative).2021-03-29 09:00:00 Instrumentation ChangeExploris_Dabney2021-03-29 06:32:222021-03-29 06:32:22
Service by John Polese.
Action: Orbitrap block with CT assembly replaced
Reason: negative mode performance unstable
2021-03-17 12:00:00 Instrumentation ChangeExploris_PSB2021-03-26 09:20:452021-03-26 09:20:45
Prepared and changed fresh solvent2021-03-16 11:00:00 Reagent ChangeExploris_Dabney2021-04-05 07:05:182021-04-05 07:13:57
(1) HCTT cleaned (2) Mass calibration.2021-03-15 00:00:00 Instrumentation ChangeExploris_Dabney2021-03-15 07:12:592021-03-15 07:12:59