3x3 Experiment

Date of sample prep: 19 March 2021 (first set), 22 March 2021 (second set), 24 March 2021 (third set)

Samples: Pooled ventricular CSF 

Sample prep protocol: automated SP3 with Kingfisher

Instrument: Thermo Exploris

 

Overview: 4 replicates (1 to be considered a batch reference, hence it being it a 3x3) were prepped using the same protocol, on 3 different days. They were analyzed as separate batches with their own chromatogram libraries. This was to examine the reproducibility of the sample prep protocol, and to compare proteome coverage of 2 gradient lengths (44 minutes and 88 minutes) on the EvoSep LC system. 

By clicking through these pages you can find things such as protocol, run logs etc