| Table SI 3: Analytical Performance Metrics of developed SRM Assays, as measured for Quality Control and in Bound and Non-bound Fractions (see also Figure SI 1). | ||||||||||||||||
| Regression parameters are derived from the response curve of synthetic peptides in the solvent matrix, using a 1/x weighting for regression analysis. 'Fraction Type' indicates the format of the measurement. Intra-assay (within day) and Inter-assay (between day) values represent the average precision from replicate measurements (n=5). 'High', 'Medium', and 'Low' refer to synthetic peptide mix samples prepared at three concentration levels. All reported signals are above the Lower Limit of Quantification (LLOQ) in replicate measurements. 'Fragment ion / transition' indicates the specific ion applied for quantification. The table includes data on linearity, sensitivity, and reproducibility across different concentration levels and measurement conditions, providing a comprehensive overview of the assay's analytical performance. | ||||||||||||||||
| intra-assay precision | inter-assay precision | |||||||||||||||
| zebrafish gene corresponding to target protein |
peptide modified sequence [sequence range] | fraction | slope | intercept | correlation coefficient | LOD [fmol/µL] | LOQ [fmol/µL] | linear range (log 10) | fragment ion / transition | low | medium | high | low | medium | high | |
| actba | K.AGFAGDDAPR.A [19, 28] | non-bound | 4.016E+03 | -8.275E+02 | 0.9908 | 0.2074 | 0.6913 | 2.21 | G [y6] - 640.2924+ | 4.75 | 3.14 | 2.78 | 5.72 | 3.80 | 3.08 | |
| R.GYSFTTTAER.E [197, 206] | non-bound | 1.331E+03 | -2.635E+02 | 0.993 | 0.2167 | 0.7223 | 2.19 | T [y6] - 688.3500+ | 7.29 | 2.01 | 1.57 | 8.19 | 3.72 | 2.78 | ||
| akt1 | K.EGITDGATMK.T [292, 301] | non-bound | 5.114E+00 | -6.772E-01 | 0.9846 | 0.6901 | 2.3003 | 2.19 | T [y7] - 723.3342+ | n.d. | n.d. | 6.72 | n.d. | n.d. | 7.14 | |
| K.FFTGIVWQDVYEK.K [401, 413] | non-bound | 3.005E+02 | -2.804E+02 | 0.9639 | 1.2539 | 4.1797 | 1.42 | V [y8] - 1074.5346+ | 19.77 | 6.68 | 2.82 | 37.14 | 18.67 | 4.21 | ||
| R.RPHFPQFS[+80]YSASGTA.- [460, 474] | bound | 5.729E+01 | -1.662E+03 | 0.9086 | 29.3962 | 97.9873 | 0.05 | precursor -98 - 822.9028++ | n.d. | n.d. | 4.56 | n.d. | n.d. | 5.47 | ||
| R.RPHFPQFSYSASGTA.- [460, 474] | non-bound | 8.266E+01 | -5.424E+02 | 0.8823 | 10.8451 | 36.1503 | 0.99 | F [b4] - 538.2885+ | 22.99 | 11.28 | 8.18 | 25.43 | 19.79 | 15.01 | ||
| akt2 | K.EGITNEATMK.T [297, 306] | non-bound | 9.354E+02 | -1.201E+02 | 0.9919 | 0.1659 | 0.5530 | 2.81 | T [y7] - 802.3855+ | 11.24 | 3.10 | 1.88 | 12.78 | 4.92 | 2.56 | |
| R.THFSQFS[+80]YSASVR.E [466, 478] | bound | 1.186E+03 | 2.859E+02 | 0.9687 | 0.054 | 0.1800 | 2.79 | precursor -98 - 503.5767+++ | 22.39 | 12.85 | 5.74 | 27.41 | 14.52 | 11.56 | ||
| R.THFSQFSYSASVR.E [466, 478] | non-bound | 7.579E+02 | 1.172E+03 | 0.9951 | 0.5552 | 1.8507 | 2.29 | S [y5] - 519.2885+ | 13.40 | 4.08 | 1.75 | 14.67 | 4.88 | 2.98 | ||
| akt3 | K.DEVAHTLTESR.V [188, 198] | QC_both | 1.200E+02 | -1.140E+01 | 0.9681 | 1.1884 | 3.9613 | 1.85 | T [y6] - 716.3813+ | 9.53 | 6.96 | 12.20 | 12.16 | |||
| K.EGITDAATMK.T [295, 304] | non-bound | 1.653E+01 | 2.983E+00 | 0.9839 | 0.0449 | 0.1497 | 3.38 | T [y7] - 737.3498+ | 12.41 | 3.98 | 13.16 | 5.77 | ||||
| akt1/2/3 | K.TFC[+57]GTPEYLAPEVLEDNDYGR.A [302, 322] | non-bound | 2.648E+03 | -5.800E+01 | 0.9774 | 0.2795 | 0.9317 | 2.08 | P [y11] - 658.8033++ | 20.48 | 6.17 | 4.19 | 59.33 | 7.90 | 6.12 | |
| K.T[+80]FC[+57]GTPEYLAPEVLEDNDYGR.A [302, 322] | bound | 9.142E+02 | 1.993E+02 | 0.9707 | 0.5633 | 1.8777 | 1.77 | P [y11] - 653.7991++ | 14.04 | 9.41 | 6.26 | 40.01 | 19.54 | 8.10 | ||
| akt1s1/PRAS40 | K.FHPSGIEVEGQR.N [52, 63] | non-bound | 1.117E+03 | -3.325E+03 | 0.9832 | 2.9865 | 9.9550 | 1.55 | P [y10] - 1071.5429+ | 8.51 | 2.61 | 2.51 | 12.76 | 4.23 | 4.57 | |
| R.S[+80]LPVSVPVWGYR.N [284, 295] | bound | 1.371E+03 | 5.108E+02 | 0.9744 | 0.304 | 1.0133 | 2.04 | precursor -98 - 676.3735++ | 10.87 | 6.99 | 6.51 | 22.89 | 8.63 | 11.71 | ||
| R.SLPVSVPVWGYR.N [284, 295] | non-bound | 7.970E+03 | -9.932E+03 | 0.9669 | 1.2697 | 4.2323 | 1.93 | P [y10] - 580.3166++ | 7.64 | 6.11 | 7.14 | 12.87 | 10.33 | 15.14 | ||
| R.VGC[+57]ADLDHIAASMK.A [310, 323] | non-bound | 6.152E+01 | -5.805E+01 | 0.9907 | 1.2725 | 4.2417 | 1.93 | G [y13] - 694.8185++ | 7.50 | 5.18 | 12.39 | 10.41 | ||||
| R.LNT[+80]GDFSLK.H [345, 353] | bound | 1.712E+03 | -1.051E+02 | 0.9913 | 0.1313 | 0.4377 | 2.40 | precursor -98 - 492.7656++ | 12.04 | 4.53 | 4.51 | 14.63 | 4.97 | 8.68 | ||
| R.LNTGDFSLK.H [345, 353] | non-bound | 4.684E+03 | -9.952E+02 | 0.9848 | 0.2196 | 0.7320 | 2.69 | T [y7] - 767.3934+ | 5.45 | 3.22 | 3.77 | 6.60 | 3.50 | 4.37 | ||
| eif4ebp1 | K.FLLDC[+57]RSS[+80]PLAR.T [55, 66] (missed 1) | QC_both | 4.744E+02 | -2.669E+02 | 0.9709 | 0.7786 | 2.5953 | 1.63 | precursor -98 - 713.8786++ | 19.84 | 5.59 | 3.73 | 22.69 | 7.80 | 6.57 | |
| R.SS[+80]PLAR.T [61, 66] | bound | 4.75E+03 | 5.79E+01 | 0.9104 | 0.0025 | 0.0083 | 4.12 | precursor -98 - 311.6810++ | 12.14 | 10.39 | 11.92 | 17.73 | 20.44 | 25.51 | ||
| K.AYPKPTVNNNSISPPVDK.S [91, 108] | non-bound | 2.03E+03 | -2.93E+03 | 0.9905 | 1.4533 | 4.8443 | 1.87 | S [y6] - 642.3457+ | 5.34 | 3.39 | 3.45 | 6.47 | 4.21 | 4.87 | ||
| K.STGEDAQFEMDI.- [109, 120] | non-bound | 2.93E+01 | -1.00E+01 | 0.9643 | 1.9306 | 6.4353 | 1.74 | A [b6] - 561.2151+ | 19.94 | 9.28 | 21.79 | 17.06 | ||||
| Erk2 | R.VADPDHDHTGFLTEYVATR.W [182, 200] | non-bound | 3.78E+02 | -3.04E+03 | 0.9636 | 8.1556 | 27.1853 | 1.12 | P [y16] - 929.9396++ | 9.38 | 5.29 | 17.05 | 9.90 | |||
| R.NYLLSLPLR.S [271, 279] | non-bound | 1.29E+04 | -1.10E+04 | 0.9947 | 0.8809 | 2.9363 | 2.09 | L [y6] - 698.4559+ | 2.62 | 1.70 | 2.23 | 8.44 | 2.77 | 3.19 | ||
| fkbp8 | R.GNVHY[+80]QR.A [221, 227] | bound | 1.43E+02 | -5.68E+01 | 0.9747 | 0.4378 | 1.4593 | 1.88 | H [y4] - 693.2744+ | 7.93 | 4.61 | 4.11 | 11.79 | 9.84 | 9.46 | |
| K.VLALQGEFAEAIK.T [308, 320] | non-bound | 1.18E+03 | 1.10E+04 | 0.9576 | 2.0995 | 6.9983 | 1.71 | G [y8] - 864.4462+ | 12.45 | 4.11 | 2.03 | 19.42 | 19.12 | 4.68 | ||
| K.GAEQAMY[+80]K.K [351, 358] | bound | 4.79E+02 | -7.18E+01 | 0.9926 | 0.1904 | 0.6347 | 2.24 | A [y4] - 600.2343+ | 9.08 | 3.49 | 2.30 | 10.53 | 5.17 | 4.28 | ||
| gapdh | K.VGINGFGR.I [4, 11] | QC_both | 2.51E+03 | 1.46E+01 | 0.9807 | 0.0042 | 0.0139 | 3.90 | N [y5] - 560.2815+ | 6.18 | 3.46 | 3.50 | 7.31 | 3.59 | 3.84 | |
| hif1ab | K.LPLSLSPLTPSDSIPALTK.L [453, 471] | non-bound | 3.63E+03 | -3.83E+03 | 0.9815 | 1.0838 | 3.6127 | 2.00 | P [y10] - 514.7848++ | 6.10 | 4.05 | 11.42 | 6.44 | |||
| K.LPLSLS[+80]PLTPSDSIPALTK.L [453, 471] | bound | 2.12E+02 | -9.80E+01 | 0.9864 | 0.5609 | 1.8695 | 1.77 | P [y5] - 537.3486+ | 15.94 | 2.73 | 41.14 | 4.31 | ||||
| K.TILILPSDVASR.L [720, 731] | non-bound | 1.10E+04 | -9.70E+03 | 0.9949 | 0.8941 | 2.9802 | 2.08 | P [y7] - 731.3682+ | 3.44 | 2.46 | 2.57 | 6.90 | 3.36 | 3.19 | ||
| lamtor1 | K.IAAYAY[+80]SAISQIK.V [134, 146] | QC_both | 1.72E+02 | -4.77E+01 | 0.9841 | 0.8250 | 2.7501 | 1.61 | A [y9] - 1068.5217+ | 17.14 | 5.49 | 1.82 | 22.88 | 10.21 | 5.44 | |
| lamtor2 | K.AEALVQYLEEPLTQVAAS[+80].- [108, 125] | bound | 6.49E+00 | -1.72E+02 | 0.8361 | 29.0593 | 96.8643 | 0.06 | P [y8] - 866.4019+ | 14.45 | 18.31 | |||||
| lamtor5 | R.GSLSDEHGGVVSVLAK.Q [35, 50] | non-bound | 1.34E+04 | -5.11E+03 | 0.9936 | 0.7139 | 2.3797 | 2.18 | S [y13] - 649.3410++ | 4.47 | 2.33 | 1.43 | 6.75 | 4.18 | 3.25 | |
| R.GSLS[+80]DEHGGVVSVLAK.Q [35, 50] | bound | 5.96E+02 | 1.13E+02 | 0.9918 | 0.2917 | 0.9722 | 2.06 | precursor -98 - 515.6101+++ | 7.66 | 1.88 | 8.38 | 2.29 | ||||
| R.HGTITLAVHK.M [79, 88] | non-bound | 1.86E+02 | -8.92E+02 | 0.9602 | 4.9752 | 16.5840 | 1.33 | V [b8] - 793.4567+ | 10.99 | 6.10 | 17.43 | 12.25 | ||||
| lpin1a | R.SDTLGHILPTLGK.D [693, 705] | non-bound | 3.35E+02 | -7.74E+02 | 0.9763 | 2.4286 | 8.0953 | 1.64 | T [y11] - 575.3531++ | 11.61 | 3.24 | 2.45 | 15.31 | 5.77 | 4.32 | |
| K.NLFYPNAEPFYAAFGNR.A [792, 808] | non-bound | 5.99E+02 | -1.05E+03 | 0.9837 | 1.9015 | 6.3383 | 1.75 | P [y9] - 521.7589++ | 19.30 | 6.32 | 5.95 | 64.27 | 9.61 | 9.51 | ||
| K.NLFYPNAEPFY[+80]AAFGNR.A [792, 808] | bound | 2.33E+02 | 5.34E+00 | 0.9585 | 0.6363 | 2.1210 | 1.72 | P [y9] - 566.7462++ | 15.70 | 6.11 | 3.84 | 26.41 | 22.40 | 5.40 | ||
| lpin1 isoform X1 | R.HLGSDGVY[+80]LDDIK.D [375, 387] | bound | 2.44E+02 | 1.97E+00 | 0.9761 | 0.7062 | 2.3540 | 1.67 | G [b6] - 567.2522+ | 6.46 | 8.53 | |||||
| K.DLEPEVAALYFPK.S [388, 400] | non-bound | 7.82E+03 | -1.03E+04 | 0.9739 | 1.3530 | 4.5100 | 1.90 | P [y10] - 567.8133++ | 6.55 | 4.22 | 4.94 | 18.95 | 5.38 | 7.83 | ||
| K.DLEPEVAALY[+80]FPK.S [388, 400] | bound | 3.00E+02 | -5.01E+01 | 0.9734 | 0.4762 | 1.5873 | 1.85 | P [y10] - 1222.5999+ | 13.19 | 4.84 | 4.72 | 35.39 | 12.42 | 6.56 | ||
| R.NLFLPNTEPFY[+80]AAFGNR.D [751, 767] | bound | 4.59E+02 | -1.99E+01 | 0.9494 | 0.6749 | 2.2497 | 1.69 | P [y9] - 566.7462++ | 14.27 | 10.34 | 4.06 | 34.64 | 31.94 | 5.10 | ||
| R.LGEVVDHVFPLLK.R [808, 820] | non-bound | 1.21E+04 | -1.52E+04 | 0.9953 | 1.3851 | 4.6170 | 1.89 | G [y12] - 676.8823++ | 6.55 | 4.38 | 2.99 | 27.32 | 9.66 | 8.37 | ||
| mlst8 | R.TVQHQDSQVNSLEVTPDR.S [37, 54] | non-bound | 1.13E+03 | -9.99E+02 | 0.9961 | 1.0138 | 3.3793 | 2.02 | E [y6] - 716.3573+ | 3.48 | 2.84 | 2.08 | 6.02 | 3.69 | 2.46 | |
| R.TVQHQDS[+80]QVNSLEVTPDR.S [37, 54] | bound | 2.42E+02 | -2.05E+01 | 0.9956 | 0.2892 | 0.9639 | 2.06 | D [y13 -98] - 726.3537++ | 19.82 | 6.81 | 2.23 | 21.58 | 7.81 | 2.73 | ||
| R.SMIAAAGYQHIR.M [55, 66] | non-bound | 1.09E+03 | -8.62E+02 | 0.9843 | 1.0807 | 3.6023 | 2.00 | A [y9] - 493.7620++ | 7.90 | 5.45 | 4.79 | 9.40 | 5.76 | 6.27 | ||
| mTOR | R.VLGLLGALDPYK.H [870, 881] | non-bound | 2.72E+03 | -1.07E+03 | 0.9932 | 0.4294 | 1.4312 | 1.89 | G [y10] - 1054.6023+ | 15.37 | 4.05 | 2.58 | 62.76 | 9.25 | 5.79 | |
| K.LHVS[+80]TTALQK.A [1241, 1250] | bound | 1.62E+03 | -5.01E+02 | 0.9965 | 0.3604 | 1.2013 | 1.97 | precursor -98 - 544.3211++ | 7.16 | 2.08 | 3.13 | 8.04 | 2.44 | 3.74 | ||
| K.LHVSTTALQK.A [1241, 1250] | non-bound | 3.04E+03 | -4.56E+03 | 0.9949 | 1.5162 | 5.0540 | 1.85 | H [b2] - 251.1503+ | 5.36 | 3.53 | 2.36 | 8.18 | 6.44 | 4.98 | ||
| K.GASPLILESLIS[+80]INNK.L [1390, 1405] | bound | 3.51E+01 | 7.16E+00 | 0.9007 | 0.8277 | 2.7591 | 1.60 | precursor -98 - 829.9820++ | 17.25 | 19.12 | ||||||
| K.GASPLILESLISINNK.L [1390, 1405] | non-bound | 4.78E+02 | 1.76E+02 | 0.9421 | 1.2748 | 4.2493 | 1.92 | P [y13] - 727.4349++ | 8.79 | 16.14 | 5.15 | 26.66 | 48.58 | 7.24 | ||
| pak1 | R.SVIEPLPPPLSTK.D [203, 215] | non-bound | 4.66E+03 | -4.56E+03 | 0.9798 | 0.9838 | 3.2793 | 2.04 | P [y9] - 949.5717+ | 4.34 | 2.99 | 3.50 | 6.02 | 3.72 | 4.65 | |
| K.LTDFGFC[+57]AQITPEQSK.R [437, 452] | non-bound | 6.48E+02 | 1.09E+03 | 0.9848 | 0.2685 | 0.8949 | 2.60 | P [y5] - 588.2988+ | 10.26 | 5.00 | 2.89 | 17.88 | 12.83 | 5.06 | ||
| PPAR[g] | K.LEPES[+80]PPQFAENSVSFSK.T [124, 141] | bound | 4.30E+01 | 1.55E+02 | 0.9662 | 3.5607 | 11.8690 | 0.97 | precursor -98 - 661.6577+++ | 6.88 | 8.81 | |||||
| prkaa1/AMPK1 | R.HTLDELNPQK.S [381, 390] | non-bound | 2.47E+02 | -5.51E+02 | 0.9882 | 2.2918 | 7.6393 | 1.67 | L [y8] - 956.5047+ | 18.08 | 4.57 | 2.78 | 19.68 | 6.46 | 5.99 | |
| K.MSLQLYQVDSR.T [452, 462] | non-bound | 1.50E+03 | -1.78E+03 | 0.9941 | 1.3386 | 4.4620 | 1.90 | Y [y6] - 767.3682+ | 3.60 | 2.49 | 1.54 | 8.77 | 4.03 | 3.52 | ||
| K.MSLQLYQVDS[+80]R.T [452, 462] | bound | 1.42E+02 | 8.77E+00 | 0.9555 | 0.2343 | 0.7809 | 2.15 | precursor -98 - 666.3362++ | 14.10 | 19.29 | ||||||
| K.SGTATPHR.S [480, 487] | non-bound | 4.36E+02 | -5.52E+02 | 0.9827 | 1.2984 | 4.3280 | 1.92 | P [y3] - 409.2306+ | 14.43 | 2.73 | 2.66 | 16.06 | 3.79 | 3.65 | ||
| K.SGTAT[+80]PHR.S [480, 487] | bound | 2.17E+02 | -5.35E+01 | 0.9774 | 0.3128 | 1.0427 | 2.03 | precursor -98 - 409.7108++ | 11.35 | 7.56 | 8.29 | 13.99 | 10.42 | 14.18 | ||
| R.SGSVGNYR.T [488, 495] | non-bound | 2.78E+03 | -7.65E+02 | 0.9745 | 0.2835 | 0.9451 | 2.58 | G [y4] - 509.2467+ | 4.95 | 3.90 | 3.87 | 5.34 | 4.57 | 4.80 | ||
| R.SGS[+80]VGNYR.T [488, 495] | bound | 1.07E+03 | -1.93E+02 | 0.9945 | 0.2221 | 0.7403 | 2.18 | G [y4] - 519.2549+ | 5.79 | 2.43 | 2.24 | 6.54 | 5.06 | 4.48 | ||
| prkaa2/AMPK2 | R.SGS[+80]TAGLLRPR.L [488, 498] | bound | 3.98E+03 | -1.91E+03 | 0.9860 | 0.5522 | 1.8407 | 1.78 | precursor -98 - 553.8189++ | 10.22 | 3.12 | 3.29 | 13.57 | 5.96 | 6.02 | |
| prkaa1/2 | K.IADFGLSNMMSDGEFLR.T [156, 172] | non-bound | 4.28E+00 | -2.35E+01 | 0.9406 | 8.3225 | 27.7417 | 1.11 | S [y11] - 1286.5504+ | 6.05 | 9.85 | |||||
| K.IADFGLS[+80]NMMSDGEFLR.T [156, 172] | bound | 5.55E+01 | -6.25E+01 | 0.8912 | 1.8375 | 6.1250 | 1.26 | precursor -98 - 947.9387++ | 22.45 | 7.74 | 58.43 | 9.13 | ||||
| R.TSC[+57]GSPNYAAPEVISGR.L [173, 189] | non-bound | 2.80E+02 | 4.95E+01 | 0.9870 | 0.0212 | 0.0707 | 3.70 | P [y7] - 757.4203+ | 8.06 | 3.53 | 2.40 | 9.57 | 4.17 | 2.88 | ||
| R.T[+80]SC[+57]GSPNYAAPEVISGR.L [173, 189] | bound | 3.28E+02 | -1.13E+02 | 0.9710 | 0.5664 | 1.8881 | 1.77 | precursor -98 - 879.4112++ | 15.99 | 6.78 | 6.23 | 16.11 | 7.76 | 9.32 | ||
| prkag1/AMPKg | K.LVVFDTSLQVK.K [46, 56] | non-bound | 3.01E+03 | -1.70E+03 | 0.9446 | 5.8910 | 19.6367 | 1.26 | V [y9] - 1036.5673+ | 15.21 | 13.44 | 5.38 | 29.34 | 54.32 | 9.75 | |
| R.SQYFEGVLTC[+57]R.A [268, 278] | non-bound | 1.09E+03 | -1.49E+03 | 0.9941 | 1.4642 | 4.8807 | 1.86 | G [y6] - 705.3712+ | 5.31 | 2.61 | 1.97 | 5.70 | 2.95 | 2.34 | ||
| R.SQY[+80]FEGVLTC[+57]R.A [268, 278] | bound | 2.54E+02 | -2.89E+01 | 0.9915 | 0.5896 | 1.9653 | 1.75 | L [y4] - 559.2896+ | 3.90 | 2.77 | 7.45 | 4.56 | ||||
| R.ASETLQAIINR.L [279, 289] | non-bound | 2.15E+03 | -1.61E+03 | 0.9959 | 0.9507 | 3.1691 | 2.05 | Q [y6] - 714.4257+ | 4.37 | 2.18 | 1.47 | 5.88 | 3.93 | 2.85 | ||
| R.ASET[+80]LQAIINR.L [279, 289] | bound | 1.41E+03 | -2.37E+02 | 0.9901 | 0.2776 | 0.9254 | 2.08 | precursor -98 - 604.3371++ | 8.42 | 4.91 | 5.79 | 10.35 | 5.87 | 8.36 | ||
| rheb | K.LLDMVGK.V [103, 109] | non-bound | 4.61E+01 | 2.97E+01 | 0.9522 | 0.6492 | 2.1640 | 2.22 | D [y5] - 549.2701+ | 8.43 | 8.04 | 11.96 | 12.55 | |||
| R.VISC[+57]EEGK.A [128, 135] | non-bound | 2.15E+03 | -5.41E+02 | 0.9725 | 0.2606 | 0.8688 | 2.61 | S [y6] - 709.2821+ | 4.78 | 4.77 | 5.88 | 5.41 | 5.96 | 7.46 | ||
| R.VIS[+80]C[+57]EEGK.A [128, 135] | bound | 1.11E+03 | -8.82E+01 | 0.9808 | 0.1161 | 0.3869 | 2.46 | precursor -98 - 456.2228++ | 8.37 | 6.03 | 6.50 | 9.84 | 6.23 | 9.85 | ||
| rictorb | K.TLDTQDSTSS[+80]IGEQEVR.L [1186, 1202] | bound | 1.39E+02 | 1.15E+00 | 0.9944 | 0.2739 | 0.9129 | 2.09 | G [y6] - 727.3609+ | 23.25 | 6.50 | 1.96 | 28.26 | 11.71 | 4.57 | |
| K.ALS[+80]FASLDK.E [1434, 1442] | bound | 3.05E+03 | 5.33E+02 | 0.9692 | 0.0267 | 0.0890 | 3.10 | precursor -98 - 471.2627++ | 9.67 | 5.74 | 6.24 | 11.04 | 6.93 | 12.77 | ||
| R.S[+80]TELVLGVK.N [1631, 1639] | bound | 3.07E+03 | 2.01E+03 | 0.9770 | 0.2243 | 0.7476 | 2.17 | precursor -98 - 468.2862++ | 12.35 | 5.84 | 8.24 | 15.087489 | 8.107909703 | 14.48 | ||
| rps6 | K.LIEVDDER.K [15, 22] | non-bound | 4.63E+03 | -6.19E+00 | 0.9891 | 0.0059 | 0.0198 | 3.75 | E [y6] - 772.3347+ | 5.37 | 2.87 | 3.30 | 6.50 | 3.37 | 3.98 | |
| R.RLSSLRAS[+80]TSK.S [233, 243] (missed 2) | bound | 3.68E+02 | -9.48E+02 | 0.9655 | 2.6973 | 8.9910 | 1.09 | precursor -98 - 598.3535++ | 5.92 | 5.90 | 6.76 | 7.96 | ||||
| R.LSS[+80]LRASTSK.S [234, 243] (missed 1) | bound | 2.17E+03 | -7.34E+02 | 0.9901 | 0.3483 | 1.1611 | 1.98 | precursor -98 - 520.3029++ | 6.12 | 2.95 | 2.40 | 8.62 | 4.58 | 2.93 | ||
| R.LS[+80]SLRAS[+80]TSK.S [234, 243] (missed 1) | bound | 6.20E+03 | -2.36E+03 | 0.9756 | 0.4264 | 1.4215 | 1.89 | precursor -98 - 556.2790++ | 24.92 | 18.14 | 5.05 | 28.86 | 22.49 | 7.36 | ||
| rps6kb1b | K.FTSQTPVDSPDDTK.L [360, 373] | non-bound | 5.01E+02 | -7.87E+02 | 0.9938 | 1.6176 | 5.3920 | 1.82 | P [y9] - 973.4473+ | 7.89 | 2.49 | 1.80 | 9.31 | 4.05 | 2.30 | |
| K.FTSQT[+80]PVDSPDDTK.L [360, 373] | bound | 8.20E+01 | -1.03E+01 | 0.9940 | 0.4106 | 1.3687 | 1.91 | precursor -98 - 764.3563++ | 12.92 | 2.59 | 17.81 | 3.22 | ||||
| R.KVLGS[+80]PR.T [411, 417] (missed 1) | bound | 1.55E+03 | -3.79E+02 | 0.9918 | 0.2625 | 0.8749 | 2.10 | precursor -98 - 374.7388++ | 10.15 | 7.37 | 8.12 | 12.99 | 12.77 | 15.61 | ||
| K.VLGS[+80]PR.T [412, 417] | bound | 2.75E+03 | 5.26E+01 | 0.9512 | 0.0035 | 0.0117 | 3.98 | G [y4 -98] - 408.2229+ | 3.02 | 2.64 | 2.86 | 4.46 | 3.61 | 3.27 | ||
| R.TPVSPLK.A [418, 424] | non-bound | 8.33E+03 | -1.16E+03 | 0.9723 | 0.1672 | 0.5574 | 2.81 | P [y6] - 320.7051++ | 4.70 | 4.09 | 5.87 | 5.94 | 5.13 | 8.90 | ||
| R.TPVS[+80]PLK.A [418, 424] | bound | 1.84E+03 | -4.29E+01 | 0.9718 | 0.0857 | 0.2857 | 2.59 | precursor -98 - 366.2307++ | 8.99 | 7.23 | 9.11 | 12.38 | 10.68 | 17.49 | ||
| K.AEVEDGWSR.G [425, 433] | non-bound | 1.35E+03 | -7.16E+02 | 0.9900 | 0.6187 | 2.0622 | 2.24 | E [y6] - 749.3213+ | 7.23 | 3.14 | 2.26 | 8.12 | 4.95 | 2.83 | ||
| rptor | K.ALETIGANLQK.Q [100, 110] | non-bound | 5.56E+03 | -9.93E+02 | 0.9921 | 0.1902 | 0.6339 | 2.24 | G [y6] - 638.3711+ | 6.32 | 3.07 | 3.49 | 7.04 | 3.34 | 5.06 | |
| R.SVNSYTNLR.A [722, 730] | non-bound | 1.78E+03 | -1.21E+03 | 0.9922 | 0.6997 | 2.3324 | 2.19 | N [y7] - 867.4319+ | 4.57 | 2.25 | 2.37 | 5.26 | 2.71 | 2.77 | ||
| R.SVNS[+80]YTNLR.A [722, 730] | bound | 5.02E+02 | -6.39E+01 | 0.9776 | 0.1840 | 0.6135 | 2.26 | N [y7 -98] - 859.4296+ | 9.15 | 7.00 | 5.37 | 14.18 | 8.18 | 8.66 | ||
| R.ILDSGSLTQSAPASPTSK.G [852, 869] | non-bound | 2.12E+03 | -1.10E+02 | 0.9878 | 0.0729 | 0.2432 | 3.17 | P [y7] - 687.3672+ | 5.26 | 2.22 | 2.54 | 7.56 | 3.88 | 3.11 | ||
| R.ILDSGSLTQSAPAS[+80]PTSK.G [852, 869] | bound | 1.91E+02 | -6.65E+00 | 0.9889 | 0.1326 | 0.4421 | 2.40 | P [y7] - 775.3477+ | 24.82 | 4.02 | 4.76 | 27.28 | 5.17 | 6.64 | ||
| R.SLVAAGLGDGSVR.V [1207, 1219] | non-bound | 2.21E+03 | -1.28E+03 | 0.9939 | 0.7002 | 2.3341 | 2.18 | G [y6] - 590.2893+ | 4.91 | 2.91 | 3.23 | 6.42 | 3.46 | 4.68 | ||
| stk11 | R.IDSTEVIYQPR.R [28, 38] | non-bound | 1.83E+03 | -2.09E+03 | 0.9913 | 1.1786 | 3.9287 | 1.96 | I [y5] - 676.3777+ | 6.11 | 2.52 | 2.06 | 6.84 | 3.45 | 2.51 | |
| R.IDS[+80]TEVIYQPR.R [28, 38] | bound | 7.37E+02 | -1.54E+02 | 0.9894 | 0.3052 | 1.0173 | 2.04 | precursor -98 - 656.8422++ | 10.83 | 6.44 | 5.61 | 12.22 | 9.02 | 10.89 | ||
| R.GMLEYDPVK.R [287, 295] | non-bound | 3.32E+02 | -1.19E+02 | 0.9744 | 0.4233 | 1.4110 | 2.40 | E [y6] - 750.3668+ | 10.78 | 2.46 | 3.73 | 12.67 | 3.05 | 4.44 | ||
| tsc1b | R.EQLNVGDLLPLLETSDLR.Q [4, 21] | non-bound | 1.22E+03 | -3.30E+03 | 0.9289 | 2.7981 | 9.3270 | 1.58 | P [y9] - 522.2902++ | 15.72 | 30.44 | 5.31 | 54.07 | 74.30 | 7.47 | |
| R.STAS[+80]PPC[+57]K.D [434, 441] | bound | 3.70E+02 | -1.20E+02 | 0.9899 | 0.3614 | 1.2048 | 1.96 | A [y6 -98] - 649.3218+ | 15.89 | 4.05 | 4.02 | 18.84 | 8.38 | 8.29 | ||
| K.SES[+80]QC[+57]DEEETPPPR.L [1137, 1150] | bound | 1.50E+03 | -3.69E+02 | 0.9960 | 0.2807 | 0.9358 | 2.07 | T [b10] - 638.1896++ | 5.90 | 3.46 | 2.71 | 6.28 | 5.08 | 3.58 | ||
| tsc2 | K.NTPTLVLPSFYK.A [147, 158] | non-bound | 6.23E+03 | -7.45E+03 | 0.9702 | 1.2196 | 4.0653 | 1.94 | P [y10] - 582.8368++ | 6.42 | 4.31 | 6.41 | 13.89 | 5.65 | 11.14 | |
| R.STSLNERPK.S [769, 777] | non-bound | 4.26E+02 | -8.93E+02 | 0.9619 | 2.1827 | 7.2757 | 1.69 | N [y5] - 643.3522+ | 12.41 | 5.80 | 6.61 | 18.24 | 12.61 | 13.80 | ||
| R.STS[+80]LNERPK.S [769, 777] | bound | 5.53E+02 | -2.36E+02 | 0.9865 | 0.5022 | 1.6740 | 1.82 | precursor -98 - 511.2795++ | 12.47 | 5.11 | 5.68 | 15.35 | 5.80 | 7.34 | ||
| R.SISVSEHAVR.R [811, 820] | non-bound | 7.12E+02 | -1.40E+03 | 0.9904 | 2.0428 | 6.8093 | 1.72 | S [y8] - 884.4585+ | 8.62 | 4.29 | 4.51 | 9.98 | 8.43 | 9.37 | ||
| R.SIS[+80]VSEHAVR.R [811, 820] | bound | 2.32E+03 | -9.49E+02 | 0.9904 | 0.4688 | 1.5627 | 1.85 | precursor -98 - 538.7898++ | 5.91 | 3.01 | 2.60 | 7.52 | 4.04 | 3.06 | ||
| R.SISGGHALR.A [962, 970] | non-bound | 2.07E+03 | -3.52E+03 | 0.9915 | 1.7168 | 5.7227 | 1.80 | S [y7] - 697.3740+ | 7.03 | 2.74 | 2.20 | 9.81 | 5.74 | 4.90 | ||
| R.SIS[+80]GGHALR.A [962, 970] | bound | 3.39E+03 | -1.33E+03 | 0.9839 | 0.4387 | 1.4624 | 1.88 | precursor -98 - 445.2475++ | 4.77 | 2.88 | 3.57 | 7.75 | 3.26 | 4.04 | ||
| K.SSSSPELQTLPEAFSK.A [1240, 1255] | non-bound | 8.03E+02 | 1.55E+02 | 0.9856 | 0.0025 | 0.0083 | 4.63 | P [y6] - 678.3457+ | 4.70 | 3.29 | 2.99 | 8.78 | 4.49 | 3.58 | ||
| K.S[+80]SSSPELQTLPEAFSK.A [1240, 1255] | bound | 2.75E+02 | 3.99E+01 | 0.9745 | 0.3514 | 1.1713 | 1.98 | P [y12] - 684.3685++ | 9.64 | 3.20 | 12.14 | 4.02 | ||||
| R.GHTISVSAPSSR.R [1327, 1338] | non-bound | 2.15E+02 | -6.33E+02 | 0.9867 | 2.9862 | 9.9540 | 1.55 | T [y10] - 1004.5371+ | 15.13 | 5.35 | 4.27 | 18.43 | 7.50 | 6.63 | ||
| R.GHT[+80]ISVSAPSSR.R [1327, 1338] | bound | 1.04E+03 | 5.13E+01 | 0.9852 | 0.0070 | 0.0233 | 3.68 | P [y4] - 456.2440+ | 17.26 | 7.36 | 8.65 | 17.96 | 8.41 | 9.13 | ||
| R.YAQFLTGLGK.L [1436, 1445] | non-bound | 4.83E+03 | -2.24E+03 | 0.9786 | 0.4804 | 1.6013 | 2.35 | F [y7] - 735.4400+ | 3.62 | 2.47 | 2.77 | 5.51 | 3.74 | 3.35 | ||
| ulk1b | R.LNS[+80]APC[+57]LLEAAGGC[+57]R.Q [549, 563] | bound | 3.78E+02 | -1.82E+02 | 0.9846 | 0.5796 | 1.9321 | 1.76 | precursor -98 - 790.8810++ | 16.91 | 5.88 | 3.47 | 24.45 | 6.38 | 4.39 | |
| R.NPLPTILGSPSR.A [600, 611] | non-bound | 4.72E+03 | -5.48E+03 | 0.9985 | 1.1721 | 3.9070 | 1.96 | P [y9] - 927.5258+ | 3.90 | 1.76 | 1.57 | 6.51 | 3.37 | 2.93 | ||
| R.VVFTIGS[+80]PPSGATPPQNSR.S [715, 733] | bound | 6.02E+02 | -9.31E+01 | 0.9935 | 0.3490 | 1.1632 | 1.98 | P [y6] - 354.6868++ | 8.06 | 3.57 | 9.42 | 4.94 | ||||
| mean | median | median | median | median | median | median | median | median | ||||||||
| non-bound | 0.9790 | 1.0838 | 3.6127 | 6.55 | 3.53 | 3.30 | 9.31 | 5.13 | 4.87 | |||||||
| bound | 0.9718 | 0.3559 | 1.1863 | 11.69 | 5.97 | 4.64 | 14.86 | 8.28 | 7.35 | |||||||
| deviation | deviation | deviation | deviation | deviation | deviation | |||||||||||
| 5.02 | 4.77 | 1.88 | 14.41 | 12.28 | 3.59 | |||||||||||
| 5.61 | 4.08 | 3.28 | 8.79 | 10.20 | 5.01 | |||||||||||