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  1. Targeted MS Runs
  2. Comparison between multi-format vs single-format_2023-06-09_13-10-17.sky.zip
  3. Peptide Details

Precursor Chromatograms: AISSIGLEC[+57.0]QSVTSR[+10.0]

Multi-IA-LC-MS strategy for leptin, resistin, and adiponectin

Precursor Summary

 
File Comparison between multi-format vs single-format_2023-06-09_13-10-17.sky.zip   
14 MB 
Group RETN
Peptide AISSIGLECQSVTSR
Precursor
AISSIGLECQSVTSR
Label heavy
Charge 2
m/z 809.4106

Chromatograms

 
LRA_Sample1_01 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_pooled_hpH_01), 2023-02-03 01:41:31
AISSIGLECQSVTSR - 809.4106++ (heavy)
y10 - 1146.5447+6.26.46.66.87.00.00.20.40.60.81.01.21.41.61.82.02.2Intensity 10^36.6
Retention Time
LRA_Sample1_02 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_pooled_hpH_02), 2023-02-03 01:57:45
AISSIGLECQSVTSR - 809.4106++ (heavy)
y10 - 1146.5447+6.26.46.66.87.00.00.20.40.60.81.01.21.41.61.82.02.2Intensity 10^36.6
Retention Time
LRA_Sample1_03 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_pooled_hpH_03), 2023-02-03 02:13:59
AISSIGLECQSVTSR - 809.4106++ (heavy)
y10 - 1146.5447+6.26.46.66.87.00.00.20.40.60.81.01.21.41.61.82.0Intensity 10^36.6
Retention Time
LRA_Sample2_01 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_indi_pool_1_01), 2023-02-03 03:18:57
AISSIGLECQSVTSR - 809.4106++ (heavy)
y10 - 1146.5447+6.26.46.66.87.07.20.00.10.20.30.40.50.60.70.80.91.01.11.21.31.41.51.61.7Intensity 10^36.5
Retention Time
LRA_Sample2_02 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_indi_pool_1_02), 2023-02-03 03:35:11
AISSIGLECQSVTSR - 809.4106++ (heavy)
y10 - 1146.5447+6.26.46.66.87.00.00.20.40.60.81.01.21.41.61.82.02.2Intensity 10^36.6
Retention Time
LRA_Sample2_03 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_indi_pool_1_03), 2023-02-03 03:51:25
AISSIGLECQSVTSR - 809.4106++ (heavy)
y10 - 1146.5447+6.26.46.66.87.00.00.10.20.30.40.50.60.70.80.91.01.11.21.31.41.51.61.7Intensity 10^36.5
Retention Time
LRA_Sample3_01 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_indi_pool_2_01), 2023-02-03 04:07:39
AISSIGLECQSVTSR - 809.4106++ (heavy)
y10 - 1146.5447+6.26.46.66.87.00.00.20.40.60.81.01.21.41.61.82.0Intensity 10^36.6
Retention Time
LRA_Sample3_02 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_indi_pool_2_02), 2023-02-03 04:23:53
AISSIGLECQSVTSR - 809.4106++ (heavy)
y10 - 1146.5447+6.26.46.66.87.07.20.00.20.40.60.81.01.21.41.61.82.02.22.4Intensity 10^36.6
Retention Time
LRA_Sample3_03 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_indi_pool_2_03), 2023-02-03 04:40:07
AISSIGLECQSVTSR - 809.4106++ (heavy)
y10 - 1146.5447+6.26.46.66.87.07.20.00.10.20.30.40.50.60.70.80.91.01.11.21.31.41.51.61.7Intensity 10^36.6
Retention Time
L_Sample1_01 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_L_pooled_hpH_01), 2023-02-02 15:16:57
AISSIGLECQSVTSR - 809.4106++ (heavy)
y10 - 1146.5447+6.26.46.66.87.00.00.20.40.60.81.01.21.41.61.82.0Intensity 10^36.5
Retention Time

Summary Charts

 

Peak Areas

AISSIGLECQSVTSR
LRA_Sample1_01LRA_Sample1_02LRA_Sample1_03LRA_Sample2_01LRA_Sample2_02LRA_Sample2_03LRA_Sample3_01LRA_Sample3_02LRA_Sample3_03L_Sample1_01L_Sample1_02L_Sample2_01L_Sample2_02L_Sample2_03L_Sample3_01L_Sample3_02L_Sample3_03R_Sample1_01R_Sample1_02R_Sample1_03R_Sample2_01R_Sample2_02R_Sample2_03R_Sample3_01R_Sample3_02R_Sample3_03A_Sample1_01A_Sample1_02A_Sample1_03A_Sample2_01A_Sample2_02A_Sample2_03A_Sample3_01A_Sample3_02A_Sample3_030.02.04.06.08.010.012.014.0Peak Area 10^3
Replicates

Retention Times

AISSIGLECQSVTSR
LRA_Sample1_01LRA_Sample1_02LRA_Sample1_03LRA_Sample2_01LRA_Sample2_02LRA_Sample2_03LRA_Sample3_01LRA_Sample3_02LRA_Sample3_03L_Sample1_01L_Sample1_02L_Sample2_01L_Sample2_02L_Sample2_03L_Sample3_01L_Sample3_02L_Sample3_03R_Sample1_01R_Sample1_02R_Sample1_03R_Sample2_01R_Sample2_02R_Sample2_03R_Sample3_01R_Sample3_02R_Sample3_03A_Sample1_01A_Sample1_02A_Sample1_03A_Sample2_01A_Sample2_02A_Sample2_03A_Sample3_01A_Sample3_02A_Sample3_036.46.46.56.56.66.66.76.76.86.86.96.9Retention Time
Replicates

AISSIGLEC[+57.0]QSVTSR[+10.0], Charge 2 (heavy)

 
Ions:
a 1+ 2+ 3+
b 1+ 2+ 3+
c 1+ 2+ 3+
x 1+ 2+ 3+
y 1+ 2+ 3+
z 1+ 2+ 3+
[Deselect All]
Neutral Loss:
NH3 (*)
H2O (o)
Immonium ions
Reporter ions
Mass Type:
Mono Avg
Mass Tol:
Peak Assignment:
Most Intense
Nearest Match
Peak Detect
Peak Labels:
Ionm/z
None
Width: 600
Height: 450
AISSIGLECQSVTSR, MH+ 1617.7843, m/z 809.3958
b+#Seq#y+
72.04441A15 
185.12852I141546.7472
272.16053S131433.6631
359.19254S121346.6311
472.27665I111259.5990
529.29806G101146.5150
642.38217L91089.4935
771.42478E8976.4095
931.43399C7847.3669
1059.492510Q6687.3577
1146.524511S5559.2991
1245.592912V4472.2671
1346.640613T3373.1987
1433.672614S2272.1510
 15R1185.1190
[Click] to move table
Static Modifications:
C: 57
Variable Modifications:
R: 10[15]
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1400
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