Date of sample prep: 03 October 2021

Samples: Enriched extracellular particles from plasma, lumbar CSF, and ventricular CSF

Sample prep protocol: automated SP3 with Kingfisher

Date of acquisition: 08 October - 13 October 2021

LC system: EvoSep C18, EV-2001

Instrument: Thermo Exploris

Overview: Whole plasma, enriched plasma EEVs, whole CSF, and enriched CSF EEVs were prepped in replicate by Christine. Samples were analyzed as separate batches with their own chromatogram libraries. This was to examine the reproducibility of the sample prep and enrichment protocols. 

Sample preparation protocols & notes