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  1. Targeted MS Runs
  2. Comparison between multi-format vs single-format_2023-06-09_13-10-17.sky.zip
  3. Peptide Details

Precursor Chromatograms: GDIGETGVPGAEGPR[+10.0]

Multi-IA-LC-MS strategy for leptin, resistin, and adiponectin

Precursor Summary

 
File Comparison between multi-format vs single-format_2023-06-09_13-10-17.sky.zip   
14 MB 
Protein ADIP
Peptide GDIGETGVPGAEGPR
Precursor
GDIGETGVPGAEGPR
Label heavy
Charge 2
m/z 711.3484

Chromatograms

 
LRA_Sample1_01 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_pooled_hpH_01), 2023-02-03 01:41:31
GDIGETGVPGAEGPR - 711.3484++ (heavy)
y7 - 693.3554+5.25.45.65.86.06.20.02.04.06.08.010.012.014.016.018.0Intensity 10^35.6
Retention Time
LRA_Sample1_02 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_pooled_hpH_02), 2023-02-03 01:57:45
GDIGETGVPGAEGPR - 711.3484++ (heavy)
y7 - 693.3554+5.25.45.65.86.06.20.02.04.06.08.010.012.014.016.018.020.0Intensity 10^35.6
Retention Time
LRA_Sample1_03 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_pooled_hpH_03), 2023-02-03 02:13:59
GDIGETGVPGAEGPR - 711.3484++ (heavy)
y7 - 693.3554+5.25.45.65.86.06.20.02.04.06.08.010.012.014.016.018.020.022.0Intensity 10^35.6
Retention Time
LRA_Sample2_01 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_indi_pool_1_01), 2023-02-03 03:18:57
GDIGETGVPGAEGPR - 711.3484++ (heavy)
y7 - 693.3554+5.25.45.65.86.06.20.02.04.06.08.010.012.014.016.018.020.022.0Intensity 10^35.7
Retention Time
LRA_Sample2_02 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_indi_pool_1_02), 2023-02-03 03:35:11
GDIGETGVPGAEGPR - 711.3484++ (heavy)
y7 - 693.3554+5.25.45.65.86.00.02.04.06.08.010.012.014.016.018.0Intensity 10^35.6
Retention Time
LRA_Sample2_03 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_indi_pool_1_03), 2023-02-03 03:51:25
GDIGETGVPGAEGPR - 711.3484++ (heavy)
y7 - 693.3554+5.25.45.65.86.06.20.02.04.06.08.010.012.014.016.018.020.0Intensity 10^35.6
Retention Time
LRA_Sample3_01 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_indi_pool_2_01), 2023-02-03 04:07:39
GDIGETGVPGAEGPR - 711.3484++ (heavy)
y7 - 693.3554+5.25.45.65.86.06.20.02.04.06.08.010.012.014.016.018.020.022.0Intensity 10^35.6
Retention Time
LRA_Sample3_02 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_indi_pool_2_02), 2023-02-03 04:23:53
GDIGETGVPGAEGPR - 711.3484++ (heavy)
y7 - 693.3554+5.25.45.65.86.00.02.04.06.08.010.012.014.016.018.020.022.0Intensity 10^35.7
Retention Time
LRA_Sample3_03 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_LRA_indi_pool_2_03), 2023-02-03 04:40:07
GDIGETGVPGAEGPR - 711.3484++ (heavy)
y7 - 693.3554+5.25.45.65.86.06.20.02.04.06.08.010.012.014.016.018.020.022.0Intensity 10^35.7
Retention Time
L_Sample1_01 (02022023_TC07_lowpH_15min_0p3de_3%_4to50%_R0p7_50uL_IP_L_pooled_hpH_01), 2023-02-02 15:16:57
GDIGETGVPGAEGPR - 711.3484++ (heavy)
y7 - 693.3554+5.05.25.45.65.86.06.20.01.02.03.04.05.06.07.08.09.010.011.012.013.014.015.016.0Intensity 10^35.6
Retention Time

Summary Charts

 

Peak Areas

GDIGETGVPGAEGPR
LRA_Sample1_01LRA_Sample1_02LRA_Sample1_03LRA_Sample2_01LRA_Sample2_02LRA_Sample2_03LRA_Sample3_01LRA_Sample3_02LRA_Sample3_03L_Sample1_01L_Sample1_02L_Sample2_01L_Sample2_02L_Sample2_03L_Sample3_01L_Sample3_02L_Sample3_03R_Sample1_01R_Sample1_02R_Sample1_03R_Sample2_01R_Sample2_02R_Sample2_03R_Sample3_01R_Sample3_02R_Sample3_03A_Sample1_01A_Sample1_02A_Sample1_03A_Sample2_01A_Sample2_02A_Sample2_03A_Sample3_01A_Sample3_02A_Sample3_03020406080100120140Peak Area 10^3
Replicates

Retention Times

GDIGETGVPGAEGPR
LRA_Sample1_01LRA_Sample1_02LRA_Sample1_03LRA_Sample2_01LRA_Sample2_02LRA_Sample2_03LRA_Sample3_01LRA_Sample3_02LRA_Sample3_03L_Sample1_01L_Sample1_02L_Sample2_01L_Sample2_02L_Sample2_03L_Sample3_01L_Sample3_02L_Sample3_03R_Sample1_01R_Sample1_02R_Sample1_03R_Sample2_01R_Sample2_02R_Sample2_03R_Sample3_01R_Sample3_02R_Sample3_03A_Sample1_01A_Sample1_02A_Sample1_03A_Sample2_01A_Sample2_02A_Sample2_03A_Sample3_01A_Sample3_02A_Sample3_035.45.55.55.65.65.75.75.85.85.95.96.0Retention Time
Replicates

GDIGETGVPGAEGPR[+10.0], Charge 2 (heavy)

 
Ions:
a 1+ 2+ 3+
b 1+ 2+ 3+
c 1+ 2+ 3+
x 1+ 2+ 3+
y 1+ 2+ 3+
z 1+ 2+ 3+
[Deselect All]
Neutral Loss:
NH3 (*)
H2O (o)
Immonium ions
Reporter ions
Mass Type:
Mono Avg
Mass Tol:
Peak Assignment:
Most Intense
Nearest Match
Peak Detect
Peak Labels:
Ionm/z
None
Width: 600
Height: 450
GDIGETGVPGAEGPR, MH+ 1421.6812, m/z 711.3442
b+#Seq#y+
58.02871G15 
173.05572D141364.6597
286.13973I131249.6328
343.16124G121136.5487
472.20385E111079.5273
573.25156T10950.4847
630.27297G9849.4370
729.34148V8792.4155
826.39419P7693.3471
883.415610G6596.2944
954.452711A5539.2729
1083.495312E4468.2358
1140.516813G3339.1932
1237.569514P2282.1717
 15R1185.1190
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Variable Modifications:
R: 10[15]
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