The peptide mixture standard consists of 6 peptides that were specifically synthesized to cover a wide range of hydrophobicities (Grand Average Hydropathy scores -0.6-1.9).   Within each peptide sequence, a combination of stable isotope labeled amino acids (¹³C and ¹⁵N) were inserted to create 5 isotopologues.  These 5 peptide isotopologues, based on amino acid analysis, span 4 orders of magnitude in concentration within each distinct sequence.  One can assess numerous fundamental analytical figures of merit as a function of intra-scan dynamic range across the gradient within a single injection.

Below, you may download several Skyline template files for the peptide reference mixture.  The files designated MS1/MS2 filtering will extract only precursors and product ions, respectively.  The largest file will extract both.

Please direct all inquiries to Michael Bereman at msberema@ncsu.edu.