Figure 4C Impact of EF-G (P610L) on the proteome integrity in the inner membrane proteins

 

Figure 4C: Impact of EF-G (P610L) on the proteome integrity in the inner membrane proteins.

Error frequencies of 109 misreading events in 28 proteins were determined by label-free PRM.

 

Figure S7D: Impact of EF-G (P610L) on the proteome integrity in the inner membrane proteins in the untreated and Apr-treated (16µM, 60 min) cells. 

Error frequencies of 109 misreading events in 28 proteins were determined by label free parallel reaction monitoring. Error frequencies are the means ± SD of three biological replicates of the medians of 1-12 misreading events, as indicated above the bars.


Overview of Skyline files:

Skyline file Proteins
Figure 4C_Error load membrane proteins_B01       MlaD
Figure 4C_Error load membrane proteins_B02 PlsB
Figure 4C_Error load membrane proteins_B03 FtsH
Figure 4C_Error load membrane proteins_B04 PpiD
Figure 4C_Error load membrane proteins_B05 RodZ
Figure 4C_Error load membrane proteins_B06 HflK
Figure 4C_Error load membrane proteins_B07 DacA, TreB
Figure 4C_Error load membrane proteins_B08 HflC
Figure 4C_Error load membrane proteins_B09 GatC, WzzB
Figure 4C_Error load membrane proteins_B10 MetQ, Psd
Figure 4C_Error load membrane proteins_B11 AtpF, YajG, YgiM, YqjD
Figure 4C_Error load membrane proteins_B12 DsbB, FxsA
Figure 4C_Error load membrane proteins_B13 FrdC, NuoA, PspC, SdhC, SecE, TatA, YhcB
Figure 4C_Error load membrane proteins_B14 SdhD, YajC

Clustergrammer Heatmap
 
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Figure 4C_Error load membrane proteins_B14_2024-07-05_15-03-44.sky.zip2025-04-19 21:44:4316161816312
Figure 4C_Error load membrane proteins_B13_2024-07-05_15-01-09.sky.zip2025-04-19 21:44:4357586760612
Figure 4C_Error load membrane proteins_B12_2024-07-05_14-56-47.sky.zip2025-04-19 21:44:4331313834212
Figure 4C_Error load membrane proteins_B11_2024-07-05_14-54-31.sky.zip2025-04-19 21:44:4352525953212
Figure 4C_Error load membrane proteins_B10_2024-07-05_14-49-58.sky.zip2025-04-19 21:44:4315152219812
Figure 4C_Error load membrane proteins_B09_2024-07-05_14-48-32.sky.zip2025-04-19 21:44:4315151816312
Figure 4C_Error load membrane proteins_B08_2024-07-05_14-46-18.sky.zip2025-04-19 21:44:4318192118912
Figure 4C_Error load membrane proteins_B07_2024-07-05_14-44-52.sky.zip2025-04-19 21:44:4310101210812
Figure 4C_Error load membrane proteins_B06_2024-07-05_14-39-01.sky.zip2025-04-19 21:44:4312121210812
Figure 4C_Error load membrane proteins_B05_2024-07-05_14-37-44.sky.zip2025-04-19 21:44:4312121211012
Figure 4C_Error load membrane proteins_B04_2024-07-05_14-36-13.sky.zip2025-04-19 21:44:4328283127912
Figure 4C_Error load membrane proteins_B03_2024-07-05_13-52-50.sky.zip2025-04-19 21:44:4334344036212
Figure 4C_Error load membrane proteins_B02_2024-07-05_13-47-32.sky.zip2025-04-19 21:44:438898112
Figure 4C_Error load membrane proteins_B01_2024-07-05_13-27-31.sky.zip2025-04-19 21:44:4399109012