IAA_peptides_2025-09-11_13-36-33.sky.zip: /TAMADOR/UW Medicine - NIDDK U01 Diabetes Assays/Proinsulin Assay/Proinsulin LC-MSMS Paper/01_Method Development

IAA_peptides_2025-09-11_13-36-33.sky.zip

01_Method Development

Document Summary

IAA_peptides_2025-09-11_13-36-33.sky.zip 1 version

4 molecule lists, 3 peptides, 7 small molecules, 12 precursors, 38 transitions - 79 replicates, 10 calibration curves - Skyline-daily (64-bit) 25.1.1.206 (2ad5a87e4)

Precursor List

Protein Note/Annotations

Peptide Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Peptide Note/Annotations	Missed Cleavages Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Peptide Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Rank Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Precursor Note/Annotations	Label Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Q1 m/z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Transition Count Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	CE Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	DP Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
RGFFYTPKTRREAE		4	1757.9576		RGFFYTPKTRREAE		light	4+	440.4967	1757.9576	3	14.5299	63.2292
RGFFYTPKTRREAE		4	1757.9576		RGFFYTPKTRREAE		heavy	4+	442.9788	1767.8862	3	14.5299	63.2292
GSLQKRGIVE		2	1086.2496		GSLQKRGIVE		light	2+	544.1321	1086.2496	3	19.0669	70.7842
GSLQKRGIVE		2	1086.2496		GSLQKRGIVE		heavy	2+	549.0964	1096.1783	3	19.0669	70.7842

Peptide Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Peptide Note/Annotations	Missed Cleavages Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Peptide Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Rank Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Precursor Note/Annotations	Label Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Q1 m/z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Transition Count Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	CE Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	DP Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
RGFFYTPKTRRE		4	1557.7645		RGFFYTPKTRRE		light	4+	390.4484	1557.7645	5	12.7281	59.5807

Small Molecule Precursor List

Molecule List Note/Annotations

Molecule Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Ion Formula Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Molecule Note/Annotations	Mass Average Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Mass Monoisotopic Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Precursor Note/Annotations	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Q1 m/z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Mass Average Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Mass Monoisotopic Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Transition Count Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
RGFFYTPKTRREAE			1755.4509	1755.4509	RGFFYTPKTRREAE		4+	439.8700	1755.4509	1755.4509	3
GSLQKRGIVE			1084.4454	1084.4454	GSLQKRGIVE		2+	543.2300	1084.4454	1084.4454	3
RGFFYTPKTRREAEDLQVGQVE			2623.1709	2623.1709	RGFFYTPKTRREAEDLQVGQVE		4+	656.8000	2623.1709	2623.1709	3
RGFFYTPKTRRE			1557.2909	1557.2909	RGFFYTPKTRRE		4+	390.3300	1557.2909	1557.2909	3

Molecule Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Ion Formula Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Molecule Note/Annotations	Mass Average Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Mass Monoisotopic Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Precursor Note/Annotations	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Q1 m/z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Mass Average Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Mass Monoisotopic Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Transition Count Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
RGFFYTPKTRREAE			1765.0509	1765.0509	RGFFYTPKTRREAE		4+	442.2700	1765.0509	1765.0509	3
GSLQKRGIVE			1094.4054	1094.4054	GSLQKRGIVE		2+	548.2100	1094.4054	1094.4054	3
RGFFYTPKTRREAEDLQVGQVE			2634.7709	2634.7709	RGFFYTPKTRREAEDLQVGQVE		4+	659.7000	2634.7709	2634.7709	3