Table of Contents |
guest 2025-09-16 |
LC-IMS-MS/MS sample description
Cross-linking mass spectrometry experimental design
Scheme of photoaffinity labeling. NPY containing photo-leucines at positions 17, 24, and 30 was crosslinked to bicelle-reconstituted Y2R preparations by UV-A light. The crosslinked complex is enzymatically digested, peptides are analyzed by LC-IMS-MS/MS using the MeroX software.
LC-IMS-MS/MS sample description
| Replicate | Sample ID | Group | Acquisition Order | Condition | Chromatography Condition | MS Mode |
| timsTOF_090822_pMet_Y2R_no_pNPY | S-Trap Control (low CE; 20 µg Y2R-bicelle) | S-Trap Control (low CE; 20 µg Y2R-bicelle) | 3389 | Y2R-Bicelle Control | µPAC (90 min) | DDA-PASEF |
| timsTOF_090822_pMet_Y2R-pNPY_XL_1 | S-Trap XL (low CE; 20 µg Y2R-bicelle) 1 | S-Trap XL (low CE; 20 µg Y2R-bicelle) | 3391 | Y2R-Bicelle |
µPAC (90 min) |
DDA-PASEF |
| timsTOF_090822_pMet_Y2R-pNPY_XL_2 | S-Trap XL (low CE; 20 µg Y2R-bicelle) 2 | S-Trap XL (low CE; 20 µg Y2R-bicelle) | 3393 | Y2R-Bicelle | µPAC (90 min) | DDA-PASEF |
| timsTOF_090822_pMet_Y2R-pNPY_XL_3 | S-Trap XL (low CE; 20 µg Y2R-bicelle) 3 | S-Trap XL (low CE; 20 µg Y2R-bicelle) | 3395 | Y2R-Bicelle | µPAC (90 min) | DDA-PASEF |
| timsTOF_090922_pMet_Y2R-pNPY_XL_4 | S-Trap XL (high CE; Y2R-bicelle) 1 | S-Trap XL (high CE; Y2R-bicelle) | 3521 | Y2R-Bicelle | µPAC (90 min) | DDA-PASEF |
| timsTOF_2010922_N-term-Y2R_1-45-no_pNPY | S-Trap Control (low CE; 20 µg N-term) 1 | S-Trap Control (low CE; 20 µg N-term) | 3628 | NT-Y2R Control | Self-Packed (3-50; 90 min) | DDA-PASEF |
| timsTOF_2010922_N-term-Y2R_1-45-pNPY_XL_1 | S-Trap XL (20 µg NT-Y2R) 1 | S-Trap XL (20 µg NT-Y2R) | 3631 | NT-Y2R | Self-Packed (3-50; 90 min) | DDA-PASEF |
| timsTOF_2010922_N-term-Y2R_1-45-pNPY_XL_2 | S-Trap XL (20 µg NT-Y2R) 2 | S-Trap XL (20 µg NT-Y2R) | 3632 | NT-Y2R | Self-Packed (3-50; 90 min) | DDA-PASEF |
| timsTOF_2010922_N-term-Y2R_1-45-pNPY_XL_3 | S-Trap XL (20 µg NT-Y2R) 3 | S-Trap XL (20 µg NT-Y2R) | 3633 | NT-Y2R | Self-Packed (3-50; 90 min) | DDA-PASEF |
| timsTOF_20221026_N-term-Y2R_1-45-pNPY_XL_1_low-CE | S-Trap XL (low CE; 20 µg NT-Y2R) 1 | S-Trap XL (low CE; 20 µg NT-Y2R) | 3655 | NT-Y2R | Self-Packed (3-50; 90 min) | DDA-PASEF |
| timsTOF_20221026_N-term-Y2R_1-45-pNPY_XL_2_low-CE | S-Trap XL (low CE; 20 µg NT-Y2R) 2 | S-Trap XL (low CE; 20 µg NT-Y2R) | 3656 | NT-Y2R | Self-Packed (3-50; 90 min) | DDA-PASEF |
| timsTOF_20221026_N-term-Y2R_1-45-pNPY_XL_3_low-CE | S-Trap XL (low CE; 20 µg NT-Y2R) 3 | S-Trap XL (low CE; 20 µg NT-Y2R) | 3657 | NT-Y2R | Self-Packed (3-50; 90 min) | DDA-PASEF |
| timsTOF_20230129_N-term-Y2R_1-45-pNPY_XL_DIA_1 | S-Trap XL (DIA-PASEF; 20 µg NT-Y2R) 1 | S-Trap XL (DIA-PASEF; 20 µg NT-Y2R) | 3920 | NT-Y2R | Self-Packed (3-50; 90 min) | DIA-PASEF |
| timsTOF_20230129_Y2R-pNPY_DIA_01 | S-Trap XL (DIA-PASEF; 20 µg Y2R-bicelle) 1 | S-Trap XL (DIA-PASEF; 20 µg Y2R-bicelle) | 3921 | Y2R-Bicelle | Self-Packed (3-50; 90 min) | DIA-PASEF |
| timsTOF_20230302_Y2R-pNPY_XL | S-Trap XL (low CE; 20 µg Y2R-bicelle) long gradient 1 | S-Trap XL (low CE; 20 µg Y2R-bicelle) long gradient | 4101 | Y2R-Bicelle | Self-Packed (3-35; 90 min) | DDA-PASEF |
| timsTOF_20230302_N-term-Y2R_1-45-pNPY_XL | S-Trap XL (low CE; 20 µg NT-Y2R) long gradient 1 | S-Trap XL (low CE; 20 µg NT-Y2R) long gradient | 4102 | NT-Y2R | Self-Packed (3-35; 90 min) | DDA-PASEF |
| timsTOF_20241215_Y2R-pNPY_JNJ_1 | S-Trap XL (low CE; 20 µg Y2R-bicelle) long gradient 1 | 8683 | Y2R-Bicelle | Self-Packed (3-35; 90 min) | DDA-PASEF | |
| timsTOF_20241215_Y2R-pNPY_JNJ_2 | S-Trap XL (low CE; 20 µg Y2R-bicelle) long gradient 1 | 8684 | Y2R-Bicelle | Self-Packed (3-35; 90 min) | DDA-PASEF | |
| timsTOF_20241215_Y2R-pNPY_JNJ_3 | S-Trap XL (low CE; 20 µg Y2R-bicelle) long gradient 1 | 8685 | Y2R-Bicelle | Self-Packed (3-35; 90 min) | DDA-PASEF | |
| timsTOF_20241215_Y2R-pNPY_NPY_1 | S-Trap XL (low CE; 20 µg Y2R-bicelle) long gradient 1 | 8687 | Y2R-Bicelle | Self-Packed (3-35; 90 min) | DDA-PASEF | |
| timsTOF_20241215_Y2R-pNPY_NPY_2 | S-Trap XL (low CE; 20 µg Y2R-bicelle) long gradient 1 | 8688 | Y2R-Bicelle | Self-Packed (3-35; 90 min) | DDA-PASEF | |
| timsTOF_20241215_Y2R-pNPY_NPY_3 | S-Trap XL (low CE; 20 µg Y2R-bicelle) long gradient 1 | 8689 | Y2R-Bicelle | Self-Packed (3-35; 90 min) | DDA-PASEF | |
| timsTOF_20241215_Y2R-pNPY_no_UV | S-Trap XL (low CE; 20 µg Y2R-bicelle) long gradient 1 | 8691 | Y2R-Bicelle | Self-Packed (3-35; 90 min) | DDA-PASEF |