AbLotComparison_2025-09-11_13-39-42.sky.zip: /TAMADOR/UW Medicine - NIDDK U01 Diabetes Assays/Proinsulin Assay/Proinsulin LC-MSMS Paper/01_Method Development

AbLotComparison_2025-09-11_13-39-42.sky.zip

01_Method Development

Document Summary

AbLotComparison_2025-09-11_13-39-42.sky.zip 1 version

3 molecule lists, 2 peptides, 4 small molecules, 8 precursors, 24 transitions - 16 replicates, 6 calibration curves - Skyline-daily (64-bit) 25.1.1.206 (2ad5a87e4)

Precursor List

Protein Note/Annotations

Peptide Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Peptide Note/Annotations	Missed Cleavages Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Peptide Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Rank Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Precursor Note/Annotations	Label Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Q1 m/z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Transition Count Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	CE Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	DP Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
RGFFYTPKTRREAE		0	1757.9576		RGFFYTPKTRREAE		light	4+	440.4967	1757.9576	3	14.5299	63.2292
RGFFYTPKTRREAE		0	1757.9576		RGFFYTPKTRREAE		heavy	4+	442.9788	1767.8862	3	14.5299	63.2292

Peptide Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Peptide Note/Annotations	Missed Cleavages Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Peptide Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Rank Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Precursor Note/Annotations	Label Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Q1 m/z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Transition Count Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	CE Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	DP Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
GSLQKRGIVE		0	1086.2496		GSLQKRGIVE		light	2+	544.1321	1086.2496	3	19.0669	70.7842
GSLQKRGIVE		0	1086.2496		GSLQKRGIVE		heavy	2+	549.0964	1096.1783	3	19.0669	70.7842

Small Molecule Precursor List

Molecule List Note/Annotations

Molecule Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Ion Formula Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Molecule Note/Annotations	Mass Average Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Mass Monoisotopic Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Precursor Note/Annotations	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Q1 m/z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Mass Average Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Mass Monoisotopic Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Transition Count Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
RGFFYTPKTRREAE.4			1762.0022	1762.0022	RGFFYTPKTRREAE.4		4+	440.5000	1762.0022	1762.0022	3
RGFFYTPKTRREAE Heavy			1771.8822	1771.8822	RGFFYTPKTRREAE Heavy		4+	442.9700	1771.8822	1771.8822	3
GSLQKRGIVE.2			2176.5222	2176.5222	GSLQKRGIVE.2		4+	544.1300	2176.5222	2176.5222	3
GSLQKRGIVE Heavy			2196.4022	2196.4022	GSLQKRGIVE Heavy		4+	549.1000	2196.4022	2196.4022	3